MetaCyc Enzyme: glutamate dehydrogenase (NADP+)

Gene: gdh Accession Number: G-10249 (MetaCyc)

Species: Neurospora crassa

Subunit composition of glutamate dehydrogenase (NADP+) = [Gdh]6
         glutamate dehydrogenase subunit = Gdh

Different species of yeasts and filamentous fungi, including Neurospora crassa, possess two glutamate dehydrogenases. One depends on NADP and has a biosynthetic role, whereas the other depends on NAD and catabolizes glutamate. Like the vertebrate enzymes, glutamate dehydrogenase (NADP+) of Neurospora crassa is a homohexamer, composed of 48.8 kDa subunits [Blumenthal73].

Unlike the vertebrate enzyme, the enzyme from Neurospora crassa exhibits very weak, if any, allosteric effects.

Gene Citations: [Kinnaird83]

Molecular Weight of Polypeptide: 48.853 kD (from nucleotide sequence), 48.8 kD (experimental) [Blumenthal73 ]

Molecular Weight of Multimer: 288.4 kD (experimental) [Blumenthal73]

Unification Links: Pride:P00369 , Protein Model Portal:P00369 , SMR:P00369 , String:5141.NCU01195.1 , UniProt:P00369

Relationship Links: Entrez-Nucleotide:PART-OF:K01409 , InterPro:IN-FAMILY:IPR006095 , InterPro:IN-FAMILY:IPR006096 , InterPro:IN-FAMILY:IPR006097 , InterPro:IN-FAMILY:IPR014362 , InterPro:IN-FAMILY:IPR016040 , Pfam:IN-FAMILY:PF00208 , Pfam:IN-FAMILY:PF02812 , Prints:IN-FAMILY:PR00082 , Prosite:IN-FAMILY:PS00074 , Smart:IN-FAMILY:SM00839

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 17-Oct-2007 by Caspi R , SRI International

Enzymatic reaction of: glutamate dehydrogenase

Synonyms: NADP-dependent glutamate dehydrogenase

EC Number:

L-glutamate + NADP+ + H2O <=> ammonium + 2-oxoglutarate + NADPH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

This reaction is reversible.

In Pathways: nitrate reduction V (assimilatory)

Inhibitors (Other): pyridoxal 5'-phosphate [Blumenthal73] , N-ethylmaleimide [Blumenthal73]


Blumenthal73: Blumenthal KM, Smith EL (1973). "Nicotinamide adenine dinucleotide phosphate-specific glutamate dehydrogenase of Neurospora. I. Isolation, subunits, amino acid composition, sulfhydryl groups, and identification of a lysine residue reactive with pyridoxal phosphate and N-ethylmaleimide." J Biol Chem 248(17);6002-8. PMID: 4146914

Kinnaird83: Kinnaird JH, Fincham JR (1983). "The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene." Gene 26(2-3);253-60. PMID: 6231215

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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