MetaCyc Enzyme: dimethylmaleate hydratase

Species: Eubacterium barkeri

Subunit composition of dimethylmaleate hydratase = [DmdA][DmdB]
         dimethylmaleate hydratase subunit A = DmdA
         dimethylmaleate hydratase subunit B = DmdB

The native molecular weight of the partially purified enzyme was estimated by gel filtration chromatography. However, the subunit composition was not determined [KollmannKoch84].

Gene-Reaction Schematic

Gene-Reaction Schematic

Enzymatic reaction of: dimethylmaleate hydratase

Inferred from experiment

Synonyms: (2R,3S)-dimethylmalate dehydratase, (2R,3S)-2,3-dimethylmalate hydro-lyase

EC Number:

(2R,3S)-2,3-dimethylmalate ⇄ dimethylmaleate + H2O

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for dimethylmaleate: citraconate [KollmannKoch84]

In Pathways: superpathway of nicotinate degradation, nicotinate degradation III

The enzyme is induced by growth of the organism on nicotinate. It is oxygen-sensitive and is dependent upon ferrous ion. The reaction was reversible, but the equilibrium was 70% in favor of dimethylmalate. This product was determined to have a (2R,3S) configuration [KollmannKoch84].

Cofactors or Prosthetic Groups: Fe2+ [KollmannKoch84]

Inhibitors (Unknown Mechanism): 5,5'-dithio-bis-2-nitrobenzoate [KollmannKoch84], N-ethylmaleimide [KollmannKoch84]Kinetic Parameters:
Substrate Km (μM) Citations
dimethylmaleate 3100.0 [KollmannKoch84]

Subunit of dimethylmaleate hydratase: dimethylmaleate hydratase subunit A

Synonyms: DmdA

Gene: dmdA Accession Number: G-10436 (MetaCyc)

Molecular Weight: 44.821 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q0QLE2, UniProt:Q0QLE2

Relationship Links: InterPro:IN-FAMILY:IPR001030, InterPro:IN-FAMILY:IPR006251, InterPro:IN-FAMILY:IPR011823, InterPro:IN-FAMILY:IPR011826, InterPro:IN-FAMILY:IPR015931, InterPro:IN-FAMILY:IPR015932, InterPro:IN-FAMILY:IPR015937, InterPro:IN-FAMILY:IPR018136, Panther:IN-FAMILY:PTHR11670, Pfam:IN-FAMILY:PF00330, Prints:IN-FAMILY:PR00415, Prosite:IN-FAMILY:PS00450, Prosite:IN-FAMILY:PS01244

Subunit of dimethylmaleate hydratase: dimethylmaleate hydratase subunit B

Synonyms: DmdB

Gene: dmdB Accession Number: G-10437 (MetaCyc)

Molecular Weight: 17.369 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q0QLE1, UniProt:Q0QLE1

Relationship Links: InterPro:IN-FAMILY:IPR000573, InterPro:IN-FAMILY:IPR011824, InterPro:IN-FAMILY:IPR011827, InterPro:IN-FAMILY:IPR015928, InterPro:IN-FAMILY:IPR015937, Panther:IN-FAMILY:PTHR11670, Pfam:IN-FAMILY:PF00694


KollmannKoch84: Kollmann-Koch A, Eggerer H (1984). "Nicotinic acid metabolism. Dimethylmaleate hydratase." Hoppe Seylers Z Physiol Chem 365(8);847-57. PMID: 6489933

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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