MetaCyc Enzyme: chlorocatechol 1,2-dioxygenase

Gene: clcA Accession Number: G-11002 (MetaCyc)

Species: Pseudomonas putida AC866

Subunit composition of chlorocatechol 1,2-dioxygenase = [ClcA]2
         chlorocatechol 1,2-dioxygenase subunit = ClcA

Plasmid pAC25 is a catabolic plsmid isolated from Pseudomonas putida AC866, a strain capable of degrading several chlorinated aromatic compounds [Chatterjee81]. The three genes on the plasmid encode chlorocatechol dioxygenase, muconate cycloisomerase II, and dienelactone hydrolase, allowing for the degradation chlorocatechols.

The clcA gene, which encodes chlorocatechol 1,2-dioxygenase, has been cloned and the product was purified to homogeneity [Broderick91]. The active protein is a homodimer, with the two dimers linked around one atom of Fe3+. The highest specific activities of the purified enzyme were observed with 3-methylcatechol and 4-methylcatechol (29.8 and 22.9 units/mg, respectively), while the specific activities with the physiological substrates 3-chlorocatechol and 4-chlorocatechol were significantly lower (10.2 and 6.25 units/mg, respectively) [Broderick91].

Gene Citations: [Muller96]

Molecular Weight of Polypeptide: 28.988 kD (from nucleotide sequence), 33.0 kD (experimental) [Frantz87 ]

Molecular Weight of Multimer: 57.5 kD (experimental) [Broderick91]

pI: 5.8 [Broderick91]

Unification Links: Protein Model Portal:P11451 , UniProt:P11451

Relationship Links: Entrez-Nucleotide:PART-OF:M16964 , InterPro:IN-FAMILY:IPR000627 , InterPro:IN-FAMILY:IPR007535 , InterPro:IN-FAMILY:IPR012817 , InterPro:IN-FAMILY:IPR015889 , PDB:Structure:3TH1 , Pfam:IN-FAMILY:PF00775 , Pfam:IN-FAMILY:PF04444 , Prosite:IN-FAMILY:PS00083

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 14-Nov-2008 by Caspi R , SRI International

Enzymatic reaction of: 4-chlorocatechol 1,2-dioxygenase

4-chlorocatechol + oxygen <=> 3-chloro-cis,cis-muconate + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

In Pathways: 4-chlorocatechol degradation

Enzymatic reaction of: 3-chlorocatechol 1,2-dioxygenase

3-chlorocatechol + oxygen <=> 2-chloro-cis,cis-muconate + 2 H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for 3-chlorocatechol: 3-methylcatechol [Broderick91 ] , 4-methylcatechol [Broderick91 ]

In Pathways: 3-chlorocatechol degradation I (ortho)

Cofactors or Prosthetic Groups: Fe3+ [Araujo00]

pH(opt): 7.5-7.9 [Broderick91]


Araujo00: Araujo AP, Oliva G, Henrique-Silva F, Garratt RC, Caceres O, Beltramini LM (2000). "Influence of the histidine tail on the structure and activity of recombinant chlorocatechol 1,2-dioxygenase." Biochem Biophys Res Commun 272(2);480-4. PMID: 10833439

Broderick91: Broderick JB, O'Halloran TV (1991). "Overproduction, purification, and characterization of chlorocatechol dioxygenase, a non-heme iron dioxygenase with broad substrate tolerance." Biochemistry 30(29);7349-58. PMID: 1649626

Chatterjee81: Chatterjee DK, Kellogg ST, Hamada S, Chakrabarty AM (1981). "Plasmid specifying total degradation of 3-chlorobenzoate by a modified ortho pathway." J Bacteriol 146(2);639-46. PMID: 7217013

Frantz87: Frantz B, Chakrabarty AM (1987). "Organization and nucleotide sequence determination of a gene cluster involved in 3-chlorocatechol degradation." Proc Natl Acad Sci U S A 84(13);4460-4. PMID: 3299368

Muller96: Muller D, Schlomann M, Reineke W (1996). "Maleylacetate reductases in chloroaromatic-degrading bacteria using the modified ortho pathway: comparison of catalytic properties." J Bacteriol 178(1);298-300. PMID: 8550433

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc13.