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Metabolic Modeling Tutorial
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MetaCyc Enzyme: 2-aminophenol 1,6-dioxygenase

Species: Pseudomonas sp. AP3

Subunit composition of 2-aminophenol 1,6-dioxygenase = [AmnA]2[AmnB]2
         2-aminophenol 1,6-dioxygenase α subunit = AmnA
         2-aminophenol 1,6-dioxygenase β subunit = AmnB

Summary:
2-aminophenol 1,6-dioxygenase has been purified to homogeneity from Pseudomonas sp. AP3 [Takenaka97]. The enzyme is a homotetramer, composed of two types of subunits, and contains 1 mole of Fe2+ per mole tetramer. Substrate range was rather narrow, although the enzyme did accept several alternative substrates, such as 2-amino-5-methylphenol, with much lower activity.

The amnA and amnB genes encoding the enzyme were cloned and sequenced [Takenaka97].

Molecular Weight: 140.0 kD (experimental) [Takenaka97 ]

Gene-Reaction Schematic: ?

Credits:
Created 07-Apr-2009 by Caspi R , SRI International


Enzymatic reaction of: 2-aminophenol 1,6-dioxygenase

EC Number: 1.13.11.74

2-aminophenol + oxygen <=> 2-aminomuconate 6-semialdehyde + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for 2-aminophenol: 2-amino-5-methylphenol [Takenaka97 ]

In Pathways: 2-aminophenol degradation

Cofactors or Prosthetic Groups: Fe2+ [Takenaka97]

Inhibitors (Unknown Mechanism): Mn2+ [Takenaka97] , Cu2+ [Takenaka97] , Fe3+ [Takenaka97] , Ag+ [Takenaka97] , Hg2+ [Takenaka97]

Kinetic Parameters:

Substrate
Km (μM)
Citations
2-aminophenol
46.7
[Takenaka97]


Subunit of 2-aminophenol 1,6-dioxygenase: 2-aminophenol 1,6-dioxygenase α subunit

Synonyms: AmnA

Gene: amnA Accession Number: G-11248 (MetaCyc)

Molecular Weight: 29.416 kD (from nucleotide sequence)

Molecular Weight: 32.0 kD (experimental) [Takenaka97]

Unification Links: Protein Model Portal:O33478 , UniProt:O33478

Relationship Links: InterPro:IN-FAMILY:IPR004183 , Pfam:IN-FAMILY:PF02900


Subunit of 2-aminophenol 1,6-dioxygenase: 2-aminophenol 1,6-dioxygenase β subunit

Synonyms: AmnB

Gene: amnB Accession Number: G-11249 (MetaCyc)

Molecular Weight: 34.441 kD (from nucleotide sequence)

Molecular Weight: 40.0 kD (experimental) [Takenaka97]

Unification Links: Protein Model Portal:O33477 , UniProt:O33477

Relationship Links: InterPro:IN-FAMILY:IPR004183 , Pfam:IN-FAMILY:PF02900


References

Takenaka97: Takenaka S, Murakami S, Shinke R, Hatakeyama K, Yukawa H, Aoki K (1997). "Novel genes encoding 2-aminophenol 1,6-dioxygenase from Pseudomonas species AP-3 growing on 2-aminophenol and catalytic properties of the purified enzyme." J Biol Chem 272(23);14727-32. PMID: 9169437


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, biocyc13.