MetaCyc Enzyme: 2-aminophenol 1,6-dioxygenase

Species: Pseudomonas sp. AP3

Subunit composition of 2-aminophenol 1,6-dioxygenase = [AmnA]2[AmnB]2
         2-aminophenol 1,6-dioxygenase α subunit = AmnA
         2-aminophenol 1,6-dioxygenase β subunit = AmnB

2-aminophenol 1,6-dioxygenase has been purified to homogeneity from Pseudomonas sp. AP3 [Takenaka97]. The enzyme is a homotetramer, composed of two types of subunits, and contains 1 mole of Fe2+ per mole tetramer. Substrate range was rather narrow, although the enzyme did accept several alternative substrates, such as 2-amino-5-methylphenol, with much lower activity.

The amnA and amnB genes encoding the enzyme were cloned and sequenced [Takenaka97].

Molecular Weight: 140.0 kD (experimental) [Takenaka97]

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 07-Apr-2009 by Caspi R, SRI International

Enzymatic reaction of: 2-aminophenol 1,6-dioxygenase

Inferred from experiment

EC Number:

2-aminophenol + oxygen → 2-aminomuconate 6-semialdehyde + H+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for 2-aminophenol: 2-amino-5-methylphenol [Takenaka97]

In Pathways: 2-aminophenol degradation

Cofactors or Prosthetic Groups: Fe2+ [Takenaka97]

Inhibitors (Unknown Mechanism): Mn2+ [Takenaka97], Cu2+ [Takenaka97], Fe3+ [Takenaka97], Ag+ [Takenaka97], Hg2+ [Takenaka97]Kinetic Parameters:
Substrate Km (μM) Citations
2-aminophenol 46.7 [Takenaka97]

Subunit of 2-aminophenol 1,6-dioxygenase: 2-aminophenol 1,6-dioxygenase α subunit

Synonyms: AmnA

Gene: amnA Accession Number: G-11248 (MetaCyc)

Molecular Weight: 29.416 kD (from nucleotide sequence)

Molecular Weight: 32.0 kD (experimental) [Takenaka97]

Unification Links: Protein Model Portal:O33478, UniProt:O33478

Relationship Links: InterPro:IN-FAMILY:IPR004183, Pfam:IN-FAMILY:PF02900

Subunit of 2-aminophenol 1,6-dioxygenase: 2-aminophenol 1,6-dioxygenase β subunit

Synonyms: AmnB

Gene: amnB Accession Number: G-11249 (MetaCyc)

Molecular Weight: 34.441 kD (from nucleotide sequence)

Molecular Weight: 40.0 kD (experimental) [Takenaka97]

Unification Links: Protein Model Portal:O33477, UniProt:O33477

Relationship Links: InterPro:IN-FAMILY:IPR004183, Pfam:IN-FAMILY:PF02900


Takenaka97: Takenaka S, Murakami S, Shinke R, Hatakeyama K, Yukawa H, Aoki K (1997). "Novel genes encoding 2-aminophenol 1,6-dioxygenase from Pseudomonas species AP-3 growing on 2-aminophenol and catalytic properties of the purified enzyme." J Biol Chem 272(23);14727-32. PMID: 9169437

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Wed Nov 25, 2015, BIOCYC11A.