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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: 4-oxalocrotonate decarboxylase

Gene: amnE Accession Number: G-11252 (MetaCyc)

Species: Pseudomonas sp. AP3

Subunit composition of 4-oxalocrotonate decarboxylase = [AmnE]
         4-oxalocrotonate decarboxylase monomer = AmnE

Summary:
4-oxalocrotonate decarboxylase has been purified from Pseudomonas sp. AP3 to homogeneity [Takenaka00]. The protein was found to form a large complex (300 kDa) with the hydratase encoded by amnF, which catalyzes the next step of the pathway. The ratio of decarboxylase to hydratase subunits within the complex was 1:1.

The enzyme required either Mg2+ or Mn2+ for activity, and was sensitive to several metal ions, including Fe2+, Fe3+, Ag+ and Hg2+ [Takenaka00].

Molecular Weight of Polypeptide: 27.456 kD (from nucleotide sequence), 29.0 kD (experimental) [Takenaka00 ]

Unification Links: Protein Model Portal:Q9KWS3 , UniProt:Q9KWS3

Relationship Links: Entrez-Nucleotide:PART-OF:AB020521 , InterPro:IN-FAMILY:IPR002529 , InterPro:IN-FAMILY:IPR011234 , Pfam:IN-FAMILY:PF01557

Gene-Reaction Schematic: ?

Credits:
Created 07-Apr-2009 by Caspi R , SRI International
Revised 08-Apr-2009 by Caspi R , SRI International


Enzymatic reaction of: 4-oxalocrotonate decarboxylase

EC Number: 4.1.1.77

(3E)-2-oxohex-3-enedioate + H+ <=> 2-oxopent-4-enoate + CO2

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: 2-aminophenol degradation

Cofactors or Prosthetic Groups: Mg2+ [Takenaka00], Mn2+ [Takenaka00]

Inhibitors (Unknown Mechanism): Fe2+ [Takenaka00] , Fe3+ [Takenaka00] , Ag+ [Takenaka00] , Cu2+ [Takenaka00]

pH(opt): 7.5 [Takenaka00]


References

Takenaka00: Takenaka S, Murakami S, Kim YJ, Aoki K (2000). "Complete nucleotide sequence and functional analysis of the genes for 2-aminophenol metabolism from Pseudomonas sp. AP-3." Arch Microbiol 174(4);265-72. PMID: 11081795


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC13B.