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MetaCyc Enzyme: carboxyphosphonoenolpyruvate phosphonomutase

Gene: bcpA Accession Number: G-11484 (MetaCyc)

Species: Streptomyces hygroscopicus

Subunit composition of carboxyphosphonoenolpyruvate phosphonomutase = [BcpA]2
         carboxyphosphonoenolpyruvate phosphonomutase monomer = BcpA

Summary:
carboxyphosphonoenolpyruvate phosphonomutase catalyzes a key step in the biosynthesis of the antibiotic phophinothricin tripeptide - the rearrangement of the carboxyphosphono group of carboxyphosphonoenolpyruvate to form the unusual C-P bond of phosphinopyruvate, followed by decarboxylation.

The enzyme has been isolated from the cell extract of a mutant (NP71) of Streptomyces hygroscopicus and purified purified about 200-fold [Hidaka90].

The bcpA gene that encodes the enzyme was identified by complementation of a second mutant and sequenced [Hidaka92, Pollack92]. The gene was also expressed in Streptomyces lividans [Hidaka90] and in Escherichia coli [Pollack92].

Gene Citations: [Anzai87]

Molecular Weight of Polypeptide: 32.781 kD (from nucleotide sequence), 32.0 kD (experimental) [Hidaka90 ]

Unification Links: Entrez-Nucleotide:D00609 , Protein Model Portal:P11435 , UniProt:P11435

Relationship Links: InterPro:IN-FAMILY:IPR012697 , InterPro:IN-FAMILY:IPR015813 , InterPro:IN-FAMILY:IPR018523 , Pfam:IN-FAMILY:PF00463 , Prosite:IN-FAMILY:PS00161

Gene-Reaction Schematic: ?

Credits:
Created 26-Aug-2009 by Caspi R , SRI International


Enzymatic reaction of: carboxyphosphinopyruvate decarboxylase (carboxyphosphonoenolpyruvate phosphonomutase)

carboxyphosphinopyruvate <=> phosphinopyruvate + CO2

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: phosphinothricin tripeptide biosynthesis


Enzymatic reaction of: carboxyphosphonoenolpyruvate phosphonomutase

carboxyphosphonoenolpyruvate <=> carboxyphosphinopyruvate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: phosphinothricin tripeptide biosynthesis

Activators (Unknown Mechanism): Mn2+ [Hidaka90] , Mg2+ [Hidaka90]

Kinetic Parameters:

Substrate
Km (μM)
Citations
carboxyphosphonoenolpyruvate
250.0
[Hidaka90]

pH(opt): 6.5 [Hidaka90]


References

Anzai87: Anzai H, Murakami T, Imai S, Satoh A, Nagaoka K, Thompson CJ (1987). "Transcriptional regulation of bialaphos biosynthesis in Streptomyces hygroscopicus." J Bacteriol 169(8);3482-8. PMID: 3611020

Hidaka90: Hidaka T, Imai S, Hara O, Anzai H, Murakami T, Nagaoka K, Seto H (1990). "Carboxyphosphonoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation." J Bacteriol 172(6);3066-72. PMID: 2160937

Hidaka92: Hidaka T, Hidaka M, Uozumi T, Seto H (1992). "Nucleotide sequence of a carboxyphosphonoenolpyruvate phosphonomutase gene isolated from a bialaphos-producing organism, Streptomyces hygroscopicus, and its expression in Streptomyces lividans." Mol Gen Genet 233(3);476-8. PMID: 1320191

Pollack92: Pollack SJ, Freeman S, Pompliano DL, Knowles JR (1992). "Cloning, overexpression and mechanistic studies of carboxyphosphonoenolpyruvate mutase from Streptomyces hygroscopicus." Eur J Biochem 209(2);735-43. PMID: 1330557


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc11.