|Gene:||Msed_1424||Accession Number: G-10488 (MetaCyc)|
Species: Metallosphaera sedula
Subunit composition of
NADPH-dependent succinate semialdehyde reductase = [Msed_1424]2
succinate semialdehyde reductase monomer = Msed_1424
The activity of this enzyme was first demonstrated in cell extract of Metallosphaera sedula by HPLC [Berg07]. The enzyme was partially purified from an extract of autotrophically grown cells and analyzed by trypsin digestion followed by peptide fingerprint mass spectrometry. The resulting data was compared with the genome, and identified the Msed_1424 gene [Kockelkorn09]. The gene was cloned, expressed in Escherichia coli and purified. The native molecular mass determined by gel filtration was 74 ± 7 kDa, suggesting a homodimeric subunit composition. Metal analysis using plasma emission spectroscopy revealed the presence of 1.9 mol of zinc per mol of enzyme monomer.
The enzyme is very specific for its substrate, and did not use any of the other aldehydes that were tested [Kockelkorn09].
Molecular Weight of Polypeptide: 37.879 kD (from nucleotide sequence), 40.0 kD (experimental) [Kockelkorn09 ]
Molecular Weight of Multimer: 74.0 kD (experimental) [Kockelkorn09]
Relationship Links: InterPro:IN-FAMILY:IPR002085 , InterPro:IN-FAMILY:IPR002328 , InterPro:IN-FAMILY:IPR011032 , InterPro:IN-FAMILY:IPR013149 , InterPro:IN-FAMILY:IPR013154 , InterPro:IN-FAMILY:IPR016040 , Panther:IN-FAMILY:PTHR11695 , Pfam:IN-FAMILY:PF00107 , Pfam:IN-FAMILY:PF08240 , Prosite:IN-FAMILY:PS00059
Enzymatic reaction of: succinate semialdehyde reductase
EC Number: 1.1.1.co
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the opposite direction.
In Pathways: 3-hydroxypropanoate/4-hydroxybutanate cycle
pH(opt): 7.5 [Kockelkorn09]
Kockelkorn09: Kockelkorn D, Fuchs G (2009). "Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales." J Bacteriol 191(20);6352-62. PMID: 19684143
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