MetaCyc Enzyme: isovaleryl-CoA dehydrogenase

Gene: liuA Accession Number: G-12368 (MetaCyc)

Synonyms: PA2015

Species: Pseudomonas aeruginosa PAO1

Subunit composition of isovaleryl-CoA dehydrogenase = [LiuA]4
         isovaleryl-CoA dehydrogenase subunit = LiuA

The native apparent molecular mass was determined by gel filtration chromatography [ForsterFromme08].

This enzyme is a terpenoid-specific, FAD-dependent acyl-CoA dehydrogenase that catalyzes the formation of a carbon-carbon double bond in methyl-branched substrates, similar to citronellyl-CoA dehydrogenase. The gene encoding this enzyme is part of the L-leucine and isovalerate utilizing liuABCDE gene cluster in this organism. A liuA insertion mutant was unable to utilize acyclic terpenes, L-leucine or isovalerate, but could utilize succinate [ForsterFromme08] (see pathways citronellol degradation, cis-genanyl-CoA degradation and L-leucine degradation I).

Recombinant, His-tagged enzyme was expressed in Escherichia coli and purified [ForsterFromme08].
The apparent molecular masss of the polypeptide was determined by SDS-PAGE [ForsterFromme08].

Gene Citations: [Aguilar06, ForsterFromme06]

Map Position: [2,205,190 -> 2,206,353]

Molecular Weight of Polypeptide: 42.209 kD (from nucleotide sequence), 41.0 kD (experimental) [ForsterFromme08 ]

Molecular Weight of Multimer: 163.0 kD (experimental) [ForsterFromme08]

Unification Links: Entrez-gene:879167 , Pride:Q9I296 , Protein Model Portal:Q9I296 , SMR:Q9I296 , String:208964.PA2015 , UniProt:Q9I296

Relationship Links: InterPro:IN-FAMILY:IPR006089 , InterPro:IN-FAMILY:IPR006091 , InterPro:IN-FAMILY:IPR009075 , InterPro:IN-FAMILY:IPR009100 , InterPro:IN-FAMILY:IPR013786 , Pfam:IN-FAMILY:PF00441 , Pfam:IN-FAMILY:PF02770 , Pfam:IN-FAMILY:PF02771 , Prosite:IN-FAMILY:PS00072 , Prosite:IN-FAMILY:PS00073

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 13-Dec-2010 by Fulcher CA , SRI International

Enzymatic reaction of: isovaleryl-CoA dehydrogenase

EC Number:

isovaleryl-CoA + an oxidized electron-transfer flavoprotein + H+ <=> 3-methylcrotonyl-CoA + a reduced electron-transfer flavoprotein

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for isovaleryl-CoA: butanoyl-CoA [ForsterFromme08 ]

In Pathways: L-leucine degradation I

Acyl-CoA dehydrogenase activity was assayed by measuring the reduction of oxidized DCPIP at 600 nm. The enzyme was highly specific for isovaleryl-CoA. It utilized butanoyl-CoA, but with less efficiency. It did not utilize octanoyl-CoA, 5-methylhex-4-enoyl-CoA, (S)-3-hydroxybutanoyl-CoA, isobutanoyl-CoA, or citronellyll-CoA [ForsterFromme08a].

Cofactors or Prosthetic Groups: FAD [ForsterFromme08]

Kinetic Parameters:

Km (μM)


Aguilar06: Aguilar JA, Zavala AN, Diaz-Perez C, Cervantes C, Diaz-Perez AL, Campos-Garcia J (2006). "The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa." Appl Environ Microbiol 72(3);2070-9. PMID: 16517656

ForsterFromme06: Forster-Fromme K, Hoschle B, Mack C, Bott M, Armbruster W, Jendrossek D (2006). "Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa." Appl Environ Microbiol 72(7);4819-28. PMID: 16820476

ForsterFromme08: Forster-Fromme K, Jendrossek D (2008). "Biochemical characterization of isovaleryl-CoA dehydrogenase (LiuA) of Pseudomonas aeruginosa and the importance of liu genes fora functional catabolic pathway of methyl-branched compounds." FEMS Microbiol Lett 286(1);78-84. PMID: 18625020

ForsterFromme08a: Forster-Fromme K, Chattopadhyay A, Jendrossek D (2008). "Biochemical characterization of AtuD from Pseudomonas aeruginosa, the first member of a new subgroup of acyl-CoA dehydrogenases with specificity for citronellyl-CoA." Microbiology 154(Pt 3);789-96. PMID: 18310025

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, biocyc12.