|Gene:||liuA||Accession Number: G-12368 (MetaCyc)|
Species: Pseudomonas aeruginosa PAO1
Subunit composition of
isovaleryl-CoA dehydrogenase = [LiuA]4
isovaleryl-CoA dehydrogenase subunit = LiuA
The native apparent molecular mass was determined by gel filtration chromatography [ForsterFromme08].
This enzyme is a terpenoid-specific, FAD-dependent acyl-CoA dehydrogenase that catalyzes the formation of a carbon-carbon double bond in methyl-branched substrates, similar to citronellyl-CoA dehydrogenase. The gene encoding this enzyme is part of the L-leucine and isovalerate utilizing liuABCDE gene cluster in this organism. A liuA insertion mutant was unable to utilize acyclic terpenes, L-leucine or isovalerate, but could utilize succinate [ForsterFromme08] (see pathways citronellol degradation, cis-genanyl-CoA degradation and L-leucine degradation I).
|Map Position: [2,205,190 -> 2,206,353]|
Molecular Weight of Polypeptide: 42.209 kD (from nucleotide sequence), 41.0 kD (experimental) [ForsterFromme08 ]
Molecular Weight of Multimer: 163.0 kD (experimental) [ForsterFromme08]
Relationship Links: InterPro:IN-FAMILY:IPR006089 , InterPro:IN-FAMILY:IPR006091 , InterPro:IN-FAMILY:IPR009075 , InterPro:IN-FAMILY:IPR009100 , InterPro:IN-FAMILY:IPR013786 , Pfam:IN-FAMILY:PF00441 , Pfam:IN-FAMILY:PF02770 , Pfam:IN-FAMILY:PF02771 , Prosite:IN-FAMILY:PS00072 , Prosite:IN-FAMILY:PS00073
Enzymatic reaction of: isovaleryl-CoA dehydrogenase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: L-leucine degradation I
Acyl-CoA dehydrogenase activity was assayed by measuring the reduction of oxidized DCPIP at 600 nm. The enzyme was highly specific for isovaleryl-CoA. It utilized butanoyl-CoA, but with less efficiency. It did not utilize octanoyl-CoA, 5-methylhex-4-enoyl-CoA, (S)-3-hydroxybutanoyl-CoA, isobutanoyl-CoA, or citronellyll-CoA [ForsterFromme08a].
Aguilar06: Aguilar JA, Zavala AN, Diaz-Perez C, Cervantes C, Diaz-Perez AL, Campos-Garcia J (2006). "The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa." Appl Environ Microbiol 72(3);2070-9. PMID: 16517656
ForsterFromme06: Forster-Fromme K, Hoschle B, Mack C, Bott M, Armbruster W, Jendrossek D (2006). "Identification of genes and proteins necessary for catabolism of acyclic terpenes and leucine/isovalerate in Pseudomonas aeruginosa." Appl Environ Microbiol 72(7);4819-28. PMID: 16820476
ForsterFromme08: Forster-Fromme K, Jendrossek D (2008). "Biochemical characterization of isovaleryl-CoA dehydrogenase (LiuA) of Pseudomonas aeruginosa and the importance of liu genes fora functional catabolic pathway of methyl-branched compounds." FEMS Microbiol Lett 286(1);78-84. PMID: 18625020
ForsterFromme08a: Forster-Fromme K, Chattopadhyay A, Jendrossek D (2008). "Biochemical characterization of AtuD from Pseudomonas aeruginosa, the first member of a new subgroup of acyl-CoA dehydrogenases with specificity for citronellyl-CoA." Microbiology 154(Pt 3);789-96. PMID: 18310025
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