|Gene:||oxdC||Accession Number: BSU33240|
Species: Bacillus subtilis
Subunit composition of
oxalate decarboxylase = [OxdC]6
oxalate decarboxylase monomer = OxdC
Bacillus subtilis expresses a cytosolic oxalate decarboxylase that induced in acidic growth media, particularly at pH 5.0, but not by oxalate. The enzyme was purified, and N-terminal sequencing identified the protein to be encoded by the oxdC gene [Tanner00]. The enzyme preferred acidic conditions, and its pH activity profile yielded a bell-shaped curve with 70% activity at pH 3.0, a maximum at pH 5.0, and zero activity at pH 7.5.
A mechanism for oxalate decarboxylation has been proposed, in which both Mn(II) and O2 are cofactors that act together as a two-electron sink during catalysis [Tanner01].
The structure of the enzyme has been determined at 1.75 Å resolution. The enzyme is a hexamer with 32-point symmetry in which each monomer belongs to the cupin family of proteins. It is classified as a bicupin because it contains two cupin folds. Each oxalate decarboxylase cupin domain contains one manganese binding site, and is structurally similar to oxalate oxidase, an enzyme that catalyzes manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide [Anand02].
|Map Position: [3,410,466 -> 3,411,623]|
Molecular Weight of Polypeptide: 43.566 kD (from nucleotide sequence), 44.0 kD (experimental) [Tanner00 ]
Molecular Weight of Multimer: 220.0 kD (experimental) [Tanner00]
pI: 6.1 [Tanner00]
Unification Links: Entrez:2635821 , GenoList (SubtiList):BSU33240 , GOA:O34714 , Protein Model Portal:O34714 , SMR:O34714 , String:224308.BSU33240 , String:BSU33240 , SubtilisWiki:oxdC , SubtiWiki:oxdC , UniProt:O34714
Relationship Links: InterPro:IN-FAMILY:IPR006045 , InterPro:IN-FAMILY:IPR011051 , InterPro:IN-FAMILY:IPR014710 , InterPro:IN-FAMILY:IPR017774 , PDB:Structure:1J58 , PDB:Structure:1L3J , PDB:Structure:1UW8 , PDB:Structure:2UY8 , PDB:Structure:2UY9 , PDB:Structure:2UYA , PDB:Structure:2UYB , PDB:Structure:2V09 , PDB:Structure:3S0M , PDB:Structure:4MET , Pfam:IN-FAMILY:PF00190 , Smart:IN-FAMILY:SM00835
Enzymatic reaction of: oxalate decarboxylase
EC Number: 220.127.116.11
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
Reversibility of this reaction is unspecified.
In Pathways: oxalate degradation V
pH(opt): 5 [Tanner00]
Tanner01: Tanner A, Bowater L, Fairhurst SA, Bornemann S (2001). "Oxalate decarboxylase requires manganese and dioxygen for activity. Overexpression and characterization of Bacillus subtilis YvrK and YoaN." J Biol Chem 276(47);43627-34. PMID: 11546787
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