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MetaCyc Enzyme: DNA-specific endonuclease I

Gene: endA Accession Numbers: EG11336 (MetaCyc), b2945, ECK2940

Species: Escherichia coli K-12 substr. MG1655

Summary:
Endonuclease I is a periplasmic enzyme that cleaves within duplex DNA. Its cellular role is unknown, but it has seen wide use as an experimental tool for DNA footprinting.

Endonuclease I cleaves within duplex DNA no shorter than 7 bp [Lehman62]. It is inhibited by tRNA and rRNA [Lehman62a].

Mutants lacking endA show no apparent different from wild type cells [Durwald68].

Citations: [Ehrlich73a, Bernardi65]

Locations: periplasmic space

Map Position: [3,088,369 -> 3,089,076]

Molecular Weight of Polypeptide: 26.709 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009661 , CGSC:824 , EchoBASE:EB1312 , EcoGene:EG11336 , EcoliWiki:b2945 , ModBase:P25736 , OU-Microarray:b2945 , PortEco:endA , PR:PRO_000022515 , Protein Model Portal:P25736 , RefSeq:NP_417420 , RegulonDB:EG11336 , SMR:P25736 , String:511145.b2945 , Swiss-Model:P25736 , UniProt:P25736

Relationship Links: InterPro:IN-FAMILY:IPR007346 , Pfam:IN-FAMILY:PF04231

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0000737 - DNA catabolic process, endonucleolytic Author statement [Lehman62]
Molecular Function: GO:0004519 - endonuclease activity Inferred from experiment Inferred by computational analysis [UniProtGOA11, Lehman62]
GO:0004518 - nuclease activity Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0004530 - deoxyribonuclease I activity Inferred by computational analysis [GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [DiazMejia09, Cordonnier65]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]

MultiFun Terms: information transfer DNA related DNA degradation
metabolism degradation of macromolecules DNA

Credits:
Revised in EcoCyc 25-May-2011 by Weerasinghe D
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Enzymatic reaction of: deoxyribonuclease I (DNA-specific endonuclease I)

EC Number: 3.1.21.1

a double stranded DNA[periplasmic space] + 2n H2O[periplasmic space] <=> n a 5'-phosphooligonucleotide[periplasmic space] + n a 5' phosphodinucleotide[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International

Cofactors or Prosthetic Groups: Mg2+ [Lehman62]

pH(opt): 7.5-8.5 [Lehman62]


Sequence Features

Feature Class Location Citations Comment
Signal-Sequence 1 -> 22
[UniProt10]
UniProt: Non-Experimental Qualifier: potential;
Chain 23 -> 235
[UniProt09]
UniProt: Endonuclease-1;

History:
10/20/97 Gene b2945 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11336; confirmed by SwissProt match.


References

Bernardi65: Bernardi G, Cordonnier C (1965). "Mechanism of degradation of DNA by endonuclease I from Escherichia coli." J Mol Biol 11;141-3. PMID: 14255756

Cordonnier65: Cordonnier C, Bernardi G (1965). "Localization of E. coli endonuclease I." Biochem Biophys Res Commun 20(5);555-9. PMID: 4955462

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Durwald68: Durwald H, Hoffmann-Berling H (1968). "Endonuclease-I-deficient and ribonuclease I-deficient Escherichia coli mutants." J Mol Biol 34(2);331-46. PMID: 4938550

Ehrlich73a: Ehrlich SD, Bertazzoni U, Bernardi G (1973). "The specificity of Escherichia coli endonuclease I." Eur J Biochem 40(1);149-53. PMID: 4589547

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Lehman62: Lehman IR, Roussos GG, Pratt EA (1962). "The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease." J Biol Chem 237;819-28. PMID: 14463751

Lehman62a: Lehman IR, Roussos GG, Pratt EA (1962). "The deoxyribo-nucleases of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid." J Biol Chem 237;829-33. PMID: 14463750

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, BIOCYC14A.