MetaCyc Enzyme: mannokinase

Species: Escherichia coli K-12 substr. MG1655

Mannokinase activity in E. coli is constitutively expressed; the enzyme can also phosphorylate fructose and to a lesser extent glucosamine, D-glucose and 2-deoxyglucose [Sebastian67, Sebastian72].

The gene encoding mannokinase is unknown.

Gene-Reaction Schematic

Gene-Reaction Schematic

Imported from EcoCyc 15-Mar-2016 by Paley S, SRI International

Enzymatic reaction of: mannokinase

Inferred from experiment

Synonyms: ATP:D-mannose 6-phosphotransferase

EC Number:

D-mannopyranose + ATP → D-mannopyranose 6-phosphate + ADP + H+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for D-mannopyranose: D-fructose [Sebastian67, Sebastian72]
Alternative Substrates for ATP: ITP [Sebastian72]

Imported from EcoCyc 15-Mar-2016 by Paley S, SRI International

Cofactors or Prosthetic Groups: Mg2+ [Sebastian72]

Inhibitors (Competitive): D-mannosamine [Sebastian72]Kinetic Parameters:
Substrate Km (μM) Citations
ATP 200.0 [Sebastian72]
D-mannopyranose 200.0 [Sebastian72]

pH(opt): 7.5-8.5 [Sebastian72]


Sebastian67: Sebastian J, Asensio C (1967). "Identification of mannokinase in Escherichia coli." Biochem Biophys Res Commun 28(2);197-202. PMID: 5342371

Sebastian72: Sebastian J, Asensio C (1972). "Purification and properties of the mannokinase from Escherichia coli." Arch Biochem Biophys 151(1);227-33. PMID: 4557975

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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