Species: Escherichia coli K-12 substr. MG1655
Methylglyoxal reductase catalyzes the conversion of methylglyoxal into lactaldehyde. The lactaldehyde is then acted upon by lactaldehyde dehydrogenase yielding lactate. This provides an alternate route for methylglyoxal metabolism [Saikusa87]. No gene has been identified for this enzyme.
Molecular Weight of Polypeptide: 43.0 kD (experimental) [Saikusa87]
Enzymatic reaction of: methylglyoxal reductase (NADPH-dependent)
EC Number: 18.104.22.1683(S)-lactaldehyde + NADP+ ← methylglyoxal + NADPH + H+
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
The reaction is favored in the opposite direction.Alternative Substrates for methylglyoxal [Saikusa87]: glycolaldehyde [Saikusa87], acetaldehyde [Saikusa87], propanal [Saikusa87], DL-glyceraldehyde [Saikusa87], 4,5-dioxopentanoate [Saikusa87], phenylglyoxal [Saikusa87], glyoxal [Saikusa87]
The enzyme was purified from E. coli IFO 3806 [Saikusa87].
T(opt): 45 °C [Saikusa87]
pH(opt): 6.5 [Saikusa87]
Saikusa87: Saikusa T, Rhee H, Watanabe K, Murata K, Kimura A (1987). "Metabolism of 2-oxoaldehydes in bacteria: purification and characterization of methylglyoxal reductase from E. coli." Agricultural and Biological Chemistry 51(7): 1893-1899.
©2016 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493