Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: ferrochelatase

Gene: hemH Accession Number: G-9035 (MetaCyc)

Species: Rhodobacter sphaeroides

Summary:
The molecular weight was determined by SDS-PAGE. Gel filtration chromatography of solubilized ferrochelatase in the presence of 0.5% sodium deoxycholate gave a molecular weight of 110 kD. Contrary to a previous report, the calculated millimolar extinction coefficient at 278 nm was 76, based upon a molecular weight of 115 kD [Dailey86].

Locations: membrane

Map Position: [2,967,945 -> 2,969,078]

Molecular Weight of Polypeptide: 115 kD (experimental) [Dailey86 ]

Unification Links: Entrez-gene:3718861

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0016020 - membrane [Dailey86]


Enzymatic reaction of: ferrochelatase

EC Number: 4.99.1.1

protoporphyrin IX[periplasmic space] + Fe2+[periplasmic space] <=> ferroheme b[periplasmic space] + 2 H+[periplasmic space]

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of heme biosynthesis from glycine , heme biosynthesis I (aerobic)

Summary:
Ferrochelatase catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme (heme) [Dailey86].

Inhibitors (Unknown Mechanism): Hg2+ [Dailey86] , N-ethylmaleimide [Dailey86] , iodoacetamide [Dailey86] , copper sulfate [Dailey86] , diacetyl [Dailey86] , heme b [Dailey86]

Kinetic Parameters:

Substrate
Km (μM)
Citations
Fe2+
22.0
[Dailey86]
protoporphyrin IX
18.0
[Dailey86]


References

Dailey86: Dailey HA (1986). "Purification and characterization of bacterial ferrochelatase." Methods Enzymol 123;408-15. PMID: 3702737


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13A.