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MetaCyc Enzyme: raffinose synthase

Synonyms: galactinol:sucrose 6-galactosyltransferase

Species: Vicia faba

Summary:
A raffinose synthase (RFS) was purified 400-fold from maturing seeds of Vicia faba [Lehle73]. The protein was shown to catalyze the transfer of the galactosyl moiety of galactinol to sucrose, leading to the formation of raffinose and myo-inositol. The chemical properties, inhibitors and substrate specificity of the enzyme were determined.

Molecular Weight of Polypeptide: 80 kD (experimental) [Lehle73 ]

Gene-Reaction Schematic: ?

Gene-Reaction Schematic


Enzymatic reaction of: raffinose synthase

EC Number: 2.4.1.82

galactinol + sucrose <=> myo-inositol + raffinose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: stachyose biosynthesis

Inhibitors (Other): Ag+ [Lehle73] , Hg2+ [Lehle73] , Zn2+ [Lehle73] , Al3+ [Lehle73] , Mn2+ [Lehle73]

Kinetic Parameters:

Substrate
Km (μM)
Citations
galactinol
1000.0
[Lehle73]
raffinose
10000.0
[Lehle73]
sucrose
2900.0
[Lehle73]

T(opt): 42 °C [Lehle73]

pH(opt): 7.0 [Lehle73]


References

Lehle73: Lehle L, Tanner W (1973). "The function of myo-inositol in the biosynthesis of raffinose. Purification and characterization of galactinol:sucrose 6-galactosyltransferase from Vicia faba seeds." Eur J Biochem 38(1);103-10. PMID: 4774118


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Sat May 23, 2015, biocyc14.