Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: raffinose synthase

Synonyms: galactinol:sucrose 6-galactosyltransferase

Species: Vicia faba

Summary:
A raffinose synthase (RFS) was purified 400-fold from maturing seeds of Vicia faba [Lehle73]. The protein was shown to catalyze the transfer of the galactosyl moiety of galactinol to sucrose, leading to the formation of raffinose and myo-inositol. The chemical properties, inhibitors and substrate specificity of the enzyme were determined.

Molecular Weight of Polypeptide: 80 kD (experimental) [Lehle73 ]

Gene-Reaction Schematic: ?


Enzymatic reaction of: raffinose synthase

EC Number: 2.4.1.82

galactinol + sucrose <=> myo-inositol + raffinose

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: stachyose biosynthesis

Inhibitors (Other): Ag+ [Lehle73] , Hg2+ [Lehle73] , Zn2+ [Lehle73] , Al3+ [Lehle73] , Mn2+ [Lehle73]

Kinetic Parameters:

Substrate
Km (μM)
Citations
galactinol
1000.0
[Lehle73]
raffinose
10000.0
[Lehle73]
sucrose
2900.0
[Lehle73]

T(opt): 42 °C [Lehle73]

pH(opt): 7.0 [Lehle73]


References

Lehle73: Lehle L, Tanner W (1973). "The function of myo-inositol in the biosynthesis of raffinose. Purification and characterization of galactinol:sucrose 6-galactosyltransferase from Vicia faba seeds." Eur J Biochem 38(1);103-10. PMID: 4774118


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, BIOCYC14B.