Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: 2-keto-6-phosphogluconate reductase

Species: Pseudomonas fluorescens

Summary:
The subunit structure of this enzyme has not been reported.

Unification Links: Entrez:AAY91990

Gene-Reaction Schematic: ?

Credits:
Created 01-Dec-2006 by Fulcher CA , SRI International


Enzymatic reaction of: 2-keto-6-phosphogluconate reductase

EC Number: 1.1.1.43

D-gluconate 6-phosphate + NAD(P)+ <=> 6-phospho-2-dehydro-D-gluconate + NAD(P)H + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

Alternative Substrates for 6-phospho-2-dehydro-D-gluconate: 2-keto-D-gluconate [Frampton61 ]

In Pathways: glucose degradation (oxidative)

Summary:
The enzyme can use both NADPH and NADH. The Km for 6-phospho-2-dehydro-D-gluconate (2-keto-6-phosphogluconate) with NADPH as hydrogen donor was 380 μM, and with NADH was 660 μM. The average Km for NADPH was approximately 20 times lower than for NADH, suggesting that it is the more active hydrogen donor. With NADPH as hydrogen donor, the activity was inhibited by 10 mM p-chloromercuribenzoate. [Frampton61]

Inhibitors (Unknown Mechanism): p-chloromercuribenzoate [Frampton61]

pH(opt): 7.4 [Frampton61]


References

Frampton61: Frampton EW, Wood WA (1961). "Carbohydrate oxidation by Pseudomonas fluorescens VI. Conversion of 2-keto-6-phosphogluconate to pyruvate." J Biol Chem 236;2571-7. PMID: 13894458


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14A.