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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
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MetaCyc Enzyme: methylglyoxal reductase (NADH-dependent)

Species: Rattus norvegicus

Summary:
An enzyme has been partially purified from rat liver that oxidizes (R)-lactaldehyde to methylglyoxal using NAD+ as the elctrons acceptor [Ting65]. The enzyme is specific to the D-isomer of lactaldehyde and to NADH, and catalyzes a reversible reaction. Enzymatic activity was found in all tissues tested (muscle, brain, kidney, heart, liver, spleen, lung), but was strongest in skeletal muscle and weakest in lung.

This mammalian enzyme differs from methylglyoxal reductase (NADPH-dependent) (EC 1.1.1.283) in using NADH rather than NADPH. In addition, 1.1.1.283 catalyzes a unidirectional reduction of methylglyoxal to (R)-lactaldehyde, while the mammalian enzyme catalyzes a reversible reaction.

Gene-Reaction Schematic: ?

Credits:
Created 16-Jan-2007 by Caspi R , SRI International


Enzymatic reaction of: methylglyoxal reductase (NADH-dependent)

EC Number: 1.1.1.78

(R)-lactaldehyde + NAD+ <=> methylglyoxal + NADH + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for (R)-lactaldehyde: D-glyceraldehyde [Ting65 ]

In Pathways: methylglyoxal degradation VI

Inhibitors (Other): p-chloromercuribenzoate [Ting65] , iodoacetate [Ting65] , N-ethylmaleimide [Ting65]

Kinetic Parameters:

Substrate
Km (μM)
Citations
NAD+
34.0
[Ting65]
(R)-lactaldehyde
130.0
[Ting65]

pH(opt): 11 [Ting65]


References

Ting65: Ting, S.M., Miller, O.N., Sellinger, O.Z. (1965). "The metabolism of lactaldehyde. VII. The oxidation of D-lactaldehyde in rat liver." Biochim Biophys Acta 97;407-15. PMID: 14323585


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, BIOCYC13A.