Species: Rattus norvegicus
An enzyme has been partially purified from rat liver that oxidizes (R)-lactaldehyde to methylglyoxal using NAD+ as the elctrons acceptor [Ting65]. The enzyme is specific to the D-isomer of lactaldehyde and to NADH, and catalyzes a reversible reaction. Enzymatic activity was found in all tissues tested (muscle, brain, kidney, heart, liver, spleen, lung), but was strongest in skeletal muscle and weakest in lung.
This mammalian enzyme differs from methylglyoxal reductase (NADPH-dependent) (EC 184.108.40.2063) in using NADH rather than NADPH. In addition, 220.127.116.113 catalyzes a unidirectional reduction of methylglyoxal to (R)-lactaldehyde, while the mammalian enzyme catalyzes a reversible reaction.
Enzymatic reaction of: methylglyoxal reductase (NADH-dependent)
EC Number: 18.104.22.168(R)-lactaldehyde + NAD+ ⇄ methylglyoxal + NADH + H+
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.
This reaction is reversible.Alternative Substrates for (R)-lactaldehyde: D-glyceraldehyde [Ting65]
In Pathways: methylglyoxal degradation VIInhibitors (Other): p-chloromercuribenzoate [Ting65], iodoacetate [Ting65], N-ethylmaleimide [Ting65]Kinetic Parameters:
pH(opt): 11 [Ting65]
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