|Gene:||pno||Accession Number: G-10000 (MetaCyc)|
Species: Mesorhizobium loti
Sequence analysis has shown that the pno gene of Mesorhizobium loti is similar to genes encoding pyridoxine 4-oxidase in other organisms. The gene was cloned and expressed in Escherichia coli, and found to encode an active monomeric pyridoxine 4-oxidase. This led to the finding that Mesorhizobium loti posseses a degradation pathway for pyridoxamine and pyridoxine, and is able to utilize these compounds as sole carbon and nitrogen sources.
Enzymatic activity was also measured in crude extracts of Mesorhizobium loti grown on Rhizobium medium supplemented with pyridoxine (0.63 mU/mg), demonstrating that the enzyme is expressed in its native host [Yuan04].
The cloned gene was later expressed in E. coli as a His-tagged protein, and purified to homogeneity. The purified enzyme was crystallized [Mugo12].
Gene Citations: [Kaneko00]
Molecular Weight of Polypeptide: 55.712 kD (from nucleotide sequence), 54.5 kD (experimental) [Yuan04 ]
Relationship Links: Entrez-Nucleotide:RELATED-TO:BA000012 , InterPro:IN-FAMILY:IPR000172 , InterPro:IN-FAMILY:IPR007867 , InterPro:IN-FAMILY:IPR012132 , Pfam:IN-FAMILY:PF00732 , Pfam:IN-FAMILY:PF05199 , Prosite:IN-FAMILY:PS00623 , Prosite:IN-FAMILY:PS00624
Enzymatic reaction of: pyridoxine 4-oxidase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
In Pathways: vitamin B6 degradation
T(opt): 40 °C [Yuan04]
pH(opt): 8-8.5 [Yuan04]
Kaneko00: Kaneko T, Nakamura Y, Sato S, Asamizu E, Kato T, Sasamoto S, Watanabe A, Idesawa K, Ishikawa A, Kawashima K, Kimura T, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Mochizuki Y, Nakayama S, Nakazaki N, Shimpo S, Sugimoto M, Takeuchi C, Yamada M, Tabata S (2000). "Complete genome structure of the nitrogen-fixing symbiotic bacterium Mesorhizobium loti." DNA Res 7(6);331-8. PMID: 11214968
Mugo12: Mugo AN, Kobayashi J, Mikami B, Ohnishi K, Yagi T (2012). "Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase, the first enzyme in pyridoxine degradation pathway I." Acta Crystallogr Sect F Struct Biol Cryst Commun 68(Pt 1);66-8. PMID: 22232175
Yuan04: Yuan B, Yoshikane Y, Yokochi N, Ohnishi K, Yagi T (2004). "The nitrogen-fixing symbiotic bacterium Mesorhizobium loti has and expresses the gene encoding pyridoxine 4-oxidase involved in the degradation of vitamin B6." FEMS Microbiol Lett 234(2);225-30. PMID: 15135526
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