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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: 2-methyl-3-hydroxypyridine-5-carboxylate oxygenase

Species: Pseudomonas sp. MA-1

Summary:
2-methyl-3-hydroxypyridine-5-carboxylate oxygenase has been purified from Pseudomonas sp. MA-1 and characterized [Sparrow69, Burg70]. The enzyme contains 2 moles of FAD per mole protein, and FMN cannot substitute [Sparrow69]. It is also highly specific for 3-hydroxy-2-methylpyridine-5-carboxylate, and molecular oxygen cannot be replaced by oxidized DCPIP. The enzyme is sensitive to oxidation, and loses activity reapidly in the absence of a reducing agent such as mercaptopethanol. Both NADH and NADPH can be used for reduction of the FAD-enzyme complex [Sparrow69].

Molecular Weight of Polypeptide: 166 kD (experimental) [Sparrow69 ]

Gene-Reaction Schematic: ?

Credits:
Created 05-Apr-2007 by Caspi R , SRI International


Enzymatic reaction of: 2-methyl-3-hydroxypyridine-5-carboxylate oxygenase

Synonyms: 3-hydroxy-2-methylpyridine-5-carboxylate oxygenase

EC Number: 1.14.12.4

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + oxygen <=> 2-(acetamidomethylene)succinate + NAD(P)+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for 3-hydroxy-2-methylpyridine-5-carboxylate: 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate [Sparrow69 ]

In Pathways: vitamin B6 degradation

Cofactors or Prosthetic Groups: FAD [Burg70]

Inhibitors (Competitive): 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate [Burg70] , 6-methylnicotinate [Burg70]

Inhibitors (Unknown Mechanism): p-chloromercuribenzoate [Burg70]

Kinetic Parameters:

Substrate
Km (μM)
Citations
NAD(P)H
100.0
[Burg70]
3-hydroxy-2-methylpyridine-5-carboxylate
48.0
[Burg70]

pH(opt): 6.5-8 [Sparrow69]


References

Burg70: Burg, R.W. (1970). "The catabolism of vitamin B6." Methods Enzymol. 18(1): 634-658.

Sparrow69: Sparrow LG, Ho PP, Sundaram TK, Zach D, Nyns EJ, Snell EE (1969). "The bacterial oxidation of vitamin B6. VII. Purification, properties, and mechanism of action of an oxygenase which cleaves the 3-hydroxypyridine ring." J Biol Chem 244(10);2590-600. PMID: 4306031


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, BIOCYC13A.