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MetaCyc Enzyme: 2-methyl-3-hydroxypyridine-5-carboxylate oxygenase

Species: Pseudomonas sp. MA-1

2-methyl-3-hydroxypyridine-5-carboxylate oxygenase has been purified from Pseudomonas sp. MA-1 and characterized [Sparrow69, Burg70]. The enzyme contains 2 moles of FAD per mole protein, and FMN cannot substitute [Sparrow69]. It is also highly specific for 3-hydroxy-2-methylpyridine-5-carboxylate, and molecular oxygen cannot be replaced by oxidized DCPIP. The enzyme is sensitive to oxidation, and loses activity reapidly in the absence of a reducing agent such as mercaptopethanol. Both NADH and NADPH can be used for reduction of the FAD-enzyme complex [Sparrow69].

Molecular Weight of Polypeptide: 166 kD (experimental) [Sparrow69]

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 05-Apr-2007 by Caspi R, SRI International

Enzymatic reaction of: 2-methyl-3-hydroxypyridine-5-carboxylate oxygenase

Inferred from experiment

Synonyms: 3-hydroxy-2-methylpyridine-5-carboxylate oxygenase

EC Number:

3-hydroxy-2-methylpyridine-5-carboxylate + NAD(P)H + oxygen → 2-(acetamidomethylene)succinate + NAD(P)+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for 3-hydroxy-2-methylpyridine-5-carboxylate: 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate [Sparrow69]

In Pathways: vitamin B6 degradation

Cofactors or Prosthetic Groups: FAD [Burg70]

Inhibitors (Competitive): 3-hydroxy-4-hydroxymethyl-2-methylpyridine-5-carboxylate [Burg70], 6-methylnicotinate [Burg70] Inhibitors (Unknown Mechanism): p-chloromercuribenzoate [Burg70]Kinetic Parameters:
Substrate Km (μM) Citations
NAD(P)H 100.0 [Burg70]
3-hydroxy-2-methylpyridine-5-carboxylate 48.0 [Burg70]

pH(opt): 6.5-8 [Sparrow69]


Burg70: Burg, R.W. (1970). "The catabolism of vitamin B6." Methods Enzymol. 18(1): 634-658.

Sparrow69: Sparrow LG, Ho PP, Sundaram TK, Zach D, Nyns EJ, Snell EE (1969). "The bacterial oxidation of vitamin B6. VII. Purification, properties, and mechanism of action of an oxygenase which cleaves the 3-hydroxypyridine ring." J Biol Chem 244(10);2590-600. PMID: 4306031

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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