MetaCyc Enzyme: L-sorbose dehydrogenase, FAD dependent

Gene: BAA13144 Accession Number: G-10471 (MetaCyc)

Species: Gluconobacter oxydans UV10

The membrane-bound L-sorbose dehydrogenase, FAD dependent catalyzes the oxidation of L-sorbopyranose to L-sorbosone.

The enzyme from Gluconobacter oxydans UV10 (derived from strain IFO 3239) has been purified, and found to contain a flavin cofactor, presumably FAD [Sugisawa91].

The gene encoding the enzyme has been cloned and sequenced, and expressed in Escherichia coli K-12. No formal name has been suggested for the gene [Saito97a].

Locations: inner membrane

Molecular Weight of Polypeptide: 57.624 kD (from nucleotide sequence), 58.0 kD (experimental) [Sugisawa91 ]

Unification Links: Protein Model Portal:Q47944 , UniProt:Q47944

Relationship Links: Entrez-Nucleotide:RELATED-TO:D86622 , InterPro:IN-FAMILY:IPR000172 , InterPro:IN-FAMILY:IPR007867 , InterPro:IN-FAMILY:IPR012132 , Pfam:IN-FAMILY:PF00732 , Pfam:IN-FAMILY:PF05199 , Prosite:IN-FAMILY:PS00623 , Prosite:IN-FAMILY:PS00624

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Cellular Component: GO:0005886 - plasma membrane [Sugisawa91]

Created 05-Feb-2008 by Caspi R , SRI International

Enzymatic reaction of: L-sorbose dehydrogenase

EC Number:

keto-L-sorbose + an oxidized unknown electron acceptor <=> L-sorbosone + an reduced unknown electron acceptor

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: 2-keto-L-gulonate biosynthesis

Cofactors or Prosthetic Groups: FAD [Sugisawa91]

Activators (Allosteric): Fe3+ [Sugisawa91] , Co2+ [Sugisawa91]

Inhibitors (Other): quinine [Sugisawa91] , iodoacetate [Sugisawa91]

Inhibitors (Unknown Mechanism): Cu2+ [Sugisawa91]

T(opt): 48 °C [Sugisawa91]

pH(opt): 7 [Sugisawa91]


Saito97a: Saito Y, Ishii Y, Hayashi H, Imao Y, Akashi T, Yoshikawa K, Noguchi Y, Soeda S, Yoshida M, Niwa M, Hosoda J, Shimomura K (1997). "Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-L-gulonate, a precursor of L-ascorbic acid, in a recombinant G. oxydans strain." Appl Environ Microbiol 63(2);454-60. PMID: 9023923

Sugisawa91: Sugisawa, T., Hoshino, T., Nomura, S., Fujiwara, A. (1991). "Isolation and characterization of membrane-bound L-sorbose dehydrogenase from Gluconobacter melanogenus UV10." Agric. Biol. Chem. 55(2): 363-370.

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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