|Gene:||NCS1||Accession Number: G-10573 (MetaCyc)|
Species: Coptis japonica dissecta
CjNCS1 encodes the major norcoclaurine synthase in Coptis japonica. The enzyme catalyzes stereo-specific formation of (S)-norcoclaurine from dopamine and 4-hydroxyphenylacetaldehyde, and is Fe2+-dependent. Among the amines tested, CjNCS1 only accepted dopamine, but not tyramine and tryptamine. On the other hand, besides 4-hydroxyphenylacetaldehyde, the enzyme also reacted with phenylacetaldehyde and 3,4-dihydroxyphenylacetaldehyde.
The sequence of CjNCS1 shares no homology to the NCS gene in T. flavum. The T. flavum NCS homolog in C. japonica, CjPR10A, also has norcoclaurine synthase activity in vitro. It may represent a minor norcoclaurine synthase in vivo. CjPR10A also catalyzes the condensation of dopamine with 4-hydroxyphenylpyruvate.
Molecular Weight of Polypeptide: 40.0 kD (experimental) [Minami07 ]
Relationship Links: Entrez-Nucleotide:PART-OF:AB267398
|Cellular Component:||GO:0005829 - cytosol [Minami07]|
Enzymatic reaction of: norcoclaurine synthase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the direction shown.
In Pathways: (S)-reticuline biosynthesis I
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