MetaCyc Enzyme: 4-hydroxybenzoate-polyprenyltransferase

Gene: ubiA Accession Number: G-10583 (MetaCyc)

Species: Pseudomonas putida

4-hydroxybenzoate-polyprenyltransferase was partially purified from Pseudomonas putida by ion-exchange and gel filtration column chromatography [Kawahara91]. The enzyme required phospholipid as an essential factor for activity. a 1-acyl 2-lyso-phosphatidylethanolamine was particularly potent activator. all-trans-hexaprenyl diphosphate and geranylfarnesyl diphosphate were suitable substitutes for all-trans-nonaprenyl diphosphate as polyprenyl donors.

No inhibition was observed by the end-product, ubiquinone-9. Long chain acyl-CoA, free fatty acids, or isopentenyl diphosphate strongly inhibited the enzyme activity.

Gene Citations: [Nelson02]

Molecular Weight of Polypeptide: 32.936 kD (from nucleotide sequence)

Unification Links: Entrez-gene:1042212 , String:160488.PP_5318 , UniProt:Q88C65

Relationship Links: InterPro:IN-FAMILY:IPR000537 , InterPro:IN-FAMILY:IPR006370 , Pfam:IN-FAMILY:PF01040 , Prosite:IN-FAMILY:PS00943

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Created 01-Apr-2008 by Caspi R , SRI International

Enzymatic reaction of: 4-hydroxybenzoate-polyprenyltransferase

all-trans-nonaprenyl diphosphate + 4-hydroxybenzoate <=> 3-nonaprenyl-4-hydroxybenzoate + diphosphate

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

Alternative Substrates for all-trans-nonaprenyl diphosphate: all-trans-hexaprenyl diphosphate [Kawahara91 ] , geranylfarnesyl diphosphate [Kawahara91 ]

In Pathways: ubiquinol-9 biosynthesis (prokaryotic)

Activators (Allosteric): a 1-acyl 2-lyso-phosphatidylethanolamine [Kawahara91]

Inhibitors (Unknown Mechanism): a long-chain acyl-CoA [Kawahara91] , a fatty acid [Kawahara91] , isopentenyl diphosphate [Kawahara91]


Kawahara91: Kawahara, K., Koizumi, N., Kawaji, H., Oishi, K., Uchida, K. (1991). "Partial Purification and Characterization of 4-Hydroxybenzoate-polyprenyltransferase in Ubiquinone Biosynthesis of Pseudomonas putida." Agricultural and biological chemistry 55(9):2307-2311.

Nelson02: Nelson KE, Weinel C, Paulsen IT, Dodson RJ, Hilbert H, Martins dos Santos VA, Fouts DE, Gill SR, Pop M, Holmes M, Brinkac L, Beanan M, DeBoy RT, Daugherty S, Kolonay J, Madupu R, Nelson W, White O, Peterson J, Khouri H, Hance I, Chris Lee P, Holtzapple E, Scanlan D, Tran K, Moazzez A, Utterback T, Rizzo M, Lee K, Kosack D, Moestl D, Wedler H, Lauber J, Stjepandic D, Hoheisel J, Straetz M, Heim S, Kiewitz C, Eisen JA, Timmis KN, Dusterhoft A, Tummler B, Fraser CM (2002). "Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Environ Microbiol 4(12);799-808. PMID: 12534463

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.0 on Thu Jul 30, 2015, biocyc12.