|Gene:||txyA||Accession Number: G-12787 (MetaCyc)|
Species: Vibrio sp. XY-214
(1,3)-β-Xylan is found mainly in the cell walls of red and green algae, and is a homopolysaccharide.
A endo-1,3-β-xylanase has been purified to homogeneity from the marine bacterium Vibrio sp. XY-214. The purified enzyme hydrolyzed β-1,3-xylan to produce mainly (1,3)-β-xylotriose and (1,3)-β-xylobiose [Araki99]. It did not act on (1,3)-β-xylobiose, p-nitrophenyl-β-D-xylopyranoside, a (1→4)-β-D-xylan, a 1,3-β-D-glucan, or carboxymethyl cellulose.
The N-terminal amino acid sequence of the purified enzyme was determined and used for cloning the gene by PCR. The txyA gene was expressed in Escherichia coli, And the recombinant protein was purified and characterized as well [Araki00].
The gene consists of a signal peptide of 22 amino acid residues, followed by a catalytic domain which belongs to family 26 of the glycosyl hydrolases, a linker region with one array of DGG and six repeats of DNGG, and a carbohydrate-binding module (CBM31) that binds pecifically to β-1,3-xylan [Okazaki02].
Locations: extracellular space
Molecular Weight of Polypeptide: 51.324 kD (from nucleotide sequence), 52.0 kD (experimental) [Araki99 ]
pI: 3.6 [Araki99]
|Cellular Component:||GO:0005576 - extracellular region|
Enzymatic reaction of: endo-1,3-β-xylanase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
In Pathways: (1,3)-β-D-xylan degradation
T(opt): 37 °C [Araki99]
pH(opt): 7 [Araki99]
Araki00: Araki T, Hashikawa S, Morishita T (2000). "Cloning, sequencing, and expression in Escherichia coli of the new gene encoding beta-1,3-xylanase from a marine bacterium, Vibrio sp. strain XY-214." Appl Environ Microbiol 66(4);1741-3. PMID: 10742274
Araki99: Araki T, Tani S, Maeda K, Hashikawa S, Nakagawa H, Morishita T (1999). "Purification and characterization of beta-1,3-xylanase from a marine bacterium, Vibrio sp. XY-214." Biosci Biotechnol Biochem 63(11);2017-9. PMID: 10635569
Kiyohara06: Kiyohara M, Hama Y, Yamaguchi K, Ito M (2006). "Structure of beta-1,3-xylooligosaccharides generated from Caulerpa racemosa var. laete-virens beta-1,3-xylan by the action of beta-1,3-xylanase." J Biochem 140(3);369-73. PMID: 16891637
Lahaye03: Lahaye M, Rondeau-Mouro C, Deniaud E, Buleon A (2003). "Solid-state 13C NMR spectroscopy studies of xylans in the cell wall of Palmaria palmata (L. Kuntze, Rhodophyta)." Carbohydr Res 338(15);1559-69. PMID: 12860427
Okazaki02: Okazaki F, Tamaru Y, Hashikawa S, Li YT, Araki T (2002). "Novel carbohydrate-binding module of beta-1,3-xylanase from a marine bacterium, Alcaligenes sp. strain XY-234." J Bacteriol 184(9);2399-403. PMID: 11948152
Yamagaki96: Yamagaki, T., Maeda, M., Kabazawa, K., Ishizuka, Y, Nakanishi, H. (1996). "Structures of Caulerpa cell wall microfibril xylan with detection of ?-1,3-xylooligosaccharides as revealed by matrix-assisted laser desorption ionization/time of flight/mass spectrometry." Biosci. Biotech. Biochem. 60, 1222-1228.
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