|Gene:||vioD||Accession Number: G-15145 (MetaCyc)|
Species: Chromobacterium violaceum
The subunit structure of this enzyme has not been reported.
VioD is a flavin-dependent and NADPH-dependent monooxygenase that catalyzes the hydroxylation of protodeoxyviolaceinate to yield protoviolaceinate in pathway violacein biosynthesis ([Shinoda07] and reviewed in [Hoshino11]).
The crystal structure has been determined, but no publication has resulted from the structure to date.
Gene Citations: [August00]
|Map Position: [3,559,549 <- 3,560,670]|
Molecular Weight of Polypeptide: 41.623 kD (from nucleotide sequence)
Relationship Links: PDB:Structure:3C4A
Enzymatic reaction of: protoviolaceinate synthase
EC Number: 1.14.13.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
In Pathways: violacein biosynthesis
August00: August PR, Grossman TH, Minor C, Draper MP, MacNeil IA, Pemberton JM, Call KM, Holt D, Osburne MS (2000). "Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum." J Mol Microbiol Biotechnol 2(4);513-9. PMID: 11075927
Hoshino11: Hoshino T (2011). "Violacein and related tryptophan metabolites produced by Chromobacterium violaceum: biosynthetic mechanism and pathway for construction of violacein core." Appl Microbiol Biotechnol 91(6);1463-75. PMID: 21779844
Sanchez06a: Sanchez C, Brana AF, Mendez C, Salas JA (2006). "Reevaluation of the violacein biosynthetic pathway and its relationship to indolocarbazole biosynthesis." Chembiochem 7(8);1231-40. PMID: 16874749
Shinoda07: Shinoda K, Hasegawa T, Sato H, Shinozaki M, Kuramoto H, Takamiya Y, Sato T, Nikaidou N, Watanabe T, Hoshino T (2007). "Biosynthesis of violacein: a genuine intermediate, protoviolaceinic acid, produced by VioABDE, and insight into VioC function." Chem Commun (Camb) (40);4140-2. PMID: 17925955
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