Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: arogenate dehydratase

Species: Nicotiana sylvestris

Gene-Reaction Schematic: ?


Enzymatic reaction of: arogenate dehydratase

EC Number: 4.2.1.91

L-arogenate + H+ <=> CO2 + L-phenylalanine + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: superpathway of phenylalanine and tyrosine biosynthesis , phenylalanine biosynthesis II

Summary:
The enzyme activity of N. silvestris arogenate dehydratase is inhibited by phenylalanine and activated by tyrosine. The maximum activation was observed at 0.25 mM of tyrosine. Zn2+ or Cu2+ at 2.5 mM completely abolished the enzyme activity, and Co2+ reduced the activity by 50%, whereas Mg2+ and Mn2+ had no effects at the same concentration.

The optimum temperature is 32 oC. The optimum pH is between 8.5 and 9.5.

Activators (Allosteric): L-tyrosine [Jung86]

Inhibitors (Unknown Mechanism): L-phenylalanine [Jung86] , Zn2+ [Jung86] , Cu2+ [Jung86] , Co2+ [Jung86]

Primary Physiological Regulators of Enzyme Activity: L-tyrosine , L-phenylalanine

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-arogenate
300.0
[Jung86]


References

Jung86: Jung E, Zamir LO, Jensen RA (1986). "Chloroplasts of higher plants synthesize L-phenylalanine via L-arogenate." Proc Natl Acad Sci U S A 83(19);7231-5. PMID: 3463961


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc14.