|Gene:||Dnmt1||Accession Number: G-6561 (MetaCyc)|
Species: Rattus norvegicus
The approximate molecular weight was determined by sedimentation analysis in a sucrose gradient [Simon78]. The predicted amino acid sequence showed 88.3% homology with the mouse sequence and 64.2% with the human sequence. A putative catalytic domain showed 93.8% homology with the mouse sequence and 89.5% with the human sequence [Kimura98].
|Map Position: [19,927,666 <- 19,973,220]|
Molecular Weight of Polypeptide: 186.0 kD (from nucleotide sequence), 115 kD (experimental) [Simon78 ]
Relationship Links: InterPro:IN-FAMILY:IPR001025 , InterPro:IN-FAMILY:IPR001525 , InterPro:IN-FAMILY:IPR002857 , InterPro:IN-FAMILY:IPR010506 , InterPro:IN-FAMILY:IPR017198 , InterPro:IN-FAMILY:IPR018117 , InterPro:IN-FAMILY:IPR022702 , Panther:IN-FAMILY:PTHR10629 , Pfam:IN-FAMILY:PF00145 , Pfam:IN-FAMILY:PF01426 , Pfam:IN-FAMILY:PF02008 , Pfam:IN-FAMILY:PF06464 , Pfam:IN-FAMILY:PF12047 , Prints:IN-FAMILY:PR00105 , Prosite:IN-FAMILY:PS00094 , Prosite:IN-FAMILY:PS00095 , Prosite:IN-FAMILY:PS51038 , Prosite:IN-FAMILY:PS51058 , Prosite:IN-FAMILY:PS51679 , Smart:IN-FAMILY:SM00439
Enzymatic reaction of: DNA (cytosine-5) methyltransferase
EC Number: 22.214.171.124
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is physiologically favored in the direction shown.
The enzyme partially purified from rat liver specifically produced 5-methylcytosine. The other product, S-adenosyl -homocysteine, competitively inhibited the reaction with a Ki of 0.33 μM [Simon78].
Kimura98: Kimura H, Takeda T, Tanaka S, Ogawa T, Shiota K (1998). "Expression of rat DNA (cytosine-5) methyltransferase (DNA MTase) in rodent trophoblast giant cells: molecular cloning and characterization of rat DNA MTase." Biochem Biophys Res Commun 253(2);495-501. PMID: 9878564
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