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MetaCyc Enzyme: DNA (cytosine-5) methyltransferase

Gene: Dnmt1 Accession Number: G-6561 (MetaCyc)

Synonyms: DNA-methylase

Species: Rattus norvegicus

Summary:
The approximate molecular weight was determined by sedimentation analysis in a sucrose gradient [Simon78]. The predicted amino acid sequence showed 88.3% homology with the mouse sequence and 64.2% with the human sequence. A putative catalytic domain showed 93.8% homology with the mouse sequence and 89.5% with the human sequence [Kimura98].

Map Position: [19,927,666 <- 19,973,220]

Molecular Weight of Polypeptide: 186.0 kD (from nucleotide sequence), 115 kD (experimental) [Simon78 ]

Unification Links: Entrez-gene:84350 , ModBase:Q9Z330 , PhosphoSite:Q9Z330 , Pride:Q9Z330 , Protein Model Portal:Q9Z330 , String:10116.ENSRNOP00000063029 , Swiss-Model:Q9Z330 , UniProt:Q9Z330

Relationship Links: InterPro:IN-FAMILY:IPR001025 , InterPro:IN-FAMILY:IPR001525 , InterPro:IN-FAMILY:IPR002857 , InterPro:IN-FAMILY:IPR010506 , InterPro:IN-FAMILY:IPR017198 , InterPro:IN-FAMILY:IPR018117 , InterPro:IN-FAMILY:IPR022702 , Panther:IN-FAMILY:PTHR10629 , Pfam:IN-FAMILY:PF00145 , Pfam:IN-FAMILY:PF01426 , Pfam:IN-FAMILY:PF02008 , Pfam:IN-FAMILY:PF06464 , Pfam:IN-FAMILY:PF12047 , Prints:IN-FAMILY:PR00105 , Prosite:IN-FAMILY:PS00094 , Prosite:IN-FAMILY:PS00095 , Prosite:IN-FAMILY:PS51038 , Prosite:IN-FAMILY:PS51058 , Prosite:IN-FAMILY:PS51679 , Smart:IN-FAMILY:SM00439

Gene-Reaction Schematic: ?


Enzymatic reaction of: DNA (cytosine-5) methyltransferase

EC Number: 2.1.1.37

a cytosine in DNA + S-adenosyl-L-methionine <=> a DNA 5-methylcytosine + S-adenosyl-L-homocysteine + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

Summary:
The enzyme partially purified from rat liver specifically produced 5-methylcytosine. The other product, S-adenosyl -homocysteine, competitively inhibited the reaction with a Ki of 0.33 μM [Simon78].

Inhibitors (Competitive): S-adenosyl-L-homocysteine [Simon78]

Kinetic Parameters:

Substrate
Km (μM)
Citations
S-adenosyl-L-methionine
2.5
[Simon78]


References

Kimura98: Kimura H, Takeda T, Tanaka S, Ogawa T, Shiota K (1998). "Expression of rat DNA (cytosine-5) methyltransferase (DNA MTase) in rodent trophoblast giant cells: molecular cloning and characterization of rat DNA MTase." Biochem Biophys Res Commun 253(2);495-501. PMID: 9878564

Simon78: Simon D, Grunert F, von Acken U, Doring HP, Kroger H (1978). "DNA-methylase from regenerating rat liver: purification and characterisation." Nucleic Acids Res 5(6);2153-67. PMID: 673848


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Jan 30, 2015, BIOCYC13A.