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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Enzyme: phosphotransacetylase

Gene: pta Accession Number: G-357 (MetaCyc)

Species: Methanosarcina thermophila

Locations: plasma membrane

Molecular Weight of Polypeptide: 35.088 kD (from nucleotide sequence) [Bairoch93a ]

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0016020 - membrane


Enzymatic reaction of: phosphotransacetylase

Synonyms: phosphate acetyltransferase, phosphoacylase, acetyl-CoA:orthophosphate acetyltransferase

EC Number: 2.3.1.8

acetyl-CoA + phosphate <=> acetyl phosphate + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

In Pathways: superpathway of methanogenesis , acetate formation from acetyl-CoA I , methanogenesis from acetate

Summary:
Phosphotransacetylase catalyzes the second acetate activation reaction in methanogenesis from acetate. The enzyme from Methanosarcina thermophila is stable to 70oC. [Lundie89, SinghWissmann95, Latimer93, Ferry92, Thauer98, Ferry93]

Cofactors or Prosthetic Groups [Lundie89]: K+

Alternative Cofactors for K+: ammonium

Inhibitors (Unknown Mechanism): Na+ [Lundie89] , phosphate [Lundie89] , sulfate [Lundie89] , arsenate [Lundie89]


References

Bairoch93a: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

Ferry92: Ferry JG (1992). "Methane from acetate." J Bacteriol 1992;174(17);5489-95. PMID: 1512186

Ferry93: Ferry, J. G. editor (1993). "Methanogenesis: Ecology, Physiology, Biochemistry & Genetics. Chapman & Hall, New York."

Latimer93: Latimer MT, Ferry JG (1993). "Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila." J Bacteriol 1993;175(21);6822-9. PMID: 8226623

Lundie89: Lundie LL, Ferry JG (1989). "Activation of acetate by Methanosarcina thermophila. Purification and characterization of phosphotransacetylase." J Biol Chem 1989;264(31);18392-6. PMID: 2808380

SinghWissmann95: Singh-Wissmann K, Ferry JG (1995). "Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila." J Bacteriol 1995;177(7);1699-702. PMID: 7896690

Thauer98: Thauer RK (1998). "Biochemistry of methanogenesis: a tribute to Marjory Stephenson." Microbiology 1998;144 ( Pt 9);2377-406. PMID: 9782487


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc13.