|Gene:||pta||Accession Number: G-357 (MetaCyc)|
Species: Methanosarcina thermophila
Locations: plasma membrane
Molecular Weight of Polypeptide: 35.088 kD (from nucleotide sequence) [Bairoch93a ]
|Cellular Component:||GO:0016020 - membrane|
Enzymatic reaction of: phosphotransacetylase
Synonyms: phosphate acetyltransferase, phosphoacylase, acetyl-CoA:orthophosphate acetyltransferase
EC Number: 18.104.22.168
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.
This reaction is reversible.
Phosphotransacetylase catalyzes the second acetate activation reaction in methanogenesis from acetate. The enzyme from Methanosarcina thermophila is stable to 70oC. [Lundie89, SinghWissmann95, Latimer93, Ferry92, Thauer98, Ferry93]
Alternative Cofactors for K+: ammonium
Latimer93: Latimer MT, Ferry JG (1993). "Cloning, sequence analysis, and hyperexpression of the genes encoding phosphotransacetylase and acetate kinase from Methanosarcina thermophila." J Bacteriol 1993;175(21);6822-9. PMID: 8226623
Lundie89: Lundie LL, Ferry JG (1989). "Activation of acetate by Methanosarcina thermophila. Purification and characterization of phosphotransacetylase." J Biol Chem 1989;264(31);18392-6. PMID: 2808380
SinghWissmann95: Singh-Wissmann K, Ferry JG (1995). "Transcriptional regulation of the phosphotransacetylase-encoding and acetate kinase-encoding genes (pta and ack) from Methanosarcina thermophila." J Bacteriol 1995;177(7);1699-702. PMID: 7896690
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