If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Biosynthesis → Amino Acids Biosynthesis → Proteinogenic Amino Acids Biosynthesis → L-arginine Biosynthesis|
Arginine biosynthesis is notable for its complexity and variability at the genetic level, and by its connection with several other pathways, such as pyrimidine and polyamine biosynthesis, and certain degradative pathways. The initial steps of the arginine biosynthetic pathways proceed via N-acetylated intermediates. The presumed reason for this is that the acetylation prevents the spontaneous cyclization of glutamate derivatives, which leads to proline biosynthesis, thus keeping the two pathways separate [Caldovic03]. A variation of the pathway found in some archaebacteria and hyperthermophilic bacteria utilizes a dedicated carrier protein to protect the intermediates instead of acetylation.
Several alternative arginine biosynthetic pathways have evolved in bacteria:
In L-arginine biosynthesis I (via L-ornithine), which is not very common, the key intermediate N-acetyl-L-glutamate (NAG) is formed by the enzyme EC 18.104.22.168, amino-acid N-acetyltransferase. A second key intermediate downstream, N-acetyl-L-ornithine, is hydrolyzed by the enzyme EC 22.214.171.124, acetylornithine deacetylase, releasing acetate, and forming the arginine precursor L-ornithine.
In L-arginine biosynthesis II (acetyl cycle)), which is found in most of the prokaryotic and eukaryotic microbes investigated up to now, the two reactions mentioned above are linked: the acetyl group which is removed from N-acetyl-L-ornithine is recycled onto glutamate, regenerating N-acetyl-L-glutamate (NAG). This recycling is performed by the enzyme EC 126.96.36.199, glutamate N-acetyltransferase. This pathway is considered more evolved, since the overall reaction is energetically more favorable [Cunin86].
The variant L-arginine biosynthesis III (via N-acetyl-L-citrulline) is found in several eubacteria. While in the two pathways mentioned above N-acetyl-L-ornithine is deacetylate to L-ornithine, which is subsequntly transcarbamoylated to form L-citrulline, in this variant N-acetyl-L-ornithine is not deacetylated. Instead, it is transcarbamoylated directly by the enzyme EC 188.8.131.52, N-acetylornithine carbamoyltransferase, resulting in N-acetyl-L-citrulline. The enzyme acetylornithine deacetylase can accept N-acetyl-L-citrulline as a substrate, and deacetylates it into L-citrulline [Morizono06].
The variant L-arginine biosynthesis IV (archaebacteria), which has been described in the archaebacterium Sulfolobus acidocaldarius, involves early intermediates that are protected not by acetylation but by a carrier protein (the LysW [LysW L-2-aminoadipate carrier protein]), which participates in the biosynthesis of both L-arginine and L-leucine in these organsims [Ouchi13].
About This Pathway
This linear variant of the arginine biosynthesis pathway has been reported from the Enterobacteriaceae [Cunin86], the Vibrionaceae [Xu00], and the Gram-negative bacterium Myxococcus xanthus [Harris98]. The pathway was also described in the archaeon Sulfolobus solfataricus [VandeCasteele90], but in most likelihood that organism possesses a different variant of the pathway (see L-arginine biosynthesis IV (archaebacteria)).
In this pathway glutamate is acetylated to N-acetyl-glutamate (NAG) by the enzyme NAG synthase, encoded by the argA gene. The acetyl donor for this reaction is acetyl-CoA. NAG is then converted in several enzymatic steps to N-acetyl-ornithine. The acetyl group is removed in the form of acetate by the enzyme acetylornithine deacetylase, encoded by the argE gene. Ornithine is then combined with carbamoyl phosphate to form citrulline, which is converted in two steps to L-arginine.
Superpathways: superpathway of arginine and polyamine biosynthesis
Subpathways: L-ornithine biosynthesis
Unification Links: EcoCyc:ARGSYN-PWY
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