If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Biosynthesis → Carbohydrates Biosynthesis|
Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655
Expected Taxonomic Range: Proteobacteria
Colanic acid, also known as the M antigen, is an extracellular polysaccharide found in Enterobacteriaceae. It is a polyanionic heteropolysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate sugars that are nonstoichiometrically decorated with O-acetyl and pyruvate side chains [Grant69, Garegg71]. The sugars need to be activated in the form of nucleotide sugars (UDP-α-D-glucose, GDP-β-L-fucose, UDP-α-D-galactose and UDP-α-D-glucuronate, respectively) prior to their assembly [Stevenson96, Stout96].
The colanic acid polysaccharide repeat is assembled on the membrane lipid all-trans-dodecaprenyl diphosphate by a series of glycosyl transferases on the cytoplasmic face of the inner membrane, after which the single repeat is flipped to the periplasmic side and polymerized by the Wzy-dependent pathway (reviewed in [Raetz02] and [Whitfield06]). Subsequently, the polymer is believed to be cleaved from the all-trans-dodecaprenyl diphosphate anchor, transported across the periplasm, and excreted into the extracellular space, although this process is not well understood.
Colanic acid biosynthesis has been linked to a cluster of 19 genes, which were named wca [Stevenson96, Stout96]. This gene cluster is tightly regulated by a complex signal transduction cascade governed by the rcs (regulator of capsule synthesis) phosphorelay system [Gottesman85, Majdalani05a].
Subpathways: GDP-L-fucose biosynthesis I (from GDP-D-mannose) , GDP-mannose biosynthesis , UDP-glucose biosynthesis , UDP-D-galactose biosynthesis , UDP-α-D-glucuronate biosynthesis (from UDP-glucose)
Unification Links: EcoCyc:COLANSYN-PWY
Created 02-Dec-1997 by Pellegrini-Toole A , Marine Biological Laboratory
Revised 29-May-2008 by Caspi R , SRI International
Revised 13-Jun-2008 by Keseler I , SRI International
Revised 15-Aug-2013 by Caspi R , SRI International
Garegg71: Garegg PJ, Lindberg B, Onn T, Sutherland IW (1971). "Comparative structural studies on the M-antigen from Salmonella typhimurium. Escherichia coli and Aerobacter cloacae." Acta Chem Scand 25(6);2103-8. PMID: 4941627
Gottesman85: Gottesman S, Trisler P, Torres-Cabassa A (1985). "Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes." J Bacteriol 162(3);1111-9. PMID: 3888955
Stevenson96: Stevenson G, Andrianopoulos K, Hobbs M, Reeves PR (1996). "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." J Bacteriol 1996;178(16);4885-93. PMID: 8759852
Accorsi89: Accorsi A, Piatti E, Piacentini MP, Gini S, Fazi A (1989). "Isoenzymes of phosphoglucomutase from human red blood cells: isolation and kinetic properties." Prep Biochem 19(3);251-71. PMID: 2533352
Ahn11: Ahn J, Chung BK, Lee DY, Park M, Karimi IA, Jung JK, Lee H (2011). "NADPH-dependent pgi-gene knockout Escherichia coli metabolism producing shikimate on different carbon sources." FEMS Microbiol Lett 324(1);10-6. PMID: 22092758
Al12: Al Mamun AA, Lombardo MJ, Shee C, Lisewski AM, Gonzalez C, Lin D, Nehring RB, Saint-Ruf C, Gibson JL, Frisch RL, Lichtarge O, Hastings PJ, Rosenberg SM (2012). "Identity and function of a large gene network underlying mutagenic repair of DNA breaks." Science 338(6112);1344-8. PMID: 23224554
Albermann01: Albermann C, Piepersberg W (2001). "Expression and identification of the RfbE protein from Vibrio cholerae O1 and its use for the enzymatic synthesis of GDP-D-perosamine." Glycobiology 11(8);655-61. PMID: 11479276
Albermann01a: Albermann C, Piepersberg W, Wehmeier UF (2001). "Synthesis of the milk oligosaccharide 2'-fucosyllactose using recombinant bacterial enzymes." Carbohydr Res 334(2);97-103. PMID: 11502265
Amor97: Amor PA, Whitfield C (1997). "Molecular and functional analysis of genes required for expression of group IB K antigens in Escherichia coli: characterization of the his-region containing gene clusters for multiple cell-surface polysaccharides." Mol Microbiol 26(1);145-61. PMID: 9383197
Andrianopoulos98: Andrianopoulos K, Wang L, Reeves PR (1998). "Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12." J Bacteriol 1998;180(4);998-1001. PMID: 9473059
Aoyama94a: Aoyama K, Haase AM, Reeves PR (1994). "Evidence for effect of random genetic drift on G+C content after lateral transfer of fucose pathway genes to Escherichia coli K-12." Mol Biol Evol 1994;11(6);829-38. PMID: 7815923
Arabshahi88: Arabshahi A, Flentke GR, Frey PA (1988). "Uridine diphosphate galactose 4-epimerase. pH dependence of the reduction of NAD+ by a substrate analog." J Biol Chem 263(6);2638-43. PMID: 3277958
Arrecubieta94: Arrecubieta C, Lopez R, Garcia E (1994). "Molecular characterization of cap3A, a gene from the operon required for the synthesis of the capsule of Streptococcus pneumoniae type 3: sequencing of mutations responsible for the unencapsulated phenotype and localization of the capsular cluster on the pneumococcal chromosome." J Bacteriol 176(20);6375-83. PMID: 7929009
Barber06: Barber C, Rosti J, Rawat A, Findlay K, Roberts K, Seifert GJ (2006). "Distinct properties of the five UDP-D-glucose/UDP-D-galactose 4-epimerase isoforms of arabidopsis thaliana." J Biol Chem. PMID: 16644739
Bauer91: Bauer AJ, Rayment I, Frey PA, Holden HM (1991). "The isolation, purification, and preliminary crystallographic characterization of UDP-galactose-4-epimerase from Escherichia coli." Proteins 9(2);135-42. PMID: 2008433
Bauer92: Bauer AJ, Rayment I, Frey PA, Holden HM (1992). "The molecular structure of UDP-galactose 4-epimerase from Escherichia coli determined at 2.5 A resolution." Proteins 12(4);372-81. PMID: 1579570
Baveja86: Baveja UK, Jyoti AS, Kaur M, Agarwal DS, Anand BS, Nanda R (1986). "Isoenzyme studies of Giardia lamblia isolated from symptomatic cases." Aust J Exp Biol Med Sci 64 ( Pt 2);119-26. PMID: 2943257
Berger01: Berger E, Arabshahi A, Wei Y, Schilling JF, Frey PA (2001). "Acid-base catalysis by UDP-galactose 4-epimerase: correlations of kinetically measured acid dissociation constants with thermodynamic values for tyrosine 149." Biochemistry 40(22);6699-705. PMID: 11380265
Bhattacharyya99: Bhattacharyya U, Dhar G, Bhaduri A (1999). "An arginine residue is essential for stretching and binding of the substrate on UDP-glucose-4-epimerase from Escherichia coli. Use of a stacked and quenched uridine nucleotide fluorophore as probe." J Biol Chem 274(21);14573-8. PMID: 10329648
Bindschedler05: Bindschedler LV, Wheatley E, Gay E, Cole J, Cottage A, Bolwell GP (2005). "Characterisation and expression of the pathway from UDP-glucose to UDP-xylose in differentiating tobacco tissue." Plant Mol Biol 57(2);285-301. PMID: 15821883
Showing only 20 references. To show more, press the button "Show all references".
©2015 SRI International, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493