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MetaCyc Pathway: N-acetylglucosamine degradation I

Note: a dashed line (without arrowheads) between two compound names is meant to imply that the two names are just different instantiations of the same compound -- i.e. one may be a specific name and the other a general name, or they may both represent the same compound in different stages of a polymerization-type pathway. This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Synonyms: glucosamine degradation, N-acetyl-glucosamine degradation

Superclasses: Degradation/Utilization/Assimilation Amines and Polyamines Degradation N-acetylglucosamine degradation
Degradation/Utilization/Assimilation Secondary Metabolites Degradation Sugar Derivatives Degradation N-acetylglucosamine degradation

Some taxa known to possess this pathway include ? : Escherichia coli K-12 substr. MG1655

Expected Taxonomic Range: Bacteria , Fungi , Metazoa

Summary:
N-acetylglucosamine, which is relatively abundant in nature, being a constituent of chitosan, chitin, the cell walls of fungi as well as the cell walls, capsular polysaccharides, and outer membranes of bacteria, is an excellent total source of carbon, energy and nitrogen for Escherichia coli. Because it enters the cell via a specific phosphotransferase system (PTS), the first intracellular species is N-acetyl-D-glucosamine-6-phosphate. Following the sequential removal of its acetyl and amino groups, it enters glycolysis as D-fructose-6-phosphate and hence flows through the pathways of central metabolism to satisfy the cell's need for precursor metabolites, reducing power, and metabolic energy.

Growth on glucosamine is slower than growth on N-acetylglucosamine. The limiting factor appears to be the level of expression of glucosamine-6-phosphate deaminase [AlvarezAnorve05].

Review: Mayer, C. and W. Boos, Hexose/Pentose and Hexitol/Pentitol Metabolism. EcoSal Module 3.4.1 [ECOSAL]

Superpathways: superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation , superpathway of N-acetylneuraminate degradation , N-acetylglucosamine degradation II

Unification Links: EcoCyc:GLUAMCAT-PWY

Credits:
Created 18-Oct-1995 by Riley M , Marine Biological Laboratory
Revised 09-May-2006 by Ingraham JL , UC Davis
Revised 27-Jun-2007 by Keseler I , SRI International


References

AlvarezAnorve05: Alvarez-Anorve LI, Calcagno ML, Plumbridge J (2005). "Why does Escherichia coli grow more slowly on glucosamine than on N-acetylglucosamine? Effects of enzyme levels and allosteric activation of GlcN6P deaminase (NagB) on growth rates." J Bacteriol 187(9);2974-82. PMID: 15838023

ECOSAL: EcoSal "Escherichia coli and Salmonella: Cellular and Molecular Biology." Online edition.

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Altamirano87: Altamirano MM, Mulliert G, Calcagno M (1987). "Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coli." Arch Biochem Biophys 1987;258(1);95-100. PMID: 2821923

Altamirano90: Altamirano MM, Calcagno M (1990). "Zinc binding and its trapping by allosteric transition in glucosamine-6-phosphate deaminase from Escherichia coli." Biochim Biophys Acta 1038(3);291-4. PMID: 2111170

Altamirano92: Altamirano MM, Plumbridge JA, Calcagno ML (1992). "Identification of two cysteine residues forming a pair of vicinal thiols in glucosamine-6-phosphate deaminase from Escherichia coli and a study of their functional role by site-directed mutagenesis." Biochemistry 31(4);1153-8. PMID: 1734962

Altamirano93: Altamirano MM, Plumbridge JA, Barba HA, Calcagno ML (1993). "Glucosamine-6-phosphate deaminase from Escherichia coli has a trimer of dimers structure with three intersubunit disulphides." Biochem J 295 ( Pt 3);645-8. PMID: 8240271

Altamirano94: Altamirano MM, Hernandez-Arana A, Tello-Solis S, Calcagno ML (1994). "Spectrochemical evidence for the presence of a tyrosine residue in the allosteric site of glucosamine-6-phosphate deaminase from Escherichia coli." Eur J Biochem 1994;220(2);409-13. PMID: 8125098

Altamirano95: Altamirano MM, Plumbridge JA, Horjales E, Calcagno ML (1995). "Asymmetric allosteric activation of Escherichia coli glucosamine-6-phosphate deaminase produced by replacements of Tyr 121." Biochemistry 34(18);6074-82. PMID: 7742311

AlvarezAnorve09: Alvarez-Anorve LI, Bustos-Jaimes I, Calcagno ML, Plumbridge J (2009). "Allosteric regulation of glucosamine-6-phosphate deaminase (NagB) and growth of Escherichia coli on glucosamine." J Bacteriol 191(20);6401-7. PMID: 19700525

Bassler91: Bassler BL, Yu C, Lee YC, Roseman S (1991). "Chitin utilization by marine bacteria. Degradation and catabolism of chitin oligosaccharides by Vibrio furnissii." J Biol Chem 266(36);24276-86. PMID: 1761533

BRENDA14: BRENDA team (2014). "Imported from BRENDA version existing on Aug 2014." http://www.brenda-enzymes.org.

BustosJaimes01: Bustos-Jaimes I, Calcagno ML (2001). "Allosteric transition and substrate binding are entropy-driven in glucosamine-6-phosphate deaminase from Escherichia coli." Arch Biochem Biophys 394(2);156-60. PMID: 11594728

BustosJaimes02: Bustos-Jaimes I, Sosa-Peinado A, Rudino-Pinera E, Horjales E, Calcagno ML (2002). "On the role of the conformational flexibility of the active-site lid on the allosteric kinetics of glucosamine-6-phosphate deaminase." J Mol Biol 319(1);183-9. PMID: 12051945

BustosJaimes05: Bustos-Jaimes I, Ramirez-Costa M, De Anda-Aguilar L, Hinojosa-Ocana P, Calcagno ML (2005). "Evidence for two different mechanisms triggering the change in quaternary structure of the allosteric enzyme, glucosamine-6-phosphate deaminase." Biochemistry 44(4);1127-35. PMID: 15667206

Byers96: Byers HL, Homer KA, Beighton D (1996). "Utilization of sialic acid by viridans streptococci." J Dent Res 1996;75(8);1564-71. PMID: 8906124

Calcagno84: Calcagno M, Campos PJ, Mulliert G, Suastegui J (1984). "Purification, molecular and kinetic properties of glucosamine-6-phosphate isomerase (deaminase) from Escherichia coli." Biochim Biophys Acta 1984;787(2);165-73. PMID: 6375729

Cisneros04: Cisneros DA, Montero-Moran GM, Lara-Gonzalez S, Calcagno ML (2004). "Inversion of the allosteric response of Escherichia coli glucosamine-6-P deaminase to N-acetylglucosamine 6-P, by single amino acid replacements." Arch Biochem Biophys 421(1);77-84. PMID: 14678787

Comb58: Comb DG, Roseman S (1958). "Glucosamine metabolism. IV. Glucosamine-6-phosphate deaminase." J Biol Chem 232(2);807-27. PMID: 13549465

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Ferreira00: Ferreira FM, Mendoza-Hernandez G, Calcagno ML, Minauro F, Delboni LF, Oliva G (2000). "Crystallization and preliminary crystallographic analysis of N-acetylglucosamine 6-phosphate deacetylase from Escherichia coli." Acta Crystallogr D Biol Crystallogr 56(Pt 5);670-2. PMID: 10771446

Ferreira06: Ferreira FM, Mendoza-Hernandez G, Castaneda-Bueno M, Aparicio R, Fischer H, Calcagno ML, Oliva G (2006). "Structural analysis of N-acetylglucosamine-6-phosphate deacetylase apoenzyme from Escherichia coli." J Mol Biol 359(2);308-21. PMID: 16630633

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

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Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc12.