This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Biosynthesis → Fatty Acid and Lipid Biosynthesis|
Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655
Expected Taxonomic Range: Proteobacteria
Bacterial lipopolysaccharides (LPS) typically consist of a hydrophobic domain inserted into the outer membrane known as lipid A (or endotoxin), a phosphorylated "core" oligosaccharide and a distal polysaccharide (or O antigen). The core oligosaccharides are conceptually divided into two regions: inner core and outer core. The inner core is highly conserved, comprises three deoxy-D-manno-octulosonic acid (KDO) and L-glycero-D-manno-heptose (Hep) and is often phosphorylated. The inner core oligosaccharide plays a critical role in essential barrier function of the outer membrane. The outer core comprises a tri-hexose backbone modified with varying side-branch substitutions of hexose and acetamidohexose residues. The outer core provides an attachment site for O-antigen. Strains of Escherichia coli K12 normally do not make O-antigen, unless a mutation in the O-antigen operon is corrected [Stevenson94].
About This Pathway
The completed lipid A-KDO2 serves as the acceptor on which the core oligosaccharide chains are assembled by sequential glycosyl transfer from nucleotide sugar precursors. This process involves a co-ordinated complex of membrane-associated glycosyltransferases acting at the cytoplasmic face of the plasma membrane. Many of the enzymes are predicted to be peripheral proteins.
Escherichia coli mutants that lack the heptose region of the inner core display "deep rough" phenotypes and causes instability of the outer membrane. These mutants lose expression of pili and flagella and secrete hemolysin with reduced activity. Mutations that eliminate synthesis of the outer core result in enhanced susceptibility to some hydrophobic compouns due to an indirect effect on core phosphorylation.
Superpathways: superpathway of lipopolysaccharide biosynthesis
Unification Links: EcoCyc:LIPA-CORESYN-PWY
Heinrichs98: Heinrichs DE, Yethon JA, Whitfield C (1998). "Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica." Mol Microbiol 30(2);221-32. PMID: 9791168
Stevenson94: Stevenson G, Neal B, Liu D, Hobbs M, Packer NH, Batley M, Redmond JW, Lindquist L, Reeves P (1994). "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." J Bacteriol 1994;176(13);4144-56. PMID: 7517391
Agladze05: Agladze K, Wang X, Romeo T (2005). "Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA." J Bacteriol 187(24);8237-46. PMID: 16321928
Austin90: Austin EA, Graves JF, Hite LA, Parker CT, Schnaitman CA (1990). "Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus." J Bacteriol 172(9);5312-25. PMID: 2168379
Bociek15: Bociek K, Ferluga S, Mardirossian M, Benincasa M, Tossi A, Gennaro R, Scocchi M (2015). "Lipopolysaccharide Phosphorylation by the WaaY Kinase Affects the Susceptibility of Escherichia coli to the Human Antimicrobial Peptide LL-37." J Biol Chem 290(32);19933-41. PMID: 26100635
Brabetz97: Brabetz W, Muller-Loennies S, Holst O, Brade H (1997). "Deletion of the heptosyltransferase genes rfaC and rfaF in Escherichia coli K-12 results in an Re-type lipopolysaccharide with a high degree of 2-aminoethanol phosphate substitution." Eur J Biochem 247(2);716-24. PMID: 9266718
CarlsonBanning13: Carlson-Banning KM, Chou A, Liu Z, Hamill RJ, Song Y, Zechiedrich L (2013). "Toward repurposing ciclopirox as an antibiotic against drug-resistant Acinetobacter baumannii, Escherichia coli, and Klebsiella pneumoniae." PLoS One 8(7);e69646. PMID: 23936064
Chen93c: Chen L, Coleman WG (1993). "Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis." J Bacteriol 175(9);2534-40. PMID: 8478319
Corsaro09: Corsaro MM, Parrilli E, Lanzetta R, Naldi T, Pieretti G, Lindner B, Carpentieri A, Parrilli M, Tutino ML (2009). "The presence of OMP inclusion bodies in a Escherichia coli K-12 mutated strain is not related to lipopolysaccharide structure." J Biochem 146(2);231-40. PMID: 19364804
Creeger79: Creeger ES, Rothfield LI (1979). "Cloning of genes for bacterial glycosyltransferases. I. Selection of hybrid plasmids carrying genes for two glucosyltransferases." J Biol Chem 254(3);804-10. PMID: 368061
Czyzyk11: Czyzyk DJ, Liu C, Taylor EA (2011). "Lipopolysaccharide biosynthesis without the lipids: recognition promiscuity of Escherichia coli heptosyltransferase I." Biochemistry 50(49);10570-2. PMID: 22059588
De05: De Spiegeleer P, Sermon J, Vanoirbeek K, Aertsen A, Michiels CW (2005). "Role of porins in sensitivity of Escherichia coli to antibacterial activity of the lactoperoxidase enzyme system." Appl Environ Microbiol 71(7);3512-8. PMID: 16000755
De05a: De Spiegeleer P, Vanoirbeek K, Lietaert A, Sermon J, Aertsen A, Michiels CW (2005). "Investigation into the resistance of lactoperoxidase tolerant Escherichia coli mutants to different forms of oxidative stress." FEMS Microbiol Lett 252(2);315-9. PMID: 16209909
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Durka12: Durka M, Buffet K, Iehl J, Holler M, Nierengarten JF, Vincent SP (2012). "The inhibition of liposaccharide heptosyltransferase WaaC with multivalent glycosylated fullerenes: a new mode of glycosyltransferase inhibition." Chemistry 18(2);641-51. PMID: 22147564
Endo69: Endo A, Rothfield L (1969). "Studies of a phospholipid-requiring bacterial enzyme. I. Purification and properties of uridine diphosphate galactose: lipopolysaccharide alpha-3-galactosyl transferase." Biochemistry 8(9);3500-7. PMID: 4898284
GarciaVerdugo05: Garcia-Verdugo I, Sanchez-Barbero F, Soldau K, Tobias PS, Casals C (2005). "Interaction of SP-A (surfactant protein A) with bacterial rough lipopolysaccharide (Re-LPS), and effects of SP-A on the binding of Re-LPS to CD14 and LPS-binding protein." Biochem J 391(Pt 1);115-24. PMID: 15932345
Genevaux99: Genevaux P, Bauda P, DuBow MS, Oudega B (1999). "Identification of Tn10 insertions in the rfaG, rfaP, and galU genes involved in lipopolysaccharide core biosynthesis that affect Escherichia coli adhesion." Arch Microbiol 172(1);1-8. PMID: 10398745
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