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discounted EARLY registration ends Dec 31, 2014
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discounted EARLY registration ends Dec 31, 2014
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for maintenance.
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discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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for maintenance.
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discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
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MetaCyc Pathway: glutathione-mediated detoxification I

Enzyme View:

This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Superclasses: Degradation/Utilization/Assimilation Degradation/Utilization/Assimilation - Other
Detoxification

Some taxa known to possess this pathway include ? : Homo sapiens , Rattus norvegicus , Sus scrofa

Expected Taxonomic Range: Opisthokonta

Summary:
Please note: This is a general detoxification pathway, where the first step can be catalyzed by many different enzymes, collectively known as glutathione transferases, or GSTs. In the first reaction, R may be an aliphatic, aromatic or heterocyclic group, and X may be a sulfate, nitrile or halide group.

General Background

Thiols play several major roles in the cell; they help maintain the redox balance, keeping a reduced environment (see the pathway glutathione redox reactions II), they fight reactive oxygen and nitrogen species (ROS and NOS, respectively), and they are involved in the detoxification of many other toxins and stress-inducing factors.

In most organisms the major thiol is the tripeptide glutathione (γ-Glu-Cys-Gly, known as GSH). GSH is active against toxins by a process that involves multiple enzymes, and in the case of eukaryotes, occurs across multiple organs.

About This Pathway

In this important detoxification mechanism GSH binds to electrophilic chemicals, forming conjugates which are exported from the cell. These conjugation reactions have been demonstrated for a multitude of foreign chemicals, as well as endogenous reactive intermediates. GSH has been shown to form thioether conjugates with leukotrienes, a prostaglandin, hepoxilin, nitric oxide, hydroxyalkenals, L-ascorbate, L-dopa, dopamine, and maleate, and it forms thioesters with L-cysteine, coenzyme A, proteins, and other cellular thiols [Wang98a]. In addition, GSH also binds endogenous metals, such as copper, selenium, chromium, and zinc, via nonenzymatic reactions.

The first step is catalyzed by glutathione S-transferase, a family of enzymes found mainly in the cytosol. Once formed, the GSH-toxin conjugates are metabolized by the same degradative enzymes that metabolize GSH (see γ-glutamyl cycle). The GSH-toxin conjugate is transported out of the cell, where it is subsequently degraded by γ-glutamyl hydrolase or γ-glutamyl transpeptidase, and dipeptidases. The breakdown products of the GSH-toxin conjugates (glutamate and glycine) are reabsorbed and can be used for GSH synthesis. The an L-cysteine-S-conjugate that is left is also transported back into the cell, where it can be metabolized in different ways.

One route is the acetylation of the amino group of the cysteinyl residue by intracellular N-acetyltransferases to form the corresponding mercapturic acids (N-acetylcysteine S-conjugates). The addition of the N-acetylcysteine moiety generally increases the compound's polarity and water solubility, and converts neutral compounds to anions, facilitating their transport across cell membranes and their excretion from the organism [Boyland69]. Mercapturic acids are released into the circulation or bile [Hinchman91]; some are eventually excreted in urine, and some may undergo further metabolism.

A second route for an L-cysteine-S-conjugate is the breakage of the carbon-sulfur bond, catalyzed by cysteine-S-conjugate β-lyase resulting in the formation of thiols, pyruvate and ammonia [Larsen85, Field96, Vuilleumier97, Cooper05]. This enzymatic activity has been shown in both eukaryotes [Seta67] and prokaryotes [Yoshida02]. The thiols which are produced in this reaction are most likely oxidized to sulfonates, or methylated to methylsulfinyl or methylsulfonyl derivatives.

Yet additional fates for an L-cysteine-S-conjugate are possible. It has been shown that these compounds can be substrates for other enzymes, including aminotransferase and L-amino acid oxidase [Cooper05].

While this pathway has been studied in detail in eukaryotes, considerably less in known about bacteria. Recent sequencing projects of bacterial genomes have revealed the presence of numerous glutathione S-transferase genes in many microbes [Vuilleumier97, Vuilleumier02]. Biochemical evidence for the activity of such enzymes is also abundant. It has also been shown that glutathione conjugates can be metabolized to cysteine conjugates by the action of soil microorganisms, although the specific enzymes involved in the process have not been identified [Field96]. It seems that bacteria are more likely to continue by the cysteine conjugate lyase route, as there are several reports for this activity in bacteria [Larsen85, Yoshida02].

Variants: (Z)-butanethiol-S-oxide biosynthesis , 4-hydroxy-2-nonenal detoxification , ascorbate glutathione cycle , baicalein degradation (hydrogen peroxide detoxification) , cyanate degradation , detoxification of reactive carbonyls in chloroplasts , farnesylcysteine salvage pathway , fluoroacetate degradation , furfural degradation , glutathione-mediated detoxification II , mycothiol-mediated detoxification , oxidized GTP and dGTP detoxification , seleno-amino acid detoxification and volatilization III , superpathway of seleno-compound metabolism

Credits:
Created 12-Jul-2005 by Caspi R , SRI International


References

Boyland69: Boyland E, Chasseaud LF (1969). "The role of glutathione and glutathione S-transferases in mercapturic acid biosynthesis." Adv Enzymol Relat Areas Mol Biol 32;173-219. PMID: 4892500

Cooper05: Cooper AJ, Pinto JT (2005). "Aminotransferase, L-amino acid oxidase and beta-lyase reactions involving L-cysteine S-conjugates found in allium extracts. Relevance to biological activity?." Biochem Pharmacol 69(2);209-20. PMID: 15627473

Field96: Field, J. A., Thurman, E. M. (1996). "Glutathione conjugation and contaminant transformation." Environ. Sci. Tech. 30 (5): 1413-1418.

Hinchman91: Hinchman CA, Matsumoto H, Simmons TW, Ballatori N (1991). "Intrahepatic conversion of a glutathione conjugate to its mercapturic acid. Metabolism of 1-chloro-2,4-dinitrobenzene in isolated perfused rat and guinea pig livers." J Biol Chem 266(33);22179-85. PMID: 1939239

Hinchman94: Hinchman CA, Ballatori N (1994). "Glutathione conjugation and conversion to mercapturic acids can occur as an intrahepatic process." J Toxicol Environ Health 41(4);387-409. PMID: 8145281

Larsen85: Larsen GL (1985). "Distribution of cysteine conjugate beta-lyase in gastrointestinal bacteria and in the environment." Xenobiotica 15(3);199-209. PMID: 4024656

Seta67: Seta K, Kawamura T, Ueda Y, Katsura S, Terui Y (1967). "[Case of squamous cell carcinoma of the stomach]." Gan No Rinsho 13(10);937-40. PMID: 5627473

Vuilleumier02: Vuilleumier S, Pagni M (2002). "The elusive roles of bacterial glutathione S-transferases: new lessons from genomes." Appl Microbiol Biotechnol 58(2);138-46. PMID: 11876405

Vuilleumier97: Vuilleumier S (1997). "Bacterial glutathione S-transferases: what are they good for?." J Bacteriol 179(5);1431-41. PMID: 9045797

Wang98a: Wang W, Ballatori N (1998). "Endogenous glutathione conjugates: occurrence and biological functions." Pharmacol Rev 50(3);335-56. PMID: 9755286

Yoshida02: Yoshida Y, Nakano Y, Amano A, Yoshimura M, Fukamachi H, Oho T, Koga T (2002). "lcd from Streptococcus anginosus encodes a C-S lyase with alpha,beta-elimination activity that degrades L-cysteine." Microbiology 148(Pt 12);3961-70. PMID: 12480900

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Ahmad93: Ahmad H, Singhal SS, Saxena M, Awasthi YC (1993). "Characterization of two novel subunits of the alpha-class glutathione S-transferases of human liver." Biochim Biophys Acta 1161(2-3);333-6. PMID: 8431482

Aigner96: Aigner A, Jager M, Pasternack R, Weber P, Wienke D, Wolf S (1996). "Purification and characterization of cysteine-S-conjugate N-acetyltransferase from pig kidney." Biochem J 317 ( Pt 1);213-8. PMID: 8694766

Blackburn98: Blackburn AC, Woollatt E, Sutherland GR, Board PG (1998). "Characterization and chromosome location of the gene GSTZ1 encoding the human Zeta class glutathione transferase and maleylacetoacetate isomerase." Cytogenet Cell Genet 83(1-2);109-14. PMID: 9925947

BrazierHicks08: Brazier-Hicks M, Evans KM, Cunningham OD, Hodgson DR, Steel PG, Edwards R (2008). "Catabolism of glutathione conjugates in Arabidopsis thaliana. Role in metabolic reactivation of the herbicide safener fenclorim." J Biol Chem 283(30);21102-12. PMID: 18522943

Chandler86: Chandler CJ, Wang TT, Halsted CH (1986). "Pteroylpolyglutamate hydrolase from human jejunal brush borders. Purification and characterization." J Biol Chem 261(2);928-33. PMID: 2867095

Duffel82: Duffel MW, Jakoby WB (1982). "Cysteine S-conjugate N-acetyltransferase from rat kidney microsomes." Mol Pharmacol 21(2);444-8. PMID: 6892478

FernandezCanon99: Fernandez-Canon JM, Hejna J, Reifsteck C, Olson S, Grompe M (1999). "Gene structure, chromosomal location, and expression pattern of maleylacetoacetate isomerase." Genomics 58(3);263-9. PMID: 10373324

Green75: Green RM, Elce JS (1975). "Acetylation of S-substituted cysteines by a rat liver and kidney microsomal N-acetyltransferase." Biochem J 147(2);283-9. PMID: 241322

Grzam07: Grzam A, Martin MN, Hell R, Meyer AJ (2007). "gamma-Glutamyl transpeptidase GGT4 initiates vacuolar degradation of glutathione S-conjugates in Arabidopsis." FEBS Lett 581(17);3131-8. PMID: 17561001

Hagmann85: Hagmann W, Denzlinger C, Keppler D (1985). "Production of peptide leukotrienes in endotoxin shock." FEBS Lett 180(2);309-13. PMID: 3967766

Han04: Han Q, Li J (2004). "pH dependence, substrate specificity and inhibition of human kynurenine aminotransferase I." Eur J Biochem 271(23-24);4804-14. PMID: 15606768

Han08: Han Q, Robinson H, Li J (2008). "Crystal structure of human kynurenine aminotransferase II." J Biol Chem 283(6);3567-73. PMID: 18056995

Han09: Han Q, Robinson H, Cai T, Tagle DA, Li J (2009). "Structural insight into the inhibition of human kynurenine aminotransferase I/glutamine transaminase K." J Med Chem 52(9);2786-93. PMID: 19338303

Hiroi92: Hiroi Y, Endo Y, Natori Y (1992). "Purification and properties of an aminopeptidase from rat-liver cytosol." Arch Biochem Biophys 294(2);440-5. PMID: 1314542

Hirota85: Hirota T, Nishikawa Y, Takahagi H, Igarashi T, Kitagawa H (1985). "Simultaneous purification and properties of dehydropeptidase-I and aminopeptidase-M from rat kidney." Res Commun Chem Pathol Pharmacol 49(3);435-45. PMID: 2865778

Huangpu96: Huangpu J, Pak JH, Graham MC, Rickle SA, Graham JS (1996). "Purification and molecular analysis of an extracellular gamma-glutamyl hydrolase present in young tissues of the soybean plant." Biochem Biophys Res Commun 228(1);1-6. PMID: 8912628

Iizuka89: Iizuka M, Inoue Y, Murata K, Kimura A (1989). "Purification and some properties of glutathione S-transferase from Escherichia coli B." J Bacteriol 1989;171(11);6039-42. PMID: 2553668

Josch03: Josch C, Klotz LO, Sies H (2003). "Identification of cytosolic leucyl aminopeptidase (EC 3.4.11.1) as the major cysteinylglycine-hydrolysing activity in rat liver." Biol Chem 384(2);213-8. PMID: 12675513

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Okuno90: Okuno E, Du F, Ishikawa T, Tsujimoto M, Nakamura M, Schwarcz R, Kido R (1990). "Purification and characterization of kynurenine-pyruvate aminotransferase from rat kidney and brain." Brain Res 534(1-2);37-44. PMID: 1963565

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Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, BIOCYC13A.