This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Synonyms: 2-oxopent-4-enoate degradation, 2-ketopentenoate degradation
|Superclasses:||Degradation/Utilization/Assimilation → Carboxylates Degradation|
Some taxa known to possess this pathway include : Acidovorax sp. JS42, Bordetella sp. 10d, Burkholderia cepacia, Comamonas sp. JS765, Escherichia coli K-12 substr. MG1655, Pseudomonas putida, Pseudomonas putida F1, Pseudomonas putida mt-2, Pseudomonas sp. CF600, Ralstonia pickettii
Expected Taxonomic Range: Proteobacteria
The degradation pathway depicted here has been described in several organisms, including Escherichia coli K-12. The genes encoding the enzymes of this pathway are often found on plasmids, such as naphthalene catabolic plasmids (pWW60-22 [Platt95] and pND6-1 [Li04]), nitrobenzene catabolic plasmids (pNB1 and pNB2, [Park00]) and toluene catabolic plasmids (TOL) such as pWW102 [Aemprapa98].
In this pathway 2-oxopent-4-enoate is hydrolyzed to 4-hydroxy-2-oxopentanoate by the enzyme 2-oxopent-4-enoate hydratase. 4-hydroxy-2-oxopentanoate is broken into pyruvate and acetaldehyde, which is then converted to acetyl-CoA by acetaldehyde dehydrogenase [Harayama89, Platt95].
Superpathways: naphthalene degradation to acetyl-CoA, mandelate degradation to acetyl-CoA, 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation, superpathway of aromatic compound degradation via 3-oxoadipate, superpathway of aromatic compound degradation via 2-oxopent-4-enoate, L-tryptophan degradation XII (Geobacillus), catechol degradation I (meta-cleavage pathway), catechol degradation II (meta-cleavage pathway), L-tryptophan degradation IX, p-cumate degradation, p-cymene degradation, toluene degradation II (aerobic) (via 4-methylcatechol), toluene degradation I (aerobic) (via o-cresol), toluene degradation V (aerobic) (via toluene-cis-diol), toluene degradation IV (aerobic) (via catechol), meta cleavage pathway of aromatic compounds, superpathway of aerobic toluene degradation
Unification Links: EcoCyc:PWY-5162
Harayama89: Harayama S, Rekik M, Ngai KL, Ornston LN (1989). "Physically associated enzymes produce and metabolize 2-hydroxy-2,4-dienoate, a chemically unstable intermediate formed in catechol metabolism via meta cleavage in Pseudomonas putida." J Bacteriol 171(11);6251-8. PMID: 2681159
Li04: Li W, Shi J, Wang X, Han Y, Tong W, Ma L, Liu B, Cai B (2004). "Complete nucleotide sequence and organization of the naphthalene catabolic plasmid pND6-1 from Pseudomonas sp. strain ND6." Gene 336(2);231-40. PMID: 15246534
Platt95: Platt A, Shingler V, Taylor SC, Williams PA (1995). "The 4-hydroxy-2-oxovalerate aldolase and acetaldehyde dehydrogenase (acylating) encoded by the nahM and nahO genes of the naphthalene catabolic plasmid pWW60-22 provide further evidence of conservation of meta-cleavage pathway gene sequences." Microbiology 141 ( Pt 9);2223-33. PMID: 7496535
Dellomonaco11: Dellomonaco C, Clomburg JM, Miller EN, Gonzalez R (2011). "Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals." Nature 476(7360);355-9. PMID: 21832992
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Ferrandez97a: Ferrandez A, Garcia JL, Diaz E (1997). "Genetic characterization and expression in heterologous hosts of the 3-(3-hydroxyphenyl)propionate catabolic pathway of Escherichia coli K-12." J Bacteriol 1997;179(8);2573-81. PMID: 9098055
Fischer13: Fischer B, Boutserin S, Mazon H, Collin S, Branlant G, Gruez A, Talfournier F (2013). "Catalytic properties of a bacterial acylating acetaldehyde dehydrogenase: evidence for several active oligomeric states and coenzyme A activation upon binding." Chem Biol Interact 202(1-3);70-7. PMID: 23237860
Guadalupe10: Guadalupe Medina V, Almering MJ, van Maris AJ, Pronk JT (2010). "Elimination of glycerol production in anaerobic cultures of a Saccharomyces cerevisiae strain engineered to use acetic acid as an electron acceptor." Appl Environ Microbiol 76(1);190-5. PMID: 19915031
Gupta00: Gupta S, Mat-Jan F, Latifi M, Clark DP (2000). "Acetaldehyde dehydrogenase activity of the AdhE protein of Escherichia coli is inhibited by intermediates in ubiquinone synthesis." FEMS Microbiol Lett 182(1);51-5. PMID: 10612730
Harayama91: Harayama S, Rekik M, Bairoch A, Neidle EL, Ornston LN (1991). "Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xylXYZ, genes encoding benzoate dioxygenases." J Bacteriol 1991;173(23);7540-8. PMID: 1938949
He99: He Z, Spain JC (1999). "Comparison of the downstream pathways for degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45 (2-aminophenol pathway) and by Comamonas sp. JS765 (catechol pathway)." Arch Microbiol 171(5);309-16. PMID: 10382261
Kazahaya72: Kazahaya, T., Kawai, K., Yashima, S., Sasaki, Y. (1972). "Aerobic dissimilation of glucose by heterolactic bacteria III. Aldehyde dehydrogenase And alcohol dehydrogenase of Leuconostoc mesenteroides." J. Gen. Appl. Microbiol. 18:43-55.
Kessler91: Kessler D, Leibrecht I, Knappe J (1991). "Pyruvate-formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE." FEBS Lett 281(1-2);59-63. PMID: 2015910
Kukor91: Kukor JJ, Olsen RH (1991). "Genetic organization and regulation of a meta cleavage pathway for catechols produced from catabolism of toluene, benzene, phenol, and cresols by Pseudomonas pickettii PKO1." J Bacteriol 1991;173(15);4587-94. PMID: 1856161
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