This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Synonyms: ortho-nitrophenol degradation, o-nitrophenol degradation
|Superclasses:||Degradation/Utilization/Assimilation → Aromatic Compounds Degradation → Nitroaromatic Compounds Degradation → Nitrophenol Degradation → 2-Nitrophenol Degradation|
|Degradation/Utilization/Assimilation → Aromatic Compounds Degradation → Phenolic Compounds Degradation → Nitrophenol Degradation → 2-Nitrophenol Degradation|
Some taxa known to possess this pathway include : Pseudomonas putida B2
Expected Taxonomic Range: Bacteria
Nitroaromatic compounds are found in many pesticides, dyes, explosives, and solvents, and they also serve as precursors for the production of aminoaromatic derivatives. Because of their common usage, nitroaromatic compounds have become pollutants in rivers, wastewaters, groundwaters, pesticide-treated soils, and the atmosphere. 2-nitrophenol (ONP) and 2,4-dinitrophenol have been listed as priority pollutants by the U.S. Environmental Protection Agency [Callahan79].
Degradation of 2-nitrophenol by Pseudomonas has been reported several times in the literature [Simpson53, Germanier63]. A Pseudomonas putida strain that grew on 2-nitrophenol and m-nitrophenol as sole sources of carbon and nitrogen was isolated from soil by Zeyer and Kearney [Zeyer84]. Growing and resting cells degraded 2-nitrophenol and released nitrite. Experiments with a cell-free system demonstrated that the organism possessed an inducible enzyme that degraded 2-nitrophenol to nitrite and catechol [Zeyer84]. The later was further degraded via 3-oxoadipate by the ortho-cleavage system (see catechol degradation III (ortho-cleavage pathway) [Zeyer86].
Relationship Links: Eawag-BBD-Pathways:PART-OF:nphe
Callahan79: Callahan, M. A., Slimak, M. W., Gabel, N. W., May, J. P., Fowler, C. F., Freed, J. R., Jennings, P., Durfee, R. L., Whitmore, F. C., Maestri, B., Mabey, W. R., Holt, B. R., Gould, C. (1979). "Water-related environmental fate of 129 priority pollutants." EPA report-440/4-79-029b. U.S. Environmental Protection Agency, Washington, D.C.
Zeyer86: Zeyer J, Kocher HP, Timmis KN (1986). "Influence of para-substituents on the oxidative metabolism of o-nitrophenols by Pseudomonas putida B2." Appl Environ Microbiol 52(2);334-9. PMID: 3752997
Zeyer88: Zeyer J, Kocher HP (1988). "Purification and characterization of a bacterial nitrophenol oxygenase which converts ortho-nitrophenol to catechol and nitrite." J Bacteriol 170(4);1789-94. PMID: 3350791
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