This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Degradation/Utilization/Assimilation → Aromatic Compounds Degradation → Nitroaromatic Compounds Degradation → Nitrobenzene Degradation|
Some taxa known to possess this pathway include : Comamonas sp. JS765
Expected Taxonomic Range:
nitrobenzene is frequently released in the effluent from explosives manufacture as well as in the manufacture of organic chemicals and plastics. It is the primary starting material for the manufacture of aniline, a compound used in the production of polyurethane [Nishino93]. The pollution potential of nitrobenzene is very large, and it is listed among the U.S. Environmental Protection Agency priority pollutants [Callahan79].
The bacterium Comamonas sp. JS765, which was isolated from an industrial waste treatment plant in New Jersey, can utilize nitrobenzene as the sole source for carbon, nitrogen and energy [Nishino95]. nitrobenzene is converted to catechol by the enzyme nitroarene dioxygenase complex, a large complex that belongs to the family of Rieske nonheme iron oxygenases [Lessner02]. The catechol is then degraded by a meta-cleavage pathway via 2-oxopent-4-enoate to pyruvate and acetyl-CoA [He99].
Relationship Links: Eawag-BBD-Pathways:PART-OF:nb
Callahan79: Callahan, M. A., Slimak, M. W., Gabel, N. W., May, J. P., Fowler, C. F., Freed, J. R., Jennings, P., Durfee, R. L., Whitmore, F. C., Maestri, B., Mabey, W. R., Holt, B. R., Gould, C. (1979). "Water-related environmental fate of 129 priority pollutants." EPA report-440/4-79-029b. U.S. Environmental Protection Agency, Washington, D.C.
He99: He Z, Spain JC (1999). "Comparison of the downstream pathways for degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45 (2-aminophenol pathway) and by Comamonas sp. JS765 (catechol pathway)." Arch Microbiol 171(5);309-16. PMID: 10382261
Lessner02: Lessner DJ, Johnson GR, Parales RE, Spain JC, Gibson DT (2002). "Molecular characterization and substrate specificity of nitrobenzene dioxygenase from Comamonas sp. strain JS765." Appl Environ Microbiol 68(2);634-41. PMID: 11823201
Liu11c: Liu H, Wang SJ, Zhang JJ, Dai H, Tang H, Zhou NY (2011). "Patchwork assembly of nag-like nitroarene dioxygenase genes and the 3-chlorocatechol degradation cluster for evolution of the 2-chloronitrobenzene catabolism pathway in Pseudomonas stutzeri ZWLR2-1." Appl Environ Microbiol 77(13);4547-52. PMID: 21602392
Parales05: Parales RE, Huang R, Yu CL, Parales JV, Lee FK, Lessner DJ, Ivkovic-Jensen MM, Liu W, Friemann R, Ramaswamy S, Gibson DT (2005). "Purification, characterization, and crystallization of the components of the nitrobenzene and 2-nitrotoluene dioxygenase enzyme systems." Appl Environ Microbiol 71(7);3806-14. PMID: 16000792
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