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MetaCyc Pathway: inosine-5'-phosphate biosynthesis I
Inferred from experiment

Enzyme View:

Pathway diagram: inosine-5'-phosphate biosynthesis I

This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Synonyms: IMP biosynthesis I

Superclasses: BiosynthesisNucleosides and Nucleotides BiosynthesisPurine Nucleotide BiosynthesisPurine Nucleotides De Novo BiosynthesisPurine Riboucleotides De Novo BiosynthesisInosine-5'-phosphate Biosynthesis

Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655, Salmonella enterica enterica serovar Typhimurium

Expected Taxonomic Range: Bacteria

De novo biosynthesis of purines starts with the synthesis of IMP, which can be converted to all other purines.

In bacteria and archaea IMP is synthesized from 5-amino-1-(5-phospho-β-D-ribosyl)imidazole in 6 steps, while in eukarya the first two steps are replaced by a single reaction (see inosine-5'-phosphate biosynthesis II).

The first 4 steps of the bacterial pathway are catalyzed by individual enzymes, and the last two steps are catalyzed by a single multifunctional enzyme, AICAR transformylase / IMP cyclohydrolase, which is encoded by the purH gene.

In archaea the last two steps are catalyzes by two different enzymes (see inosine-5'-phosphate biosynthesis III).

Genetic studies indicate that in bacterial systems the majority of de novo purine biosynthetic genes are unlinked, but may act as a single unit of regulation controlled by the `purR' repressor protein.

Superpathways: superpathway of purine nucleotides de novo biosynthesis II, superpathway of histidine, purine, and pyrimidine biosynthesis

Variants: inosine-5'-phosphate biosynthesis II, inosine-5'-phosphate biosynthesis III

Unification Links: EcoCyc:PWY-6123

Created 13-Jan-2009 by Caspi R, SRI International


Neidhardt96: Neidhardt FC, Curtiss III R, Ingraham JL, Lin ECC, Low Jr KB, Magasanik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE "Escherichia coli and Salmonella, Cellular and Molecular Biology, Second Edition." American Society for Microbiology, Washington, D.C., 1996.

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Aiba89: Aiba A, Mizobuchi K (1989). "Nucleotide sequence analysis of genes purH and purD involved in the de novo purine nucleotide biosynthesis of Escherichia coli." J Biol Chem 1989;264(35);21239-46. PMID: 2687276

Alenin92: Alenin VV, Ostanin KV, Kostikova TR, Domkin VD, Zubova VA, Smirnov MN (1992). "[Substrate specificity of phosphoribosyl-aminoimidazole-succinocarboxamide synthetase (SAICAR-synthetase) from Saccharomyces cerevisiae yeast]." Biokhimiia 1992;57(6);845-55. PMID: 1420588

Axelrod08: Axelrod HL, McMullan D, Krishna SS, Miller MD, Elsliger MA, Abdubek P, Ambing E, Astakhova T, Carlton D, Chiu HJ, Clayton T, Duan L, Feuerhelm J, Grzechnik SK, Hale J, Han GW, Haugen J, Jaroszewski L, Jin KK, Klock HE, Knuth MW, Koesema E, Morse AT, Nigoghossian E, Okach L, Oommachen S, Paulsen J, Quijano K, Reyes R, Rife CL, van den Bedem H, Weekes D, White A, Wolf G, Xu Q, Hodgson KO, Wooley J, Deacon AM, Godzik A, Lesley SA, Wilson IA (2008). "Crystal structure of AICAR transformylase IMP cyclohydrolase (TM1249) from Thermotoga maritima at 1.88 A resolution." Proteins 71(2);1042-9. PMID: 18260100

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Bulock02: Bulock KG, Beardsley GP, Anderson KS (2002). "The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase). A surprising lack of substrate channeling." J Biol Chem 277(25);22168-74. PMID: 11948179

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Firestine94: Firestine SM, Poon SW, Mueller EJ, Stubbe J, Davisson VJ (1994). "Reactions catalyzed by 5-aminoimidazole ribonucleotide carboxylases from Escherichia coli and Gallus gallus: a case for divergent catalytic mechanisms." Biochemistry 1994;33(39);11927-34. PMID: 7918411

Flannigan90: Flannigan KA, Hennigan SH, Vogelbacker HH, Gots JS, Smith JM (1990). "Purine biosynthesis in Escherichia coli K12: structure and DNA sequence studies of the purHD locus." Mol Microbiol 1990;4(3);381-92. PMID: 2192230

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Ginder06: Ginder ND, Binkowski DJ, Fromm HJ, Honzatko RB (2006). "Nucleotide complexes of Escherichia coli phosphoribosylaminoimidazole succinocarboxamide synthetase." J Biol Chem 281(30);20680-8. PMID: 16687397

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Gots69: Gots JS, Dalal FR, Shumas SR (1969). "Genetic eparation of the inosinic acid cyclohydrolase-transformylase complex of Salmonella typhimurium." J Bacteriol 99(2);441-9. PMID: 4897111

Graupner02: Graupner M, Xu H, White RH (2002). "New class of IMP cyclohydrolases in Methanococcus jannaschii." J Bacteriol 184(5);1471-3. PMID: 11844782

Green96: Green SM, Malik T, Giles IG, Drabble WT (1996). "The purB gene of Escherichia coli K-12 is located in an operon." Microbiology 142 ( Pt 11);3219-30. PMID: 8969519

He92: He B, Smith JM, Zalkin H (1992). "Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR." J Bacteriol 1992;174(1);130-6. PMID: 1729205

Hoskins07: Hoskins AA, Morar M, Kappock TJ, Mathews II, Zaugg JB, Barder TE, Peng P, Okamoto A, Ealick SE, Stubbe J (2007). "N5-CAIR mutase: role of a CO2 binding site and substrate movement in catalysis." Biochemistry 46(10);2842-55. PMID: 17298082

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Jung10: Jung SC, Smith CL, Lee KS, Hong ME, Kweon DH, Stephanopoulos G, Jin YS (2010). "Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB." Appl Environ Microbiol 76(18);6307-9. PMID: 20675450

Showing only 20 references. To show more, press the button "Show all references".

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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