If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
Some taxa known to possess this pathway include : Methanobacterium thermoautotrophicum Marburg, Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus Delta H, Methanothermobacter thermautotrophicus Winter
Expected Taxonomic Range: Archaea
Methanothermobacter thermautotrophicus is a thermophilic methanogenic archaebacterium. The organism is an autotrophic anaerobe and can grow on a gas mixture of CO2 and H2 as its carbon and energy sources, respectively.
The first, and main route, is the reductive acetyl coenzyme A pathway. The product of this pathway, acetyl-CoA, is a central intermediate in this organism and is used for all subsequent biosynthetic pathways [Fuchs80, Jansen82].
A third CO2 molecule is incorporated by pyruvate carboxylase, which converts pyruvate to oxaloacetate [Mukhopadhyay98a]. This enzyme accepts only hydrogen carbonate molecules, but Methanothermobacter thermautotrophicus Delta H possesses a carbonic anhydrase enzyme, which can convert CO2 into hydrogen carbonate [Smith99].
Amino acid biosynthesis:
phosphoenolpyruvate leads to a gluconeogenesis pathway that ends at β-D-glucose 6-phosphate, furnishing the cell with all of the glycolysis/gluconeogenesis intermediates that are required for biosynthesis [Fuchs80],
Like other archaebacteria, the main phospholipid in Methanothermobacter thermautotrophicus membranes is unsaturated archaetidylserine. This phospholipid is synthesized from a sn-glycerol 1-phosphate backbone (produced from glycerone phosphate) and geranylgeranyl diphosphate, which is produced from acetyl-CoA.
Most of the enzymes catalyzing these pathways have not been identified or characterized. The pathways were predicted using stable isotope labeling and NMR spectroscopy experiments [Jansen82, Evans86a].
Subpathways: geranylgeranyl diphosphate biosynthesis, trans, trans-farnesyl diphosphate biosynthesis, mevalonate pathway I, superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate), archaetidylserine and archaetidylethanolamine biosynthesis, L-alanine biosynthesis II, L-aspartate biosynthesis, incomplete reductive TCA cycle, gluconeogenesis II (Methanobacterium thermoautotrophicum), reductive acetyl coenzyme A pathway, CDP-archaeol biosynthesis
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