MetaCyc Pathway: adenosine 5'-phosphoramidate biosynthesis
Inferred from experiment

Enzyme View:

Pathway diagram: adenosine 5'-phosphoramidate biosynthesis

This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Superclasses: BiosynthesisNucleosides and Nucleotides BiosynthesisPurine Nucleotide Biosynthesis

Some taxa known to possess this pathway include : Chlorella pyrenoidosa, Dictyostelium discoideum, Euglena gracilis, Hordeum vulgare, Spinacia oleracea

Expected Taxonomic Range: cellular organisms

Adenosine 5'-phosphoramidate is a natural but uncommon nucleotide that was discovered in Chlorella pyrenoidosa cells [Fankhauser81a]. The biochemistry of this compound is poorly understood.

A study that looked for the compound in cell extracts found it in a range of organisms including Chlorella pyrenoidosa, Euglena gracilis, Spinacia oleracea, Hordeum vulgare, Dictyostelium discoideum and Escherichia coli [Fankhauser81].

An enzyme that catalyzes the formation of adenosine 5'-phosphoramidate from adenosine 5'-phosphosulfate and ammonia was purified and characterized from chlorella cells [Fankhauser81].

The degradation of adenosine 5'-phosphoramidate has been studied in yellow Lupin ( Lupinus luteus) by using the nucleotide analog adenosine 5'-phosphorothioate. The study showed that the lupin adenosine 5'-phosphoramidase splits the P-S bond in adenosine 5'-phosphorothioate, similarly to the action of HINT4 proteins in Arabidopsis [Guranowski11].

Created 20-Apr-2011 by Pujar A, Boyce Thompson Institute
Revised 04-Jan-2013 by Caspi R, SRI International


Fankhauser81: Fankhauser H, Schiff JA, Garber LJ (1981). "Purification and properties of adenylyl sulphate:ammonia adenylyltransferase from Chlorella catalysing the formation of adenosine 5' -phosphoramidate from adenosine 5' -phosphosulphate and ammonia." Biochem J 195(3);545-60. PMID: 6274307

Fankhauser81a: Fankhauser H, Berkowitz GA, Schiff JA (1981). "A nucleotide with the properties of adenosine 5' phosphoramidate from Chlorella cells." Biochem Biophys Res Commun 101(2);524-32. PMID: 7306093

Guranowski11: Guranowski A, Wojdyla AM, Rydzik AM, Stepinski J, Jemielity J (2011). "Plant nucleoside 5'-phosphoramidate hydrolase; simple purification from yellow lupin (Lupinus luteus) seeds and properties of homogeneous enzyme." Acta Biochim Pol 58(1);131-6. PMID: 21403921

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 19.5 (software by SRI International) on Thu May 5, 2016, biocyc13.