MetaCyc Pathway: L-methionine biosynthesis II
Inferred from experiment

Enzyme View:

Pathway diagram: L-methionine biosynthesis II

This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Synonyms: L-methionine biosynthesis via O-phospho-homoserine

Superclasses: BiosynthesisAmino Acids BiosynthesisProteinogenic Amino Acids BiosynthesisL-methionine BiosynthesisL-methionine De Novo Biosynthesis

Some taxa known to possess this pathway include : Arabidopsis thaliana col, Solanum lycopersicum

Expected Taxonomic Range: Embryophyta

L-methionine (met) is an essential amino acid and is required for a number of important cellular functions, including the initiation of protein synthesis, the methylation of DNA, rRNA and xenobiotics, and the biosynthesis of cysteine, phospholipids and polyamines.

The nitrogen and carbon skeleton of methionine comes from homoserine, the sulfur atom from cysteine, and the methyl group from folate. Methionine biosynthesis in plants differs from that in microorganisms. O-phospho-homoserine, instead of O-succinyl-homoserine, is the intermediate converting homoserine to cystathionine. In addition, plants can utilize 5-methyltetrahydropteroyltriglutamate, S-methylmethionine, or S-adenosylmethionine as the methyl donors in converting homocysteine to methionine.

Citations: [Kim02b, Ravanel98a, Ravanel98, Ranocha00]

Superpathways: superpathway of L-lysine, L-threonine and L-methionine biosynthesis II

Unification Links: AraCyc:PWY-702

Cynthia J. Krieger on Wed Oct 15, 2003:
This pathway was formerly called methionine biosynthesis from homoserine II.

Created 05-Feb-2003 by Zhang P, TAIR


Kim02b: Kim J, Lee M, Chalam R, Martin MN, Leustek T, Boerjan W (2002). "Constitutive overexpression of cystathionine gamma-synthase in Arabidopsis leads to accumulation of soluble methionine and S-methylmethionine." Plant Physiol 2002;128(1);95-107. PMID: 11788756

Ranocha00: Ranocha P, Bourgis F, Ziemak MJ, Rhodes D, Gage DA, Hanson AD (2000). "Characterization and functional expression of cDNAs encoding methionine-sensitive and -insensitive homocysteine S-methyltransferases from Arabidopsis." J Biol Chem 275(21);15962-8. PMID: 10747987

Ravanel98: Ravanel S, Gakiere B, Job D, Douce R (1998). "The specific features of methionine biosynthesis and metabolism in plants." Proc Natl Acad Sci U S A 95(13);7805-12. PMID: 9636232

Ravanel98a: Ravanel S, Gakiere B, Job D, Douce R (1998). "Cystathionine gamma-synthase from Arabidopsis thaliana: purification and biochemical characterization of the recombinant enzyme overexpressed in Escherichia coli." Biochem J 1998;331 ( Pt 2);639-48. PMID: 9531508

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Balish66: Balish E, Shapiro SK (1966). "Cystathionine as a precursor of methionine in Escherichia coli and Aerobacter aerogenes." J Bacteriol 92(5);1331-6. PMID: 5332398

Balish67: Balish E, Shapiro SK (1967). "Methionine biosynthesis in Escherichia coli: induction and repression of methylmethionine(or adenosylmethionine):homocysteine methyltransferase." Arch Biochem Biophys 119(1);62-8. PMID: 4861151

Belfaiza86: Belfaiza J, Parsot C, Martel A, de la Tour CB, Margarita D, Cohen GN, Saint-Girons I (1986). "Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region." Proc Natl Acad Sci U S A 83(4);867-71. PMID: 3513164

Breitinger01: Breitinger U, Clausen T, Ehlert S, Huber R, Laber B, Schmidt F, Pohl E, Messerschmidt A (2001). "The three-dimensional structure of cystathionine beta-lyase from Arabidopsis and its substrate specificity." Plant Physiol 2001;126(2);631-42. PMID: 11402193

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Burr76: Burr B, Walker J, Truffa-Bachi P, Cohen GN (1976). "Homoserine kinase from Escherichia coli K12." Eur J Biochem 1976;62(3);519-26. PMID: 177283

Chassagnole01: Chassagnole C, Rais B, Quentin E, Fell DA, Mazat JP (2001). "An integrated study of threonine-pathway enzyme kinetics in Escherichia coli." Biochem J 356(Pt 2);415-23. PMID: 11368768

Clausen96: Clausen T, Huber R, Laber B, Pohlenz HD, Messerschmidt A (1996). "Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A." J Mol Biol 262(2);202-24. PMID: 8831789

Csaikl86: Csaikl U, Csaikl F (1986). "Molecular cloning and characterization of the MET6 gene of Saccharomyces cerevisiae." Gene 46(2-3);207-14. PMID: 3542720

Dwivedi82: Dwivedi CM, Ragin RC, Uren JR (1982). "Cloning, purification, and characterization of beta-cystathionase from Escherichia coli." Biochemistry 1982;21(13);3064-9. PMID: 7049234

Eichel95: Eichel J, Gonzalez JC, Hotze M, Matthews RG, Schroder J (1995). "Vitamin-B12-independent methionine synthase from a higher plant (Catharanthus roseus). Molecular characterization, regulation, heterologous expression, and enzyme properties." Eur J Biochem 230(3);1053-8. PMID: 7601135

Gakiere99: Gakiere B, Job D, Douce R, Ravanel S "Characterization of the cDNA and Gene for a Cytosolic Cobalamin-Independent Methionine Synthase in Arabidopsis thaliana (Accession No. U97200). (PGR99-115)." Plant Physiol. (1999), 120, 1206.

Grundy02: Grundy,F.J., Henkin,T.M. (2002). "Synthesis of serine, glycine, cysteine, and methionine." in Sonenshein,A.L., Hoch,J.A. and Losick,R. (eds), Bacillus subtilis and its Relatives: From Genes to Cells. American Society for Microbiology, Washington, DC, pp. 245254.

Guest64: Guest JR, Friedman S, Foster MA, Tejerina G, Woods DD (1964). "Transfer of the methyl group from N5-methyltetrahydrofolates to homocysteine in Escherichia coli." Biochem J 92(3);497-504. PMID: 5319972

Hansen00: Hansen J, Johannesen PF (2000). "Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae." Mol Gen Genet 263(3);535-42. PMID: 10821189

Hansen97: Hansen J, Muldbjerg M, Cherest H, Surdin-Kerjan Y (1997). "Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes." FEBS Lett 401(1);20-4. PMID: 9003798

Huo96: Huo X, Viola RE (1996). "Substrate specificity and identification of functional groups of homoserine kinase from Escherichia coli." Biochemistry 35(50);16180-5. PMID: 8973190

Huo96a: Huo X, Viola RE (1996). "Functional group characterization of homoserine kinase from Escherichia coli." Arch Biochem Biophys 330(2);373-9. PMID: 8660667

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Lee99: Lee M, Leustek T (1999). "Identification of the gene encoding homoserine kinase from Arabidopsis thaliana and characterization of the recombinant enzyme derived from the gene." Arch Biochem Biophys 1999;372(1);135-42. PMID: 10562426

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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