MetaCyc Pathway: L-homocysteine and L-cysteine interconversion
Inferred from experiment

Pathway diagram: L-homocysteine and L-cysteine interconversion

This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Synonyms: transsulfuration pathway

Superclasses: Activation/Inactivation/InterconversionInterconversions
BiosynthesisAmino Acids BiosynthesisProteinogenic Amino Acids BiosynthesisL-cysteine Biosynthesis

Some taxa known to possess this pathway include : Saccharomyces cerevisiae

Expected Taxonomic Range: Fungi, Viridiplantae

Trans-sulfuration pathways allow the interconversion of L-homocysteine and L-cysteine via L-cystathionine. In contrast to enteric bacteria and mammals, Saccharomyces cerevisiae has two transsulfuration pathways employing two separate sets of enzymes [Thomas97].

Superpathways: L-cysteine biosynthesis IV (fungi), superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)

Variants: L-cysteine biosynthesis I, L-cysteine biosynthesis II (tRNA-dependent), L-cysteine biosynthesis III (from L-homocysteine), L-cysteine biosynthesis V, superpathway of L-cysteine biosynthesis (mammalian)

Created 22-May-2001 by Pellegrini-Toole A, Marine Biological Laboratory


Thomas97: Thomas D, Surdin-Kerjan Y (1997). "Metabolism of sulfur amino acids in Saccharomyces cerevisiae." Microbiol Mol Biol Rev 61(4);503-32. PMID: 9409150

Vermeij99: Vermeij P, Kertesz MA (1999). "Pathways of assimilative sulfur metabolism in Pseudomonas putida." J Bacteriol 181(18);5833-7. PMID: 10482527

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Awata89: Awata S, Nakayama K, Suzuki I, Kodama H (1989). "Effect of cysteine on the inactivation of cystathionine gamma-lyase by D,L-propargylglycine." Acta Med Okayama 43(6);329-35. PMID: 2624140

Barton93: Barton AB, Kaback DB, Clark MW, Keng T, Ouellette BF, Storms RK, Zeng B, Zhong W, Fortin N, Delaney S (1993). "Physical localization of yeast CYS3, a gene whose product resembles the rat gamma-cystathionase and Escherichia coli cystathionine gamma-synthase enzymes." Yeast 9(4);363-9. PMID: 8511966

Belfaiza86: Belfaiza J, Parsot C, Martel A, de la Tour CB, Margarita D, Cohen GN, Saint-Girons I (1986). "Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region." Proc Natl Acad Sci U S A 83(4);867-71. PMID: 3513164

Cherest92: Cherest H, Surdin-Kerjan Y (1992). "Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway." Genetics 130(1);51-8. PMID: 1732168

Cherest93: Cherest H, Thomas D, Surdin-Kerjan Y (1993). "Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment." J Bacteriol 1993;175(17);5366-74. PMID: 8366024

Clausen96: Clausen T, Huber R, Laber B, Pohlenz HD, Messerschmidt A (1996). "Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A." J Mol Biol 262(2);202-24. PMID: 8831789

Dwivedi82: Dwivedi CM, Ragin RC, Uren JR (1982). "Cloning, purification, and characterization of beta-cystathionase from Escherichia coli." Biochemistry 1982;21(13);3064-9. PMID: 7049234

Grobbelaar58: Grobbelaar, N, Steward FC (1958). "O-acetylhomoserine in Pisum." Nature 182(4646);1358-9. PMID: 13600320

Hansen00: Hansen J, Johannesen PF (2000). "Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae." Mol Gen Genet 263(3);535-42. PMID: 10821189

Jhee00a: Jhee KH, McPhie P, Miles EW (2000). "Yeast cystathionine beta-synthase is a pyridoxal phosphate enzyme but, unlike the human enzyme, is not a heme protein." J Biol Chem 275(16);11541-4. PMID: 10766767

Kimura71: Kimura H, Nakagawa H (1971). "Studies on cystathionine synthetase characteristics of purified rat liver enzyme." J Biochem (Tokyo) 69(4);711-23. PMID: 5572807

Kraus78: Kraus J, Packman S, Fowler B, Rosenberg LE (1978). "Purification and properties of cystathionine beta-synthase from human liver. Evidence for identical subunits." J Biol Chem 253(18);6523-8. PMID: 681363

Kruger94: Kruger WD, Cox DR (1994). "A yeast system for expression of human cystathionine beta-synthase: structural and functional conservation of the human and yeast genes." Proc Natl Acad Sci U S A 91(14);6614-8. PMID: 8022826

Lambrecht12: Lambrecht JA, Flynn JM, Downs DM (2012). "Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions." J Biol Chem 287(5);3454-61. PMID: 22094463

Latendresse13: Latendresse M. (2013). "Computing Gibbs Free Energy of Compounds and Reactions in MetaCyc."

Lodha10: Lodha PH, Jaworski AF, Aitken SM (2010). "Characterization of site-directed mutants of residues R58, R59, D116, W340 and R372 in the active site of E. coli cystathionine beta-lyase." Protein Sci 19(3);383-91. PMID: 20014435

Maclean00: Maclean KN, Janosik M, Oliveriusova J, Kery V, Kraus JP (2000). "Transsulfuration in Saccharomyces cerevisiae is not dependent on heme: purification and characterization of recombinant yeast cystathionine beta-synthase." J Inorg Biochem 81(3);161-71. PMID: 11051561

Matsuo58: Matsuo, Y, Greenberg, DM (1958). "A crystalline enzyme that cleaves homoserine and cystathionine. I. Isolation procedure and some physicochemical properties." J Biol Chem 230(2);545-60. PMID: 13525371

Matsuo59: Matsuo, Y, Greenberg, DM (1959). "A crystalline enzyme that cleaves homoserine and cystathionine. III. Coenzyme resolution, activators, and inhibitors." J Biol Chem 234(3);507-15. PMID: 13641250

Matsuo59a: Matsuo, Y., Greenberg, D.M. (1959). "A crystalline enzyme that cleaves homoserine and cystathionine. IV. Mechanism of action, reversibility, and substrate specificity." J Biol Chem 234(3):516-9. PMID: 13641251

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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