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MetaCyc Pathway: superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)

Pathway diagram: superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)

If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.

Superclasses: BiosynthesisAmino Acids Biosynthesis

Some taxa known to possess this pathway include : Saccharomyces cerevisiae

Expected Taxonomic Range: Fungi

The yeast Saccharomyces cerevisiae can directly assimilate inorganic sulfur for the biosynthesis of sulfur-containing amino acids. Detailed description of the pathway converting sulfate to hydrogen sulfide is provided in the pathway sulfate reduction I (assimilatory).

L-homoserine is biosynthesized from L-aspartate, which in turn is synthesized from oxaloacetate, a TCA cycle intermediate. Homoserine is activated through esterification to form the O-acetyl ester by the enzyme homoserine O-acetyltransferase. hydrogen sulfide, which is the end result of sulfate assimilation, reacts with O-acetyl-L-homoserine, replacing the acetyl group and forming L-homocysteine.

Finally, L-homocysteine can be converted to L-cysteine via L-cystathionine by a reversible transsulfuration pathway. In contrast to enteric bacteria and mammals, Saccharomyces cerevisiae has two transsulfuration pathways employing two separate sets of enzymes [Thomas97].

Subpathways: sulfate reduction I (assimilatory), L-homocysteine biosynthesis, L-homocysteine and L-cysteine interconversion, sulfate activation for sulfonation

Revised 15-Aug-2007 by Caspi R, SRI International


Thomas97: Thomas D, Surdin-Kerjan Y (1997). "Metabolism of sulfur amino acids in Saccharomyces cerevisiae." Microbiol Mol Biol Rev 61(4);503-32. PMID: 9409150

Other References Related to Enzymes, Genes, Subpathways, and Substrates of this Pathway

Abola99: Abola AP, Willits MG, Wang RC, Long SR (1999). "Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae." J Bacteriol 181(17);5280-7. PMID: 10464198

Awata89: Awata S, Nakayama K, Suzuki I, Kodama H (1989). "Effect of cysteine on the inactivation of cystathionine gamma-lyase by D,L-propargylglycine." Acta Med Okayama 43(6);329-35. PMID: 2624140

Bairoch93: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

Bairoch93a: Bairoch A, Boeckmann B (1993). "The SWISS-PROT protein sequence data bank, recent developments." Nucleic Acids Res. 21:3093-3096. PMID: 8332529

Baroni86: Baroni M, Livian S, Martegani E, Alberghina L (1986). "Molecular cloning and regulation of the expression of the MET2 gene of Saccharomyces cerevisiae." Gene 46(1);71-8. PMID: 3542717

Barton93: Barton AB, Kaback DB, Clark MW, Keng T, Ouellette BF, Storms RK, Zeng B, Zhong W, Fortin N, Delaney S (1993). "Physical localization of yeast CYS3, a gene whose product resembles the rat gamma-cystathionase and Escherichia coli cystathionine gamma-synthase enzymes." Yeast 9(4);363-9. PMID: 8511966

Belfaiza86: Belfaiza J, Parsot C, Martel A, de la Tour CB, Margarita D, Cohen GN, Saint-Girons I (1986). "Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region." Proc Natl Acad Sci U S A 83(4);867-71. PMID: 3513164

Belfaiza98: Belfaiza J, Martel A, Margarita D, Saint Girons I (1998). "Direct sulfhydrylation for methionine biosynthesis in Leptospira meyeri." J Bacteriol 180(2);250-5. PMID: 9440513

Berendt95: Berendt U, Haverkamp T, Prior A, Schwenn JD (1995). "Reaction mechanism of thioredoxin: 3'-phospho-adenylylsulfate reductase investigated by site-directed mutagenesis." Eur J Biochem 1995;233(1);347-56. PMID: 7588765

Berndt04: Berndt C, Lillig CH, Wollenberg M, Bill E, Mansilla MC, de Mendoza D, Seidler A, Schwenn JD (2004). "Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis." J Biol Chem 279(9);7850-5. PMID: 14627706

Bourhy97: Bourhy P, Martel A, Margarita D, Saint Girons I, Belfaiza J (1997). "Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited." J Bacteriol 179(13);4396-8. PMID: 9209059

BRENDA14: BRENDA team (2014). Imported from BRENDA version existing on Aug 2014.

Cherest92: Cherest H, Surdin-Kerjan Y (1992). "Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway." Genetics 130(1);51-8. PMID: 1732168

Cherest93: Cherest H, Thomas D, Surdin-Kerjan Y (1993). "Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment." J Bacteriol 1993;175(17);5366-74. PMID: 8366024

Clausen96: Clausen T, Huber R, Laber B, Pohlenz HD, Messerschmidt A (1996). "Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A." J Mol Biol 262(2);202-24. PMID: 8831789

Coves93: Coves J, Niviere V, Eschenbrenner M, Fontecave M (1993). "NADPH-sulfite reductase from Escherichia coli. A flavin reductase participating in the generation of the free radical of ribonucleotide reductase." J Biol Chem 1993;268(25);18604-9. PMID: 8360156

Dahl90: Dahl, C., Koch, H., Keuken, O., Trueper, H.G. (1990). "Purification and characterization of ATP sulfurylase from the extremely thermophilic archaebacterial sulfate-reducer, Archaeoglobus fulgidus." FEMS Microbiol. Let. 67: 27-32.

DAndrea87: D'Andrea R, Surdin-Kerjan Y, Pure G, Cherest H (1987). "Molecular genetics of met 17 and met 25 mutants of Saccharomyces cerevisiae: intragenic complementation between mutations of a single structural gene." Mol Gen Genet 207(1);165-70. PMID: 3299001

Dwivedi82: Dwivedi CM, Ragin RC, Uren JR (1982). "Cloning, purification, and characterization of beta-cystathionase from Escherichia coli." Biochemistry 1982;21(13);3064-9. PMID: 7049234

Dyson90: Dyson HJ, Gippert GP, Case DA, Holmgren A, Wright PE (1990). "Three-dimensional solution structure of the reduced form of Escherichia coli thioredoxin determined by nuclear magnetic resonance spectroscopy." Biochemistry 1990;29(17);4129-36. PMID: 2193685

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Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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