If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Biosynthesis → Amino Acids Biosynthesis|
Some taxa known to possess this pathway include : Saccharomyces cerevisiae
Expected Taxonomic Range: Fungi
The yeast Saccharomyces cerevisiae can directly assimilate inorganic sulfur for the biosynthesis of sulfur-containing amino acids. Detailed description of the pathway converting sulfate to hydrogen sulfide is provided in the pathway sulfate reduction I (assimilatory).
L-homoserine is biosynthesized from L-aspartate, which in turn is synthesized from oxaloacetate, a TCA cycle intermediate. Homoserine is activated through esterification to form the O-acetyl ester by the enzyme homoserine O-acetyltransferase. hydrogen sulfide, which is the end result of sulfate assimilation, reacts with O-acetyl-L-homoserine, replacing the acetyl group and forming L-homocysteine.
Finally, L-homocysteine can be converted to L-cysteine via L-cystathionine by a reversible transsulfuration pathway. In contrast to enteric bacteria and mammals, Saccharomyces cerevisiae has two transsulfuration pathways employing two separate sets of enzymes [Thomas97].
Abola99: Abola AP, Willits MG, Wang RC, Long SR (1999). "Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae." J Bacteriol 181(17);5280-7. PMID: 10464198
Barton93: Barton AB, Kaback DB, Clark MW, Keng T, Ouellette BF, Storms RK, Zeng B, Zhong W, Fortin N, Delaney S (1993). "Physical localization of yeast CYS3, a gene whose product resembles the rat gamma-cystathionase and Escherichia coli cystathionine gamma-synthase enzymes." Yeast 9(4);363-9. PMID: 8511966
Belfaiza86: Belfaiza J, Parsot C, Martel A, de la Tour CB, Margarita D, Cohen GN, Saint-Girons I (1986). "Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region." Proc Natl Acad Sci U S A 83(4);867-71. PMID: 3513164
Berendt95: Berendt U, Haverkamp T, Prior A, Schwenn JD (1995). "Reaction mechanism of thioredoxin: 3'-phospho-adenylylsulfate reductase investigated by site-directed mutagenesis." Eur J Biochem 1995;233(1);347-56. PMID: 7588765
Berndt04: Berndt C, Lillig CH, Wollenberg M, Bill E, Mansilla MC, de Mendoza D, Seidler A, Schwenn JD (2004). "Characterization and reconstitution of a 4Fe-4S adenylyl sulfate/phosphoadenylyl sulfate reductase from Bacillus subtilis." J Biol Chem 279(9);7850-5. PMID: 14627706
Bourhy97: Bourhy P, Martel A, Margarita D, Saint Girons I, Belfaiza J (1997). "Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited." J Bacteriol 179(13);4396-8. PMID: 9209059
Cherest92: Cherest H, Surdin-Kerjan Y (1992). "Genetic analysis of a new mutation conferring cysteine auxotrophy in Saccharomyces cerevisiae: updating of the sulfur metabolism pathway." Genetics 130(1);51-8. PMID: 1732168
Cherest93: Cherest H, Thomas D, Surdin-Kerjan Y (1993). "Cysteine biosynthesis in Saccharomyces cerevisiae occurs through the transsulfuration pathway which has been built up by enzyme recruitment." J Bacteriol 1993;175(17);5366-74. PMID: 8366024
Clausen96: Clausen T, Huber R, Laber B, Pohlenz HD, Messerschmidt A (1996). "Crystal structure of the pyridoxal-5'-phosphate dependent cystathionine beta-lyase from Escherichia coli at 1.83 A." J Mol Biol 262(2);202-24. PMID: 8831789
Coves93: Coves J, Niviere V, Eschenbrenner M, Fontecave M (1993). "NADPH-sulfite reductase from Escherichia coli. A flavin reductase participating in the generation of the free radical of ribonucleotide reductase." J Biol Chem 1993;268(25);18604-9. PMID: 8360156
Dahl90: Dahl, C., Koch, H., Keuken, O., Trueper, H.G. (1990). "Purification and characterization of ATP sulfurylase from the extremely thermophilic archaebacterial sulfate-reducer, Archaeoglobus fulgidus." FEMS Microbiol. Let. 67: 27-32.
DAndrea87: D'Andrea R, Surdin-Kerjan Y, Pure G, Cherest H (1987). "Molecular genetics of met 17 and met 25 mutants of Saccharomyces cerevisiae: intragenic complementation between mutations of a single structural gene." Mol Gen Genet 207(1);165-70. PMID: 3299001
Dyson90: Dyson HJ, Gippert GP, Case DA, Holmgren A, Wright PE (1990). "Three-dimensional solution structure of the reduced form of Escherichia coli thioredoxin determined by nuclear magnetic resonance spectroscopy." Biochemistry 1990;29(17);4129-36. PMID: 2193685
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