This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Biosynthesis → Amino Acids Biosynthesis → Individual Amino Acids Biosynthesis → Asparagine Biosynthesis|
Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655
L-asparagine is synthesized in Escherichia coli from L-aspartate by either of two reactions, utilizing either L-glutamine or ammonia as the amino group donor. Both reactions are ATP driven and yield AMP and pyrophosphate.
Null mutations in both encoding genes, asnA and asnB, result in asparagine auxotrophy, as does a null mutation only in asnB under conditions of ammonia-limited growth. A null mutation only in asnA has no observable phenotype.
The only known role of asparagine in the metabolism of Escherichia coli is as a constituent of protein.
Review: Reitzer, L. (2004) "Biosynthesis of Glutamate, Aspartate, Asparagine, L-Alanine, and D-Alanine." EcoSal 220.127.116.11 [ECOSAL].
Unification Links: EcoCyc:PWY0-1325
Boehlein94: Boehlein SK, Richards NG, Schuster SM (1994). "Glutamine-dependent nitrogen transfer in Escherichia coli asparagine synthetase B. Searching for the catalytic triad." J Biol Chem 269(10);7450-7. PMID: 7907328
Burland93: Burland V, Plunkett G, Daniels DL, Blattner FR (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 1993;16(3);551-61. PMID: 7686882
Cedar69: Cedar H, Schwartz JH (1969). "The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products." J Biol Chem 1969;244(15);4112-21. PMID: 4895361
Charron04: Charron C, Roy H, Blaise M, Giege R, Kern D (2004). "Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase." Acta Crystallogr D Biol Crystallogr 60(Pt 4);767-9. PMID: 15039580
Ciustea05: Ciustea M, Gutierrez JA, Abbatiello SE, Eyler JR, Richards NG (2005). "Efficient expression, purification, and characterization of C-terminally tagged, recombinant human asparagine synthetase." Arch Biochem Biophys 440(1);18-27. PMID: 16023613
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Humbert80: Humbert R, Simoni RD (1980). "Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli." J Bacteriol 1980;142(1);212-20. PMID: 6102982
Larsen99: Larsen TM, Boehlein SK, Schuster SM, Richards NG, Thoden JB, Holden HM, Rayment I (1999). "Three-dimensional structure of Escherichia coli asparagine synthetase B: a short journey from substrate to product." Biochemistry 1999;38(49);16146-57. PMID: 10587437
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