This view shows enzymes only for those organisms listed below, in the list of taxa known to possess the pathway. If an enzyme name is shown in bold, there is experimental evidence for this enzymatic activity.
|Superclasses:||Degradation/Utilization/Assimilation → Fatty Acid and Lipids Degradation → Fatty Acids Degradation|
Some taxa known to possess this pathway include : Escherichia coli K-12 substr. MG1655
Expected Taxonomic Range:
Oleate can serve as the sole source of carbon for Escherichia coli. Oleate is an unsaturated fatty acid and first undergoes two rounds of β-oxidation (see fatty acid β-oxidation I), producing 2-trans,5-cis-tetradecadienoyl-CoA. The original unsaturated bond is now located in the 5 position.
While the majority of 2-trans,5-cis-tetradecadienoyl-CoA is completely degraded by further rounds of β-oxidation, approximately 10% of the substrate enters a side pathway due to the production of a 3,5-unsaturated fatty acid, 3-trans,5-cis-tetradecadienoyl-CoA, from the 2,5-unsaturated 2-trans,5-cis-tetradecadienoyl-CoA by the enoyl-CoA isomerase activity of FadB. 3-trans,5-cis-tetradecadienoyl-CoA can not be further metabolized by Escherichia coli, since the organism lacks a Delta;3,5,Δ2,4-dienoyl-CoA isomerase activity. Thioesterase III liberates the CoA moiety from 3-trans,5-cis-tetradecadienoyl-CoA by hydrolysis, thus avoiding the production of a dead-end compound that traps CoA. The resulting (3E,5Z)-tetradecadienoate is secreted into the growth medium [Ren04, Nie08a].
Review: Clark, D.P. and Cronan, J.E., Two-Carbon Compounds and Fatty Acids as Carbon Sources, EcoSal Module 3.4.4 [ECOSAL]
Unification Links: EcoCyc:PWY0-1337
Nie08a: Nie L, Ren Y, Schulz H (2008). "Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation." Biochemistry 47(29);7744-51. PMID: 18576672
Ren04: Ren Y, Aguirre J, Ntamack AG, Chu C, Schulz H (2004). "An alternative pathway of oleate beta-oxidation in Escherichia coli involving the hydrolysis of a dead end intermediate by a thioesterase." J Biol Chem 279(12);11042-50. PMID: 14707139
DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114
Geisbrecht98: Geisbrecht BV, Zhu D, Schulz K, Nau K, Morrell JC, Geraghty M, Schulz H, Erdmann R, Gould SJ (1998). "Molecular characterization of Saccharomyces cerevisiae Delta3, Delta2-enoyl-CoA isomerase." J Biol Chem 273(50);33184-91. PMID: 9837886
Gurvitz98: Gurvitz A, Mursula AM, Firzinger A, Hamilton B, Kilpelainen SH, Hartig A, Ruis H, Hiltunen JK, Rottensteiner H (1998). "Peroxisomal Delta3-cis-Delta2-trans-enoyl-CoA isomerase encoded by ECI1 is required for growth of the yeast Saccharomyces cerevisiae on unsaturated fatty acids." J Biol Chem 273(47);31366-74. PMID: 9813046
He96: He XY, Yang SY (1996). "Histidine-450 is the catalytic residue of L-3-hydroxyacyl coenzyme A dehydrogenase associated with the large alpha-subunit of the multienzyme complex of fatty acid oxidation from Escherichia coli." Biochemistry 1996;35(29);9625-30. PMID: 8755745
Kal00: Kal AJ, Hettema EH, van den Berg M, Koerkamp MG, van Ijlst L, Distel B, Tabak HF (2000). "In silicio search for genes encoding peroxisomal proteins in Saccharomyces cerevisiae." Cell Biochem Biophys 32 Spring;1-8. PMID: 11330035
Maeda06: Maeda I, Delessert S, Hasegawa S, Seto Y, Zuber S, Poirier Y (2006). "The peroxisomal Acyl-CoA thioesterase Pte1p from Saccharomyces cerevisiae is required for efficient degradation of short straight chain and branched chain fatty acids." J Biol Chem 281(17);11729-35. PMID: 16490786
Mursula04: Mursula AM, Hiltunen JK, Wierenga RK (2004). "Structural studies on delta(3)-delta(2)-enoyl-CoA isomerase: the variable mode of assembly of the trimeric disks of the crotonase superfamily." FEBS Lett 557(1-3);81-7. PMID: 14741345
Nie08: Nie L, Ren Y, Janakiraman A, Smith S, Schulz H (2008). "A novel paradigm of fatty acid beta-oxidation exemplified by the thioesterase-dependent partial degradation of conjugated linoleic acid that fully supports growth of Escherichia coli." Biochemistry 47(36);9618-26. PMID: 18702504
Ntamack09: Ntamack AG, Karpichev IV, Gould SJ, Small GM, Schulz H (2009). "Oleate beta-oxidation in yeast involves thioesterase but not Yor180c protein that is not a dienoyl-CoA isomerase." Biochim Biophys Acta 1791(5);371-8. PMID: 19830908
Pramanik79: Pramanik A, Pawar S, Antonian E, Schulz H (1979). "Five different enzymatic activities are associated with the multienzyme complex of fatty acid oxidation from Escherichia coli." J Bacteriol 1979;137(1);469-73. PMID: 368024
Rubio06: Rubio S, Larson TR, Gonzalez-Guzman M, Alejandro S, Graham IA, Serrano R, Rodriguez PL (2006). "An Arabidopsis mutant impaired in coenzyme A biosynthesis is sugar dependent for seedling establishment." Plant Physiol 140(3);830-43. PMID: 16415216
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