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MetaCyc Polypeptide: Photosystem II reaction center protein M

Gene: psbM Accession Number: CHRECP026 (MetaCyc)

Species: Chlamydomonas reinhardtii

Component of:
CP43-less PS II core monomer (summary available)
PS II core monomer (summary available)
PS II core monomer (complete)
PS II core dimer (extended summary available)
photosystem II (extended summary available)

Citations: [Higgs98a, Maul02]

Locations: chloroplast thylakoid membrane, chloroplast

Map Position: [64,197 <- 64,301]

Molecular Weight of Polypeptide: 3.76 kD (from nucleotide sequence)

Unification Links: Entrez-gene:2716975 , Protein Model Portal:P92277 , RefSeq:NP_958382.1 , SMR:P92277 , UniProt:P92277

Relationship Links: InterPro:IN-FAMILY:IPR007826 , Pfam:IN-FAMILY:PF05151 , RefSeq:PART-OF:NC_005353.1

Gene-Reaction Schematic: ?

GO Terms:

Cellular Component: GO:0009507 - chloroplast
GO:0042651 - thylakoid membrane

MultiFun Terms: metabolism energy production/transport

Credits:
Revised 29-Apr-2011 by Weerasinghe D


Subunit of: CP43-less PS II core monomer

Species: Chlamydomonas reinhardtii

Subunit composition of CP43-less PS II core monomer = [PsbT][PsbR][PsbM][PsbL][PsbJ][PsbH][(PsbB)([PsbA][(PsbE)(PsbD)])]
         Photosystem II reaction center protein T = PsbT (summary available)
         10 kDa photosystem II polypeptide = PsbR (summary available)
         Photosystem II reaction center protein M = PsbM
         Photosystem II reaction center protein L = PsbL
         Photosystem II reaction center protein J = PsbJ (summary available)
         Photosystem II reaction center protein H = PsbH
         PS II RC - CP47 complex = (PsbB)([PsbA][(PsbE)(PsbD)]) (summary available)
                 Photosystem II CP47 chlorophyll apoprotein = PsbB (summary available)
                 PS II RC = (PsbA)([PsbE][PsbD])
                         Photosystem Q(B) protein = PsbA (summary available)
                         cytb559-D2 complex = (PsbE)(PsbD) (summary available)
                                 cytochrome b-559 α-subunit = PsbE (summary available)
                                 Photosystem II D2 protein = PsbD (summary available)

Component of: PS II core monomer (summary available)

Summary:
After the assembly of CP47, the low-molecular mass (LMM) subunits PsbH, L, M, T, R, and J associate with the growing PS II complex [Rokka05]. The LMM subunits are thought to stabilize the D1/D2/Cytb559/CP47 subassembly of PS II. The subunits PsbL, M, and T are located in the monomer-monomer interphase and therefore are also crucial for the dimerization of PS II [Loll05], whereas PsbR and PsbJ have been shown to be essential for the stable assembly of the oxygen-evolving complex (OEC).

Locations: chloroplast

GO Terms:

Cellular Component: GO:0009507 - chloroplast

Credits:
Created 12-Jan-2010 by May P , MPI-MP


Subunit of: PS II core monomer

Species: Chlamydomonas reinhardtii

Subunit composition of PS II core monomer = [PsbK][PsbC][(PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])])]
         Photosystem II reaction center protein K = PsbK (summary available)
         CP43 = PsbC (summary available)
         CP43-less PS II core monomer = (PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])]) (summary available)
                 Photosystem II reaction center protein T = PsbT (summary available)
                 10 kDa photosystem II polypeptide = PsbR (summary available)
                 Photosystem II reaction center protein M = PsbM
                 Photosystem II reaction center protein L = PsbL
                 Photosystem II reaction center protein J = PsbJ (summary available)
                 Photosystem II reaction center protein H = PsbH
                 PS II RC - CP47 complex = (PsbB)([PsbA][(PsbE)(PsbD)]) (summary available)
                         Photosystem II CP47 chlorophyll apoprotein = PsbB (summary available)
                         PS II RC = (PsbA)([PsbE][PsbD])
                                 Photosystem Q(B) protein = PsbA (summary available)
                                 cytb559-D2 complex = (PsbE)(PsbD) (summary available)
                                         cytochrome b-559 α-subunit = PsbE (summary available)
                                         Photosystem II D2 protein = PsbD (summary available)

Component of: PS II core monomer (complete)

Summary:
The subsequent assembly steps involve the association of the core antenna protein CP43 and the LMM subunit PsbK [Rokka05]. PsbK is tightly bound to CP43, and the assembly of PsbK was shown to occur only in presence of CP43 [Sugimoto03].

Locations: chloroplast

GO Terms:

Cellular Component: GO:0009507 - chloroplast

Credits:
Created 12-Jan-2010 by May P , MPI-MP


Subunit of: PS II core monomer (complete)

Species: Chlamydomonas reinhardtii

Subunit composition of PS II core monomer (complete) = [(PsbK)(PsbC)([PsbT][PsbR][PsbM][PsbL][PsbJ][PsbH][(PsbB)([PsbA][(PsbE)(PsbD)])])][PSBW][PsbZ][PSBO]
         PS II core monomer = (PsbK)(PsbC)([PsbT][PsbR][PsbM][PsbL][PsbJ][PsbH][(PsbB)([PsbA][(PsbE)(PsbD)])]) (summary available)
                 Photosystem II reaction center protein K = PsbK (summary available)
                 CP43 = PsbC (summary available)
                 CP43-less PS II core monomer = (PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])]) (summary available)
                         Photosystem II reaction center protein T = PsbT (summary available)
                         10 kDa photosystem II polypeptide = PsbR (summary available)
                         Photosystem II reaction center protein M = PsbM
                         Photosystem II reaction center protein L = PsbL
                         Photosystem II reaction center protein J = PsbJ (summary available)
                         Photosystem II reaction center protein H = PsbH
                         PS II RC - CP47 complex = (PsbB)([PsbA][(PsbE)(PsbD)]) (summary available)
                                 Photosystem II CP47 chlorophyll apoprotein = PsbB (summary available)
                                 PS II RC = (PsbA)([PsbE][PsbD])
                                         Photosystem Q(B) protein = PsbA (summary available)
                                         cytb559-D2 complex = (PsbE)(PsbD) (summary available)
                                                 cytochrome b-559 α-subunit = PsbE (summary available)
                                                 Photosystem II D2 protein = PsbD (summary available)
         Photosystem II reaction center W protein = PSBW
         Photosystem II reaction center protein Z = PsbZ (summary available)
         Oxygen-evolving enhancer protein 1 = PSBO (summary available)

Component of: PS II core dimer (extended summary available)

Locations: chloroplast

GO Terms:

Cellular Component: GO:0009507 - chloroplast

Credits:
Created 12-Jan-2010 by May P , MPI-MP


Subunit of: PS II core dimer

Species: Chlamydomonas reinhardtii

Subunit composition of PS II core dimer = [([PsbK][PsbC][(PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])])])(PSBW)(PsbZ)(PSBO)]2
         PS II core monomer (complete) = ([PsbK][PsbC][(PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])])])(PSBW)(PsbZ)(PSBO)
                 PS II core monomer = (PsbK)(PsbC)([PsbT][PsbR][PsbM][PsbL][PsbJ][PsbH][(PsbB)([PsbA][(PsbE)(PsbD)])]) (summary available)
                         Photosystem II reaction center protein K = PsbK (summary available)
                         CP43 = PsbC (summary available)
                         CP43-less PS II core monomer = (PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])]) (summary available)
                                 Photosystem II reaction center protein T = PsbT (summary available)
                                 10 kDa photosystem II polypeptide = PsbR (summary available)
                                 Photosystem II reaction center protein M = PsbM
                                 Photosystem II reaction center protein L = PsbL
                                 Photosystem II reaction center protein J = PsbJ (summary available)
                                 Photosystem II reaction center protein H = PsbH
                                 PS II RC - CP47 complex = (PsbB)([PsbA][(PsbE)(PsbD)]) (summary available)
                                         Photosystem II CP47 chlorophyll apoprotein = PsbB (summary available)
                                         PS II RC = (PsbA)([PsbE][PsbD])
                                                 Photosystem Q(B) protein = PsbA (summary available)
                                                 cytb559-D2 complex = (PsbE)(PsbD) (summary available)
                                                         cytochrome b-559 α-subunit = PsbE (summary available)
                                                         Photosystem II D2 protein = PsbD (summary available)
                 Photosystem II reaction center W protein = PSBW
                 Photosystem II reaction center protein Z = PsbZ (summary available)
                 Oxygen-evolving enhancer protein 1 = PSBO (summary available)

Component of: photosystem II (extended summary available)

Summary:
The PSII complex is composed of over 25 different proteins [Nield00]. The core of this multisubunit complex is composed of the D1 and D2 proteins which make up the reaction center [Barber02]. The manganese cluster, which catalyzes the oxidation of water, may be ligated to the lumenal side of the D1 protein [Nield00][Nixon94].

The chlorophyll a binding proteins CP47 and CP43 that transfer excitation energy to the reaction center surround the D1 and D2 proteins [Nield00]. Both proteins have six transmembrane helices.

Higher plants and green algae have an additional outer light-harvesting system composed of proteins that bind both chlorophyll a and b, of which there are six types (Lhcb proteins). The majority of the light-harvesting systems are made up of Lhcb13 and are known as light-harvesting complex II (LHCII) which is organized as trimers. The other chlorophyll a/b-binding proteins, Lhcb4, Lhcb5, and Lhcb6, also known as CP29, CP26, and CP24, respectively, bind less chlorophyll b than LHCII and are thought to exist as monomers [Teramoto01].

Locations: chloroplast

GO Terms:

Cellular Component: GO:0009507 - chloroplast

Credits:
Created 12-Jan-2010 by May P , MPI-MP
Revised 17-May-2011 by Weerasinghe D


Subunit of: photosystem II

Synonyms: PSII complex, PSII-LHCII supercomplex

Species: Chlamydomonas reinhardtii

Subunit composition of photosystem II = [LHCBM9][LHCBM8][LHCBM7][LHCBM6][LHCBM5][LHCBM4][LHCBM3][LHCBM2][LHCBM1][LHCB5][LHCB4][(PSBQ)(PSBP1)(PSBO)][([(PsbK)(PsbC)([PsbT][PsbR][PsbM][PsbL][PsbJ][PsbH][(PsbB)([PsbA][(PsbE)(PsbD)])])][PSBW][PsbZ][PSBO])2]
         Chlorophyll a-b binding protein of LHCII = LHCBM9 (summary available)
         Chlorophyll a-b binding protein of LHCII = LHCBM8 (summary available)
         Major light-harvesting complex II protein m7 = LHCBM7 (summary available)
         Chloropyll a-b binding protein of LHCII type I = LHCBM6 (summary available)
         Chlorophyll a-b binding protein of LHCII = LHCBM5 (summary available)
         Chloropyll a-b binding protein of LHCII = LHCBM4 (summary available)
         Light-harvesting chlorophyll-a/b binding protein LhcII-1.3 = LHCBM3 (summary available)
         Light-harvesting chlorophyll-a/b binding protein LhcII-3 = LHCBM2 (summary available)
         Chlorophyll a-b binding protein of LHCII = LHCBM1 (summary available)
         CP26 = LHCB5 (summary available)
         Chlorophyll a-b binding protein CP29 = LHCB4 (summary available)
         OEC complex = (PSBQ)(PSBP1)(PSBO) (summary available)
                 Oxygen-evolving enhancer protein 3 = PSBQ (summary available)
                 Oxygen-evolving enhancer protein 2 of photosystem II = PSBP1 (summary available)
                 Oxygen-evolving enhancer protein 1 = PSBO (summary available)
         PS II core dimer = ([(PsbK)(PsbC)([PsbT][PsbR][PsbM][PsbL][PsbJ][PsbH][(PsbB)([PsbA][(PsbE)(PsbD)])])][PSBW][PsbZ][PSBO])2 (extended summary available)
                 PS II core monomer (complete) = ([PsbK][PsbC][(PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])])])(PSBW)(PsbZ)(PSBO)
                         PS II core monomer = (PsbK)(PsbC)([PsbT][PsbR][PsbM][PsbL][PsbJ][PsbH][(PsbB)([PsbA][(PsbE)(PsbD)])]) (summary available)
                                 Photosystem II reaction center protein K = PsbK (summary available)
                                 CP43 = PsbC (summary available)
                                 CP43-less PS II core monomer = (PsbT)(PsbR)(PsbM)(PsbL)(PsbJ)(PsbH)([PsbB][(PsbA)([PsbE][PsbD])]) (summary available)
                                         Photosystem II reaction center protein T = PsbT (summary available)
                                         10 kDa photosystem II polypeptide = PsbR (summary available)
                                         Photosystem II reaction center protein M = PsbM
                                         Photosystem II reaction center protein L = PsbL
                                         Photosystem II reaction center protein J = PsbJ (summary available)
                                         Photosystem II reaction center protein H = PsbH
                                         PS II RC - CP47 complex = (PsbB)([PsbA][(PsbE)(PsbD)]) (summary available)
                                                 Photosystem II CP47 chlorophyll apoprotein = PsbB (summary available)
                                                 PS II RC = (PsbA)([PsbE][PsbD])
                                                         Photosystem Q(B) protein = PsbA (summary available)
                                                         cytb559-D2 complex = (PsbE)(PsbD) (summary available)
                                                                 cytochrome b-559 α-subunit = PsbE (summary available)
                                                                 Photosystem II D2 protein = PsbD (summary available)
                         Photosystem II reaction center W protein = PSBW
                         Photosystem II reaction center protein Z = PsbZ (summary available)
                         Oxygen-evolving enhancer protein 1 = PSBO (summary available)

Summary:
The process of photosynthesis which is central to the presence of life on earth involves the interplay of several reactions. Chloroplasts are the pivotal organelles in this process and they are highly compartmentalized for this function which is mediated by three light dependet complexes. Photosystem I (PSI), photosystem II (PSII), and the cytochrome b6f complex are the three light-dependent complexes, located in the thylakoid membranes, a detailed network of flattened vesicles [Uniacke07].

Photosystem II is a water oxidizing multisubunit protein complex [Barber02]. A cluster of four manganese atoms bound to the lumenal surface of the complex catalyzes the water oxidizing reaction which is powered by solar energy. The Mn cluster is sequentially oxidized by P680 and a chlorophyll a molecule that acts as the primary electron donor, with pheophytin acting as the primary electron acceptor [Allakhverdiev11]. All these redox active components of PSII are bound to the two structurally related proteins known as D1 and D2. Associated with the D1/D2 heterodimer are two chlorophyll proteins CP43 and CP47 and at least three extrinsic proteins that protect and optimize the activity of the Mn cluster [Buchel99]. These proteins, cytochrome b559 and some other subunits make up the core complex [Hankamer01].

Citations: [Jun09, Hankamer01a]

Locations: chloroplast thylakoid membrane, chloroplast

GO Terms:

Cellular Component: GO:0009507 - chloroplast
GO:0042651 - thylakoid membrane

Credits:
Created 12-Jan-2010 by May P , MPI-MP
Revised 17-May-2011 by Weerasinghe D


Enzymatic reaction of: photosystem II

EC Number: 1.10.3.9

4 hν + 2 a plastoquinone + 2 H2O <=> 2 a plastoquinol + oxygen

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is physiologically favored in the direction shown.

In Pathways: photosynthesis light reactions , hydrogen production VIII


References

Allakhverdiev11: Allakhverdiev SI, Tsuchiya T, Watabe K, Kojima A, Los DA, Tomo T, Klimov VV, Mimuro M (2011). "Redox potentials of primary electron acceptor quinone molecule (QA)- and conserved energetics of photosystem II in cyanobacteria with chlorophyll a and chlorophyll d." Proc Natl Acad Sci U S A 108(19);8054-8. PMID: 21521792

Barber02: Barber J (2002). "Photosystem II: a multisubunit membrane protein that oxidises water." Curr Opin Struct Biol 12(4);523-30. PMID: 12163077

Buchel99: Buchel C, Barber J, Ananyev G, Eshaghi S, Watt R, Dismukes C (1999). "Photoassembly of the manganese cluster and oxygen evolution from monomeric and dimeric CP47 reaction center photosystem II complexes." Proc Natl Acad Sci U S A 96(25);14288-93. PMID: 10588698

Dey97: Dey, PM, Harborne, JB "Plant Biochemistry." Academic Press 1997.

Hankamer01: Hankamer B, Morris E, Nield J, Gerle C, Barber J (2001). "Three-dimensional structure of the photosystem II core dimer of higher plants determined by electron microscopy." J Struct Biol 135(3);262-9. PMID: 11722166

Hankamer01a: Hankamer B, Morris E, Nield J, Carne A, Barber J (2001). "Subunit positioning and transmembrane helix organisation in the core dimer of photosystem II." FEBS Lett 504(3);142-51. PMID: 11532446

Higgs98a: Higgs DC, Kuras R, Kindle KL, Wollman FA, Stern DB (1998). "Inversions in the Chlamydomonas chloroplast genome suppress a petD 5' untranslated region deletion by creating functional chimeric mRNAs." Plant J 14(6);663-71. PMID: 9681031

Jun09: Jun Minagawa (2009). "Light-Harvesting Proteins." Chapter 14. In: The Chlamydomonas Sourcebook. Second Edition.

Loll05: Loll B, Kern J, Saenger W, Zouni A, Biesiadka J (2005). "Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II." Nature 438(7070);1040-4. PMID: 16355230

Maul02: Maul JE, Lilly JW, Cui L, dePamphilis CW, Miller W, Harris EH, Stern DB (2002). "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats." Plant Cell 14(11);2659-79. PMID: 12417694

Nield00: Nield J, Kruse O, Ruprecht J, da Fonseca P, Buchel C, Barber J (2000). "Three-dimensional structure of Chlamydomonas reinhardtii and Synechococcus elongatus photosystem II complexes allows for comparison of their oxygen-evolving complex organization." J Biol Chem 275(36);27940-6. PMID: 10807922

Nixon94: Nixon PJ, Diner BA (1994). "Analysis of water-oxidation mutants constructed in the cyanobacterium Synechocystis sp. PCC 6803." Biochem Soc Trans 22(2);338-43. PMID: 7958321

Rokka05: Rokka A, Suorsa M, Saleem A, Battchikova N, Aro EM (2005). "Synthesis and assembly of thylakoid protein complexes: multiple assembly steps of photosystem II." Biochem J 388(Pt 1);159-68. PMID: 15638811

Sugimoto03: Sugimoto I, Takahashi Y (2003). "Evidence that the PsbK polypeptide is associated with the photosystem II core antenna complex CP43." J Biol Chem 278(45);45004-10. PMID: 12939265

Teramoto01: Teramoto H, Ono T, Minagawa J (2001). "Identification of Lhcb gene family encoding the light-harvesting chlorophyll-a/b proteins of photosystem II in Chlamydomonas reinhardtii." Plant Cell Physiol 42(8);849-56. PMID: 11522911

Uniacke07: Uniacke J, Zerges W (2007). "Photosystem II assembly and repair are differentially localized in Chlamydomonas." Plant Cell 19(11);3640-54. PMID: 18055604


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, biocyc13.