Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Protein: citrate lyase, citryl-ACP lyase component

Gene: citE Accession Numbers: G6342 (MetaCyc), b0616, ECK0609

Synonyms: ybdW

Species: Escherichia coli K-12 substr. MG1655

Component of: citrate lyase, inactive

Subunit composition of citrate lyase, citryl-ACP lyase component = [CitE]6
         citrate lyase, citryl-ACP lyase β subunit = CitE

Alternative forms of citrate lyase, inactive: citrate lyase (extended summary available)

Locations: cytosol

Map Position: [648,805 <- 649,713]

Molecular Weight of Polypeptide: 33.11 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002122 , EchoBASE:EB3312 , EcoGene:EG13542 , EcoliWiki:b0616 , Mint:MINT-1283346 , ModBase:P0A9I1 , OU-Microarray:b0616 , PortEco:citE , PR:PRO_000022290 , Pride:P0A9I1 , Protein Model Portal:P0A9I1 , RefSeq:NP_415149 , RegulonDB:G6342 , SMR:P0A9I1 , String:511145.b0616 , UniProt:P0A9I1

Relationship Links: InterPro:IN-FAMILY:IPR005000 , InterPro:IN-FAMILY:IPR006475 , InterPro:IN-FAMILY:IPR011206 , InterPro:IN-FAMILY:IPR015813 , Pfam:IN-FAMILY:PF03328

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006084 - acetyl-CoA metabolic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0003824 - catalytic activity Inferred by computational analysis [GOA01]
GO:0008815 - citrate (pro-3S)-lyase activity Inferred by computational analysis [GOA01a]
GO:0008816 - citryl-CoA lyase activity Inferred by computational analysis [GOA01a, GOA01]
GO:0016829 - lyase activity Inferred by computational analysis [UniProtGOA11]
GO:0046872 - metal ion binding Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]
GO:0009346 - citrate lyase complex Inferred by computational analysis [GOA01]

MultiFun Terms: metabolism central intermediary metabolism unassigned reversible reactions

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Subunit of: citrate lyase, inactive

Synonyms: (HS)-citrate lyase, citrate lyase, thiol form, deacetyl-citrate lyase

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of citrate lyase, inactive = [(CitD)6][(CitF)6][(CitE)6]
         citrate lyase [acyl carrier protein] component = (CitD)6 (summary available)
                 citrate lyase, acyl carrier γ subunit = CitD
         citrate lyase, citrate-ACP transferase component = (CitF)6
                 citrate lyase, citrate-ACP transferase α subunit = CitF
         citrate lyase, citryl-ACP lyase component = (CitE)6
                 citrate lyase, citryl-ACP lyase β subunit = CitE

Reactions known to consume the compound:

citrate lyase activation :
[a holo citrate lyase acyl-carrier protein] + S-acetyl phosphopantetheine → an acetyl-[holo citrate lyase acyl-carrier protein] + 4'-phosphopantetheine
[a holo citrate lyase acyl-carrier protein] + acetate + ATP → an acetyl-[holo citrate lyase acyl-carrier protein] + AMP + diphosphate

Reactions known to produce the compound:

citrate lyase activation :
an acetyl-[holo citrate lyase acyl-carrier protein] + 6 H2O → [a holo citrate lyase acyl-carrier protein] + 6 acetate
2'-(5''-triphospho-α-D-ribosyl)-3'-dephospho-CoA + [an apo citrate-lyase acyl-carrier protein] → [a holo citrate lyase acyl-carrier protein] + diphosphate

Credits:
Imported from EcoCyc 16-Sep-2014 by Paley S , SRI International


Sequence Features

Feature Class Location Citations Comment
Amino-Acid-Sites-That-Bind 76
[UniProt10a]
UniProt: Substrate; Non-Experimental Qualifier: by similarity;
Metal-Binding-Site 139
[UniProt10a]
UniProt: Magnesium; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 150 -> 156
[Ingmer96, UniProt10]
Alternate sequence: SLTLPRL; UniProt: (in Ref. 5; AAC28948);
Metal-Binding-Site 166
[UniProt10a]
UniProt: Magnesium; Non-Experimental Qualifier: by similarity;

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene right-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Ingmer96: Ingmer H., Cohen S.N. (1996). Data submission to EMBL/GenBank/DDBJ databases on 1996-01.

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, biocyc14.