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MetaCyc Polypeptide: 50S ribosomal subunit protein L14

Gene: rplN Accession Numbers: EG10875 (MetaCyc), b3310, ECK3297

Species: Escherichia coli K-12 substr. MG1655

Component of:
50S ribosomal subunit
ribosome

Summary:
The L14 protein is a component of the 50S subunit of the ribosome.

L14 crosslinks to 23S rRNA [Osswald90, Urlaub95]. In the cryo-EM reconstruction of the ribosome, L14 was modelled to be located at the 30S-50S subunit interface, at bridges B5 and B8 [Gao03]. In all-atom molecular dynamics simulations of the ribosome, a structural gate between helices 71 and 92 of 23S rRNA restricts tRNA motion and together with L14 acts as a steric filter for the cognate tRNA [Caulfield12].

L14 interacts directly with ribosomal silencing factor. Amino acid residues in L14 that are essential for this interaction have been identified by mutagenesis [Hauser12].

Based on amino-terminal sequencing of the purified protein, L14 may be identical to Rep helicase stimulatory protein (RHSP) [Smith89d, Yancey91]. RHSP forms aggregates on DNA and interacts with rRNA [Yancey91].

Retroregulation of L14 expression by mRNA processing and decay has been studied in E. coli B/r [Liang99].

Locations: cytosol, ribosome

Map Position: [3,447,778 <- 3,448,149]

Unification Links: ASAP:ABE-0010837, CGSC:252, DIP:DIP-35798N, EchoBASE:EB0868, EcoGene:EG10875, EcoliWiki:b3310, Mint:MINT-1304553, ModBase:P0ADY3, OU-Microarray:b3310, PortEco:rplN, PR:PRO_000023820, Pride:P0ADY3, Protein Model Portal:P0ADY3, RefSeq:NP_417769, RegulonDB:EG10875, SMR:P0ADY3, String:511145.b3310, Swiss-Model:P0ADY3, UniProt:P0ADY3

Relationship Links: InterPro:IN-FAMILY:IPR000218, InterPro:IN-FAMILY:IPR005745, InterPro:IN-FAMILY:IPR019972, InterPro:IN-FAMILY:IPR023571, Panther:IN-FAMILY:PTHR11761, PDB:Structure:1ML5, PDB:Structure:2J28, PDB:Structure:2RDO, PDB:Structure:3BBX, PDB:Structure:3IY9, PDB:Structure:3IZZ, PDB:Structure:3J5L, PDB:Structure:3J7Z, PDB:Structure:3J9Y, PDB:Structure:487D, PDB:Structure:4CSU, PDB:Structure:4U1U, PDB:Structure:4U1V, PDB:Structure:4U20, PDB:Structure:4U24, PDB:Structure:4U25, PDB:Structure:4U26, PDB:Structure:4U27, PDB:Structure:4UY8, PDB:Structure:4V6E, PDB:Structure:4V6K, PDB:Structure:4V6L, PDB:Structure:4V6M, PDB:Structure:4V6N, PDB:Structure:4V6O, PDB:Structure:4V6P, PDB:Structure:4V6Q, PDB:Structure:4V6R, PDB:Structure:4V6S, PDB:Structure:4V6T, PDB:Structure:4V6V, PDB:Structure:4V6Y, PDB:Structure:4V6Z, PDB:Structure:4V7V, PDB:Structure:4V9C, PDB:Structure:4V9D, PDB:Structure:4V9O, PDB:Structure:4V9P, PDB:Structure:4V47, PDB:Structure:4V48, PDB:Structure:4V4H, PDB:Structure:4V4Q, PDB:Structure:4V4V, PDB:Structure:4V4W, PDB:Structure:4V50, PDB:Structure:4V52, PDB:Structure:4V53, PDB:Structure:4V54, PDB:Structure:4V55, PDB:Structure:4V56, PDB:Structure:4V57, PDB:Structure:4V5B, PDB:Structure:4V5H, PDB:Structure:4V5Y, PDB:Structure:4V64, PDB:Structure:4V65, PDB:Structure:4V66, PDB:Structure:4V69, PDB:Structure:4V6C, PDB:Structure:4V6D, PDB:Structure:4V70, PDB:Structure:4V71, PDB:Structure:4V72, PDB:Structure:4V73, PDB:Structure:4V74, PDB:Structure:4V75, PDB:Structure:4V76, PDB:Structure:4V77, PDB:Structure:4V78, PDB:Structure:4V79, PDB:Structure:4V7A, PDB:Structure:4V7B, PDB:Structure:4V7C, PDB:Structure:4V7D, PDB:Structure:4V7I, PDB:Structure:4V7S, PDB:Structure:4V7T, PDB:Structure:4V7U, PDB:Structure:4V85, PDB:Structure:4V89, PDB:Structure:4WF1, PDB:Structure:4WWW, PDB:Structure:4YBB, PDB:Structure:5AFI, PDB:Structure:5AKA, Pfam:IN-FAMILY:PF00238, Prosite:IN-FAMILY:PS00049

Gene-Reaction Schematic

Gene-Reaction Schematic


GO Terms:
Biological Process:
Inferred by computational analysisGO:0006412 - translation [GOA06, GOA01a]
Molecular Function:
Inferred from experimentGO:0005515 - protein binding [Hauser12]
Inferred from experimentGO:0070180 - large ribosomal subunit rRNA binding [Urlaub95]
Inferred by computational analysisGO:0003723 - RNA binding [UniProtGOA11a]
Inferred by computational analysisGO:0003735 - structural constituent of ribosome [Gaudet10, GOA01a]
Inferred by computational analysisGO:0019843 - rRNA binding [UniProtGOA11a, GOA06]
Cellular Component:
Inferred from experimentInferred by computational analysisGO:0005829 - cytosol [DiazMejia09, Ishihama08]
Inferred from experimentGO:0022625 - cytosolic large ribosomal subunit [Hindennach71a, Kaltschmidt70]
Inferred by computational analysisGO:0005840 - ribosome [UniProtGOA11a, GOA01a]
Inferred by computational analysisGO:0015934 - large ribosomal subunit [GOA01a]
Inferred by computational analysisGO:0030529 - intracellular ribonucleoprotein complex [UniProtGOA11a]

MultiFun Terms: cell structureribosomes
information transferprotein relatedribosomal proteins
information transferprotein relatedtranslation

Credits:
Last-Curated 05-Apr-2006 by Keseler I, SRI International


Subunit of: 50S ribosomal subunit

Synonyms: ribosome, large subunit

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of 50S ribosomal subunit = [RplA][RplB][RplC][RplD][RplE][RplF][(RplJ)([RplL]2)2][RplI][RplJ][RplK][RplM][RplN][RplO][RplP][RplQ][RplR][RplS][RplT][RplU][RplV][RplW][RplX][RplY][RpmA][RpmB][RpmC][RpmD][RpmE][RpmF][RpmG][RpmH][RpmI][RpmJ][5S ribosomal RNA (rrfA)][23S ribosomal RNA (rrlA)]
         50S ribosomal subunit protein L1 = RplA (extended summary available)
         50S ribosomal subunit protein L2 = RplB (summary available)
         50S ribosomal subunit protein L3 = RplC (summary available)
         50S ribosomal subunit protein L4 = RplD (extended summary available)
         50S ribosomal subunit protein L5 = RplE (extended summary available)
         50S ribosomal subunit protein L6 = RplF (summary available)
         50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                         50S ribosomal subunit protein L12 = RplL
         50S ribosomal subunit protein L9 = RplI (extended summary available)
         50S ribosomal subunit protein L10 = RplJ (extended summary available)
         50S ribosomal subunit protein L11 = RplK (extended summary available)
         50S ribosomal subunit protein L13 = RplM (extended summary available)
         50S ribosomal subunit protein L14 = RplN (extended summary available)
         50S ribosomal subunit protein L15 = RplO (summary available)
         50S ribosomal subunit protein L16 = RplP (extended summary available)
         50S ribosomal subunit protein L17 = RplQ (summary available)
         50S ribosomal subunit protein L18 = RplR (extended summary available)
         50S ribosomal subunit protein L19 = RplS (extended summary available)
         50S ribosomal subunit protein L20 = RplT (extended summary available)
         50S ribosomal subunit protein L21 = RplU (summary available)
         50S ribosomal subunit protein L22 = RplV (extended summary available)
         50S ribosomal subunit protein L23 = RplW (extended summary available)
         50S ribosomal subunit protein L24 = RplX (summary available)
         50S ribosomal subunit protein L25 = RplY (extended summary available)
         50S ribosomal subunit protein L27 = RpmA (extended summary available)
         50S ribosomal subunit protein L28 = RpmB (summary available)
         50S ribosomal subunit protein L29 = RpmC (summary available)
         50S ribosomal subunit protein L30 = RpmD (summary available)
         50S ribosomal subunit protein L31 = RpmE (summary available)
         50S ribosomal subunit protein L32 = RpmF (summary available)
         50S ribosomal subunit protein L33 = RpmG (extended summary available)
         50S ribosomal subunit protein L34 = RpmH (summary available)
         50S ribosomal subunit protein L35 = RpmI (summary available)
         50S ribosomal subunit protein L36 = RpmJ (extended summary available)

Component of: ribosome

Enzymatic reactions activated by 50S ribosomal subunit, sorted by the type of activation, are:

Activator (Mechanism unknown) of: GTPase [Jain09, Shields09]

Credits:
Created 02-Jun-2006 by Keseler I, SRI International


Subunit of: ribosome

Species: Escherichia coli K-12 substr. MG1655

Subunit composition of ribosome = [(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))][(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))]
         30S ribosomal subunit = (RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)(16S ribosomal RNA (rrsA))
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplJ)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)(5S ribosomal RNA (rrfA))(23S ribosomal RNA (rrlA))
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (extended summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (extended summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (extended summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (extended summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (extended summary available)

Enzymatic reactions activated by ribosome, sorted by the type of activation, are:

Activator (Mechanism unknown) of: ATPase [Becker12], GTPase [Shields09], GTPase, ribosome-stimulated [De15]

Credits:
Created 15-Jun-2006 by Keseler I, SRI International


Sequence Features

Feature Class Location Citations Comment
Sequence-Conflict 63
Inferred by curator[Morinaga78, UniProt15]
UniProt: (in Ref. 1; AA sequence).
Sequence-Conflict 84
Inferred by curator[Morinaga78, UniProt15]
UniProt: (in Ref. 1; AA sequence).
Sequence-Conflict 90 -> 91
Inferred by curator[Morinaga78, UniProt15]
UniProt: (in Ref. 1; AA sequence).
Sequence-Conflict 97
Inferred by curator[Morinaga78, UniProt15]
UniProt: (in Ref. 1; AA sequence).
Mutagenesis-Variant 97
Inferred from experiment[Hauser12]
UniProt: Reduced RsfS binding.
Mutagenesis-Variant 98
Inferred from experiment[Hauser12]
UniProt: Reduced RsfS binding.
Mutagenesis-Variant 114
Inferred from experiment[Hauser12]
UniProt: Reduced RsfS binding.
Sequence-Conflict 115
Inferred by curator[Morinaga78, UniProt15]
UniProt: (in Ref. 1; AA sequence).
Mutagenesis-Variant 117
Inferred from experiment[Hauser12]
UniProt: No change in RsfS binding.
Sequence-Conflict 123
Inferred by curator[Morinaga78, UniProt15]
UniProt: (in Ref. 1; AA sequence).


Sequence Pfam Features

Feature Class Location Citations Comment
Pfam PF00238 1 -> 123
Inferred by computational analysis[Finn14]
Ribosomal_L14 : Ribosomal protein L14p/L23e

History:
10/20/97 Gene b3310 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10875; confirmed by SwissProt match.


References

Becker12: Becker M, Gzyl KE, Altamirano AM, Vuong A, Urban K, Wieden HJ (2012). "The 70S ribosome modulates the ATPase activity of Escherichia coli YchF." RNA Biol 9(10);1288-301. PMID: 22995830

Caulfield12: Caulfield T, Devkota B (2012). "Motion of transfer RNA from the A/T state into the A-site using docking and simulations." Proteins 80(11);2489-500. PMID: 22730134

De15: De Laurentiis EI, Wieden HJ (2015). "Identification of two structural elements important for ribosome-dependent GTPase activity of elongation factor 4 (EF4/LepA)." Sci Rep 5;8573. PMID: 25712150

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Finn14: Finn RD, Bateman A, Clements J, Coggill P, Eberhardt RY, Eddy SR, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer EL, Tate J, Punta M (2014). "Pfam: the protein families database." Nucleic Acids Res 42(Database issue);D222-30. PMID: 24288371

Gao03: Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg SM, Van Roey P, Agrawal RK, Harvey SC, Sali A, Chapman MS, Frank J (2003). "Study of the structural dynamics of the E coli 70S ribosome using real-space refinement." Cell 113(6);789-801. PMID: 12809609

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Hauser12: Hauser R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, Tovchigrechko A, Yamamoto K, Szaflarski W, Takeuchi N, Stellberger T, Diefenbacher ME, Nierhaus KH, Uetz P (2012). "RsfA (YbeB) proteins are conserved ribosomal silencing factors." PLoS Genet 8(7);e1002815. PMID: 22829778

Hindennach71a: Hindennach I, Kaltschmidt E, Wittmann HG (1971). "Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);12-6. PMID: 4942547

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Jain09: Jain N, Dhimole N, Khan AR, De D, Tomar SK, Sajish M, Dutta D, Parrack P, Prakash B (2009). "E. coli HflX interacts with 50S ribosomal subunits in presence of nucleotides." Biochem Biophys Res Commun 379(2);201-5. PMID: 19109926

Kaltschmidt70: Kaltschmidt E, Wittmann HG (1970). "Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis." Proc Natl Acad Sci U S A 67(3);1276-82. PMID: 4922286

Liang99: Liang ST, Ehrenberg M, Dennis P, Bremer H (1999). "Decay of rplN and lacZ mRNA in Escherichia coli." J Mol Biol 288(4);521-38. PMID: 10329160

Morinaga78: Morinaga T, Funatsu G, Funatsu M (1978). "Primary structure of protein L14 isolated from Escherichia coli ribosomes." FEBS Lett 91(1);74-7. PMID: 352727

Osswald90: Osswald M, Greuer B, Brimacombe R (1990). "Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents." Nucleic Acids Res 18(23);6755-60. PMID: 1702198

Shields09: Shields MJ, Fischer JJ, Wieden HJ (2009). "Toward understanding the function of the universally conserved GTPase HflX from Escherichia coli: a kinetic approach." Biochemistry 48(45);10793-802. PMID: 19824612

Smith89d: Smith KR, Yancey JE, Matson SW (1989). "Identification and purification of a protein that stimulates the helicase activity of the Escherichia coli Rep protein." J Biol Chem 264(11);6119-26. PMID: 2522929

UniProt15: UniProt Consortium (2015). "UniProt version 2015-08 released on 2015-07-22." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Urlaub95: Urlaub H, Kruft V, Bischof O, Muller EC, Wittmann-Liebold B (1995). "Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." EMBO J 14(18);4578-88. PMID: 7556101

Yancey91: Yancey JE, Matson SW (1991). "The DNA unwinding reaction catalyzed by Rep protein is facilitated by an RHSP-DNA interaction." Nucleic Acids Res 19(14);3943-51. PMID: 1650456


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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