MetaCyc Enzyme: adenylylsulfate:phosphate adenylyltransferase

Gene: apt Accession Number: G-9571 (MetaCyc)

Species: Thiobacillus denitrificans

Subunit composition of adenylylsulfate:phosphate adenylyltransferase = [Apt]2
         adenylylsulfate:phosphate adenylyltransferase subunit = Apt

adenylylsulfate:phosphate adenylyltransferase (APAT) was isolated from the chemolithoautotroph Thiobacillus denitrificans, and the apt gene encoding it was cloned [Bruser00].

The enzyme is homodimeric and exhibits a specific activity of 2100 μmol/min/mg. Catalysis occurs by a ping-pong mechanism with a covalently bound AMP as reaction intermediate. adenylylsulfate:phosphate adenylyltransferase is highly similar to galactose-1-phosphate uridylyltransferase and is also related to ATP adenylyltransferase. The active site residues of galactose-1-phosphate uridylyltransferase are conserved in all three enzymes, suggesting a histidine as the nucleotide-binding residue in all of them [Bruser00].

Molecular Weight of Polypeptide: 41.518 kD (from nucleotide sequence), 41.387 kD (experimental) [Bruser00]

Unification Links: Protein Model Portal:Q9LA72, UniProt:Q9LA72

Relationship Links: Entrez-Nucleotide:PART-OF:AF148553, InterPro:IN-FAMILY:IPR001937, InterPro:IN-FAMILY:IPR005849, InterPro:IN-FAMILY:IPR011146, Panther:IN-FAMILY:PTHR11943, Pfam:IN-FAMILY:PF01087

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 11-Sep-2006 by Caspi R, SRI International

Enzymatic reaction of: adenylylsulfate:phosphate adenylyltransferase

Inferred from experiment

EC Number:

sulfate + ADP + H+ ← adenosine 5'-phosphosulfate + phosphate

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: sulfite oxidation II

Kinetic Parameters:
Substrate Km (μM) Citations
phosphate 12000.0 [Bruser00]
adenosine 5'-phosphosulfate 300.0 [Bruser00]


Bruser00: Bruser T, Selmer T, Dahl C (2000). ""ADP sulfurylase" from Thiobacillus denitrificans is an adenylylsulfate:phosphate adenylyltransferase and belongs to a new family of nucleotidyltransferases." J Biol Chem 275(3);1691-8. PMID: 10636864

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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