|Gene:||apt||Accession Number: G-9571 (MetaCyc)|
Species: Thiobacillus denitrificans
Subunit composition of
adenylylsulfate:phosphate adenylyltransferase = [Apt]2
adenylylsulfate:phosphate adenylyltransferase subunit = Apt
The enzyme is homodimeric and exhibits a specific activity of 2100 μmol/min/mg. Catalysis occurs by a ping-pong mechanism with a covalently bound AMP as reaction intermediate. adenylylsulfate:phosphate adenylyltransferase is highly similar to galactose-1-phosphate uridylyltransferase and is also related to ATP adenylyltransferase. The active site residues of galactose-1-phosphate uridylyltransferase are conserved in all three enzymes, suggesting a histidine as the nucleotide-binding residue in all of them [Bruser00].
Molecular Weight of Polypeptide: 41.518 kD (from nucleotide sequence), 41.387 kD (experimental) [Bruser00]
Enzymatic reaction of: adenylylsulfate:phosphate adenylyltransferase
EC Number: 18.104.22.168sulfate + ADP + H+ ← adenosine 5'-phosphosulfate + phosphate
The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.
The reaction is favored in the opposite direction.
In Pathways: sulfite oxidation IIKinetic Parameters:
Bruser00: Bruser T, Selmer T, Dahl C (2000). ""ADP sulfurylase" from Thiobacillus denitrificans is an adenylylsulfate:phosphate adenylyltransferase and belongs to a new family of nucleotidyltransferases." J Biol Chem 275(3);1691-8. PMID: 10636864
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