|Gene:||apt||Accession Number: G-9571 (MetaCyc)|
Species: Thiobacillus denitrificans
Subunit composition of
adenylylsulfate:phosphate adenylyltransferase = [Apt]2
adenylylsulfate:phosphate adenylyltransferase subunit = Apt
The enzyme is homodimeric and exhibits a specific activity of 2100 μmol/min/mg. Catalysis occurs by a ping-pong mechanism with a covalently bound AMP as reaction intermediate. adenylylsulfate:phosphate adenylyltransferase is highly similar to galactose-1-phosphate uridylyltransferase and is also related to ATP adenylyltransferase. The active site residues of galactose-1-phosphate uridylyltransferase are conserved in all three enzymes, suggesting a histidine as the nucleotide-binding residue in all of them [Bruser00].
Molecular Weight of Polypeptide: 41.518 kD (from nucleotide sequence), 41.387 kD (experimental) [Bruser00 ]
Relationship Links: Entrez-Nucleotide:PART-OF:AF148553 , InterPro:IN-FAMILY:IPR001937 , InterPro:IN-FAMILY:IPR005849 , InterPro:IN-FAMILY:IPR011146 , Panther:IN-FAMILY:PTHR11943 , Pfam:IN-FAMILY:PF01087
Enzymatic reaction of: adenylylsulfate:phosphate adenylyltransferase
EC Number: 188.8.131.52
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.
The reaction is favored in the opposite direction.
In Pathways: sulfite oxidation II
Bruser00: Bruser T, Selmer T, Dahl C (2000). ""ADP sulfurylase" from Thiobacillus denitrificans is an adenylylsulfate:phosphate adenylyltransferase and belongs to a new family of nucleotidyltransferases." J Biol Chem 275(3);1691-8. PMID: 10636864
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