MetaCyc Enzyme: β-ketoadipyl CoA thiolase

Gene: pcaF Accession Number: G-9823 (MetaCyc)

Species: Pseudomonas knackmussii

Subunit composition of β-ketoadipyl CoA thiolase = [PcaF]4
         β-ketoadipyl CoA thiolase subunit = PcaF

β-ketoadipyl CoA thiolase has been purified from Pseudomonas knackmussii and found to be a homotetramer [Kaschabek02]. Sequence analysis of the thiolase terminus revealed high percentages of identity (70 to 85%) with thiolases of different functions.

The thiolase was able to participate in the degradation of 3-oxoadipate, but not chlorosubstituted compounds, which are also known to be degraded by this organism [Kaschabek02].

Molecular Weight of Polypeptide: 42 kD (experimental) [Kaschabek02]

Molecular Weight of Multimer: 162 kD (experimental) [Kaschabek02]

Gene-Reaction Schematic

Gene-Reaction Schematic

Created 15-Dec-2006 by Caspi R, SRI International

Enzymatic reaction of: β-ketoadipyl CoA thiolase

Inferred from experiment

EC Number:

succinyl-CoA + acetyl-CoA ⇄ 3-oxoadipyl-CoA + coenzyme A

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: catechol degradation III (ortho-cleavage pathway)

Inhibitors (Uncompetitive): 2-chloro-3-oxoadipate [Kaschabek02], 2-hydroxy-3-oxoadipate [Kaschabek02], 5-chloro-4-oxopentanoate [Kaschabek02] Inhibitors (Other): Cu2+ [Kaschabek02], hydrogen cyanide [Kaschabek02], Zn2+ [Kaschabek02]Kinetic Parameters:
Substrate Km (μM) Citations
3-oxoadipyl-CoA 150.0 [Kaschabek02]
coenzyme A 10.0 [Kaschabek02]

pH(opt): 7.8 [Kaschabek02]


Kaschabek02: Kaschabek SR, Kuhn B, Muller D, Schmidt E, Reineke W (2002). "Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase." J Bacteriol 184(1);207-15. PMID: 11741862

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Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 19.5 on Wed Nov 25, 2015, BIOCYC14A.