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MetaCyc Enzyme: β-ketoadipyl CoA thiolase

Gene: pcaF Accession Number: G-9823 (MetaCyc)

Species: Pseudomonas knackmussii

Subunit composition of β-ketoadipyl CoA thiolase = [PcaF]4
         β-ketoadipyl CoA thiolase subunit = PcaF

Summary:
β-ketoadipyl CoA thiolase has been purified from Pseudomonas knackmussii and found to be a homotetramer [Kaschabek02]. Sequence analysis of the thiolase terminus revealed high percentages of identity (70 to 85%) with thiolases of different functions.

The thiolase was able to participate in the degradation of 3-oxoadipate, but not chlorosubstituted compounds, which are also known to be degraded by this organism [Kaschabek02].

Molecular Weight of Polypeptide: 42 kD (experimental) [Kaschabek02 ]

Molecular Weight of Multimer: 162 kD (experimental) [Kaschabek02]

Gene-Reaction Schematic: ?

Credits:
Created 15-Dec-2006 by Caspi R , SRI International


Enzymatic reaction of: β-ketoadipyl CoA thiolase

EC Number: 2.3.1.174

succinyl-CoA + acetyl-CoA <=> 3-oxoadipyl-CoA + coenzyme A

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the opposite direction.

In Pathways: catechol degradation III (ortho-cleavage pathway)

Inhibitors (Uncompetitive): 2-chloro-3-oxoadipate [Kaschabek02] , 2-hydroxy-3-oxoadipate [Kaschabek02] , 5-chloro-4-oxopentanoate [Kaschabek02]

Inhibitors (Other): Cu2+ [Kaschabek02] , hydrogen cyanide [Kaschabek02] , Zn2+ [Kaschabek02]

Kinetic Parameters:

Substrate
Km (μM)
Citations
3-oxoadipyl-CoA
150.0
[Kaschabek02]
coenzyme A
10.0
[Kaschabek02]

pH(opt): 7.8 [Kaschabek02]


References

Kaschabek02: Kaschabek SR, Kuhn B, Muller D, Schmidt E, Reineke W (2002). "Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase." J Bacteriol 184(1);207-15. PMID: 11741862


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.