MetaCyc Polypeptide: dimethylmaleate hydratase subunit A

Gene: dmdA Accession Number: G-10436 (MetaCyc)

Species: Eubacterium barkeri

Component of: dimethylmaleate hydratase (summary available)

Gene Citations: [Alhapel06]

Molecular Weight of Polypeptide: 44.821 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q0QLE2 , UniProt:Q0QLE2

Relationship Links: Entrez-Nucleotide:RELATED-TO:DQ310789 , InterPro:IN-FAMILY:IPR001030 , InterPro:IN-FAMILY:IPR006251 , InterPro:IN-FAMILY:IPR011823 , InterPro:IN-FAMILY:IPR011826 , InterPro:IN-FAMILY:IPR015931 , InterPro:IN-FAMILY:IPR015932 , InterPro:IN-FAMILY:IPR015937 , InterPro:IN-FAMILY:IPR018136 , Panther:IN-FAMILY:PTHR11670 , Pfam:IN-FAMILY:PF00330 , Prints:IN-FAMILY:PR00415 , Prosite:IN-FAMILY:PS00450 , Prosite:IN-FAMILY:PS01244

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Subunit of: dimethylmaleate hydratase

Species: Eubacterium barkeri

Subunit composition of dimethylmaleate hydratase = [DmdA][DmdB]
         dimethylmaleate hydratase subunit A = DmdA
         dimethylmaleate hydratase subunit B = DmdB

The native molecular weight of the partially purified enzyme was estimated by gel filtration chromatography. However, the subunit composition was not determined [KollmannKoch84].

Enzymatic reaction of: dimethylmaleate hydratase

Synonyms: (2R,3S)-dimethylmalate dehydratase, (2R,3S)-2,3-dimethylmalate hydro-lyase

EC Number:

(2R,3S)-2,3-dimethylmalate <=> dimethylmaleate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for dimethylmaleate: citraconate [KollmannKoch84 ]

In Pathways: superpathway of nicotinate degradation , nicotinate degradation III

The enzyme is induced by growth of the organism on nicotinate. It is oxygen-sensitive and is dependent upon ferrous ion. The reaction was reversible, but the equilibrium was 70% in favor of dimethylmalate. This product was determined to have a (2R,3S) configuration [KollmannKoch84].

Cofactors or Prosthetic Groups: Fe2+ [KollmannKoch84]

Inhibitors (Unknown Mechanism): 5,5'-dithio-bis-2-nitrobenzoate [KollmannKoch84] , N-ethylmaleimide [KollmannKoch84]

Kinetic Parameters:

Km (μM)


Alhapel06: Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ (2006). "Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri." Proc Natl Acad Sci U S A 103(33);12341-6. PMID: 16894175

KollmannKoch84: Kollmann-Koch A, Eggerer H (1984). "Nicotinic acid metabolism. Dimethylmaleate hydratase." Hoppe Seylers Z Physiol Chem 365(8);847-57. PMID: 6489933

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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