MetaCyc Polypeptide: dimethylmaleate hydratase subunit B

Gene: dmdB Accession Number: G-10437 (MetaCyc)

Species: Eubacterium barkeri

Component of: dimethylmaleate hydratase (summary available)

Gene Citations: [Alhapel06]

Molecular Weight of Polypeptide: 17.369 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q0QLE1, UniProt:Q0QLE1

Relationship Links: Entrez-Nucleotide:RELATED-TO:DQ310789, InterPro:IN-FAMILY:IPR000573, InterPro:IN-FAMILY:IPR011824, InterPro:IN-FAMILY:IPR011827, InterPro:IN-FAMILY:IPR015928, InterPro:IN-FAMILY:IPR015937, Panther:IN-FAMILY:PTHR11670, Pfam:IN-FAMILY:PF00694

Gene-Reaction Schematic

Gene-Reaction Schematic

Subunit of: dimethylmaleate hydratase

Species: Eubacterium barkeri

Subunit composition of dimethylmaleate hydratase = [DmdA][DmdB]
         dimethylmaleate hydratase subunit A = DmdA
         dimethylmaleate hydratase subunit B = DmdB

The native molecular weight of the partially purified enzyme was estimated by gel filtration chromatography. However, the subunit composition was not determined [KollmannKoch84].

Enzymatic reaction of: dimethylmaleate hydratase

Inferred from experiment

Synonyms: (2R,3S)-dimethylmalate dehydratase, (2R,3S)-2,3-dimethylmalate hydro-lyase

EC Number:

(2R,3S)-2,3-dimethylmalate ⇄ dimethylmaleate + H2O

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for dimethylmaleate: citraconate [KollmannKoch84]

In Pathways: superpathway of nicotinate degradation, nicotinate degradation III

The enzyme is induced by growth of the organism on nicotinate. It is oxygen-sensitive and is dependent upon ferrous ion. The reaction was reversible, but the equilibrium was 70% in favor of dimethylmalate. This product was determined to have a (2R,3S) configuration [KollmannKoch84].

Cofactors or Prosthetic Groups: Fe2+ [KollmannKoch84]

Inhibitors (Unknown Mechanism): 5,5'-dithio-bis-2-nitrobenzoate [KollmannKoch84], N-ethylmaleimide [KollmannKoch84]Kinetic Parameters:
Substrate Km (μM) Citations
dimethylmaleate 3100.0 [KollmannKoch84]


Alhapel06: Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ (2006). "Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri." Proc Natl Acad Sci U S A 103(33);12341-6. PMID: 16894175

KollmannKoch84: Kollmann-Koch A, Eggerer H (1984). "Nicotinic acid metabolism. Dimethylmaleate hydratase." Hoppe Seylers Z Physiol Chem 365(8);847-57. PMID: 6489933

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
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