Metabolic Modeling Tutorial
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Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
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MetaCyc Polypeptide: dimethylmaleate hydratase subunit B

Gene: dmdB Accession Number: G-10437 (MetaCyc)

Species: Eubacterium barkeri

Component of: dimethylmaleate hydratase (summary available)

Gene Citations: [Alhapel06]

Molecular Weight of Polypeptide: 17.369 kD (from nucleotide sequence)

Unification Links: Protein Model Portal:Q0QLE1 , UniProt:Q0QLE1

Relationship Links: Entrez-Nucleotide:RELATED-TO:DQ310789 , InterPro:IN-FAMILY:IPR000573 , InterPro:IN-FAMILY:IPR011824 , InterPro:IN-FAMILY:IPR011827 , InterPro:IN-FAMILY:IPR015928 , InterPro:IN-FAMILY:IPR015937 , Panther:IN-FAMILY:PTHR11670 , Pfam:IN-FAMILY:PF00694

Gene-Reaction Schematic: ?


Subunit of: dimethylmaleate hydratase

Species: Eubacterium barkeri

Subunit composition of dimethylmaleate hydratase = [DmdA][DmdB]
         dimethylmaleate hydratase subunit A = DmdA
         dimethylmaleate hydratase subunit B = DmdB

Summary:
The native molecular weight of the partially purified enzyme was estimated by gel filtration chromatography. However, the subunit composition was not determined [KollmannKoch84].


Enzymatic reaction of: dimethylmaleate hydratase

Synonyms: (2R,3S)-dimethylmalate dehydratase, (2R,3S)-2,3-dimethylmalate hydro-lyase

EC Number: 4.2.1.85

(2R,3S)-2,3-dimethylmalate <=> dimethylmaleate + H2O

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction of enzyme catalysis.

This reaction is reversible.

Alternative Substrates for dimethylmaleate: citraconate [KollmannKoch84 ]

In Pathways: superpathway of nicotinate degradation , nicotinate degradation III

Summary:
The enzyme is induced by growth of the organism on nicotinate. It is oxygen-sensitive and is dependent upon ferrous ion. The reaction was reversible, but the equilibrium was 70% in favor of dimethylmalate. This product was determined to have a (2R,3S) configuration [KollmannKoch84].

Cofactors or Prosthetic Groups: Fe2+ [KollmannKoch84]

Inhibitors (Unknown Mechanism): 5,5'-dithio-bis-2-nitrobenzoate [KollmannKoch84] , N-ethylmaleimide [KollmannKoch84]

Kinetic Parameters:

Substrate
Km (μM)
Citations
dimethylmaleate
3100.0
[KollmannKoch84]


References

Alhapel06: Alhapel A, Darley DJ, Wagener N, Eckel E, Elsner N, Pierik AJ (2006). "Molecular and functional analysis of nicotinate catabolism in Eubacterium barkeri." Proc Natl Acad Sci U S A 103(33);12341-6. PMID: 16894175

KollmannKoch84: Kollmann-Koch A, Eggerer H (1984). "Nicotinic acid metabolism. Dimethylmaleate hydratase." Hoppe Seylers Z Physiol Chem 365(8);847-57. PMID: 6489933


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, BIOCYC14A.