MetaCyc Polypeptide: dipicolinate synthase β subunit

Gene: dpaB Accession Number: G-5067 (MetaCyc)

Species: Bacillus subtilis

Component of: dipicolinate synthase (summary available)

Molecular Weight of Polypeptide: 21.87 kD (from nucleotide sequence), 23 kD (experimental) [Daniel93 ]

Unification Links: ModBase:Q04810, Protein Model Portal:Q04810, SMR:Q04810, String:224308.Bsubs1_010100009221, Swiss-Model:Q04810, UniProt:Q04810

Relationship Links: Entrez-Nucleotide:RELATED-TO:Z22554, InterPro:IN-FAMILY:IPR003382, InterPro:IN-FAMILY:IPR014214, Pfam:IN-FAMILY:PF02441

Gene-Reaction Schematic

Gene-Reaction Schematic

MultiFun Terms: metabolismbiosynthesis of macromolecules (cellular constituents)

Subunit of: dipicolinate synthase

Species: Bacillus subtilis

Subunit composition of dipicolinate synthase = [DpaA][DpaB]
         dipicolinate synthase α subunit = DpaA
         dipicolinate synthase β subunit = DpaB

Dipicolinate is a small molecule that accumulates to high concentrations in bacterial endospores. It is synthesized in a single reaction by dipicolinate synthase from (S)-2,3-dihydrodipicolinate, a potential intermediate formed by 4-hydroxy-tetrahydrodipicolinate reductase, an enzyme that participates in the lysine and meso-DAP biosynthetic pathways.

In Bacillus subtilis the enzyme is composed of two subunits, encoded by the dpaA and dpaB genes. The enzyme has not been purified from B. subtilis, but the genes have been cloned and expressed in Escherichia coli, and an in vitro assay has confirmed that the gene products indeed catalyze dipicolinate synthase activity [Daniel93].

Enzymatic reaction of: dipicolinate synthase

Inferred from experiment

EC Number: 1.3.1.-

(S)-2,3-dihydrodipicolinate + NAD+ ⇄ dipicolinate + NADH + H+

The direction shown, i.e. which substrates are on the left and right sides, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.


Daniel93: Daniel RA, Errington J (1993). "Cloning, DNA sequence, functional analysis and transcriptional regulation of the genes encoding dipicolinic acid synthetase required for sporulation in Bacillus subtilis." J Mol Biol 232(2);468-83. PMID: 8345520

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of MetaCyc: Caspi et al, Nucleic Acids Research 42:D459-D471 2014
Page generated by Pathway Tools version 20.0 (software by SRI International) on Fri May 6, 2016, BIOCYC13B.